The EMBO Journal - Table of Contents alert Volume 31 Issue 6

TABLE OF CONTENTS 21 March 2012 | Volume 31, Issue 6 Have you seen? Focus Review Articles Corrigenda Also new AOP Sign up for e-alerts Recommend to your library Web feed Subscribe Advertisement In an iDEAL world, ChIP-Seq would work Diagenode now offers the powerful, complete iDEAL ChIP-Seq kit, the only kit on the market validated for GAIIx (Illumina®) and PGM™ (Ion Torrent™) sequencers. Our long history in developing optimal epigenetics tools and our expertise with ChIP-seq enables reproducible, efficient, and high quality results every time. Learn more about the iDEAL ChIP-Seq kit.   Have you seen? Top Ready, STAT, go: transcription factors on the move Ben-Yaakov and colleagues show a new pathway for neuronal survival by dynein-mediated nuclear transport of transcription factors translated in injured axons. Valentina Di Liberto and Valeria Cavalli The EMBO Journal (2012), 31, 1331 - 1333; 10.1038/emboj.2012.34 Abstract | Full text | PDF Published online: 14 February 2012 A GAIN in understanding autoproteolytic G protein-coupled receptors and polycystic kidney disease proteins Crystal structure work on a poorly defined class of adhesion GPCRs identifies a novel, evolutionary conserved GAIN domain that also features mutations in multiple human diseases. John J G Tesmer The EMBO Journal (2012), 31, 1334 - 1335; 10.1038/emboj.2012.51 Abstract | Full text | PDF Published online: 02 March 2012 Focus Review Top Mitochondrial quality control: a matter of life and death for neurons Mitochondrial integrity and functionality is monitored via multiple levels of cellular and organellar quality control that critically depend on mitochondrial proteases. Defects in these surveillance mechanisms cause neuronal loss in a number of prevalent neurodegenerative diseases. Elena I Rugarli and Thomas Langer The EMBO Journal (2012), 31, 1336 - 1349; 10.1038/emboj.2012.38 Abstract | Full text | PDF Published online: 21 February 2012 Subject Categories: Membranes and Transport | Neuroscience | Molecular Biology of Disease Articles Top Axonal transcription factors signal retrogradely in lesioned peripheral nerve The transcription factor STAT3 is locally translated in axons upon injury and is transported to the cell body by the importin-dynein complex to modulate survival of peripheral sensory neurons. Keren Ben-Yaakov, Mike Fainzilber and colleagues The EMBO Journal (2012), 31, 1350 - 1363; 10.1038/emboj.2011.494 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 January 2012 Subject Categories: Signal Transduction | Neuroscience A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis Crystallographic structures encompassing GPCR autoproteolytic sequences (GPS) delineate a novel conserved structural domain called GAIN, which is found in cell-adhesion GPCRs, polycystic kidney disease proteins conserved throughout evolution. Demet Araç, Axel T Brunger and colleagues The EMBO Journal (2012), 31, 1364 - 1378; 10.1038/emboj.2012.26 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 14 February 2012 Subject Categories: Signal Transduction | Structural Biology Hypoxia regulates glutamate receptor trafficking through an HIF-independent mechanism Hypoxia regulates the trafficking of the glutamate receptor GLR-1 in C. elegans neurons. This effect depends on the oxygen sensor Egl-9 and CDK-5 mediated phosphorylation of LIN-10, but not the hypoxia-inducible transcription factor HIF-1. Eun Chan Park, Christopher Rongo and colleagues The EMBO Journal (2012), 31, 1379 - 1393; 10.1038/emboj.2011.499 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 17 January 2012 Subject Categories: Membranes and Transport | Signal Transduction | Neuroscience O-GlcNAcylation of TAB1 modulates TAK1-mediated cytokine release The protein kinase TAK1 plays an important role in pro-inflammatory cytokine signalling. Interleukin-1- and osmotic stress-induced O-GlcNAcylation of its regulatory subunit TAB1 is required for full TAK1 activation to induce downstream cytokine production, linking this protein modification to innate immunity signalling. Shalini Pathak, Daan MF van Aalten and colleagues The EMBO Journal (2012), 31, 1394 - 1404; 10.1038/emboj.2012.8 Abstract | Full text | PDF | Supp. info. Published online: 03 February 2012 Subject Categories: Signal Transduction | Immunology DNA methylation profiling identifies epigenetic dysregulation in pancreatic islets from type 2 diabetic patients The first genome-scale DNA methylation study on pancreatic islets from type 2 diabetic patients identifies disease-associated DNA methylation pattern that translate into aberrant gene expression in novel factors relevant for β-cell function and survival. Michael Volkmar, François Fuks and colleagues The EMBO Journal (2012), 31, 1405 - 1426; 10.1038/emboj.2011.503 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 31 January 2012 Subject Categories: Chromatin and Transcription | Molecular Biology of Disease | Genomic and Computational Biology PML regulates PER2 nuclear localization and circadian function PER2 is a central clock component whose periodical nuclear localization mediates the BMAL1/CLOCK circadian transcriptional program. The tumour suppressor PML is a novel mammalian clock control gene: PML is deacetylated by SIRT1 inducing its interaction with PER2 and its nuclear translocation. Takao Miki, Kun-Sang Chang and colleagues The EMBO Journal (2012), 31, 1427 - 1439; 10.1038/emboj.2012.1 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 24 January 2012 Subject Categories: Chromatin and Transcription | Cellular Metabolism PP4 dephosphorylates Maf1 to couple multiple stress conditions to RNA polymerase III repression Maf1 represses RNA polymerase III transcription during stress and nutrient deprivation. Transcriptional repression upon stress is mediated by Maf1 dephosphorylation by nuclear protein phosphatase 4. Andrew J Oler and Bradley R Cairns The EMBO Journal (2012), 31, 1440 - 1452; 10.1038/emboj.2011.501 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 14 February 2012 Subject Categories: Chromatin and Transcription A role for α-adducin (ADD-1) in nematode and human memory ADD-1 regulates short- and long-term memory in C. elegans and is associated with episodic memory in human. ADD-1 stabilizes actin filaments, regulating synaptic plasticity and maintaining synaptic glutamate receptors during learning. Vanja Vukojevic, Attila Stetak and colleagues The EMBO Journal (2012), 31, 1453 - 1466; 10.1038/emboj.2012.14 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 03 February 2012 Subject Categories: Neuroscience Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex Shugoshin proteins mainly function to protect centromeric cohesion during cell division. Identification of the second frog shugoshin paralogue reveals that XSgo2 instead controls mitotic spindle assembly and chromosome alignment, via activation of Aurora B kinase and the kinesin MCAK. Teresa Rivera, Ana Losada and colleagues The EMBO Journal (2012), 31, 1467 - 1479; 10.1038/emboj.2012.4 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 24 January 2012 Subject Categories: Cell and Tissue Architecture | Cell Cycle Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes The transcription factor E2FA stimulates mutually exclusive plant cell-cycle states, mitotic proliferation and differentiation-associated endocycles. In complex with retinoblastoma protein RBR1, E2FA first maintains proliferation by repressing differentiation genes, while later acting independently to promote endocycle entry. Zoltán Magyar, László Bögre and colleagues The EMBO Journal (2012), 31, 1480 - 1493; 10.1038/emboj.2012.13 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 03 February 2012 Subject Categories: Cell Cycle | Plant Biology Myeloid translocation gene 16 is required for maintenance of haematopoietic stem cell quiescence In adult mice, haematopoietic stem cells are maintained in a quiescent state. The transcriptional repressor Myeloid Translocation Gene 16 (MTG16) is shown to govern this process. Melissa A Fischer, Scott W Hiebert and colleagues The EMBO Journal (2012), 31, 1494 - 1505; 10.1038/emboj.2011.500 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 20 January 2012 Subject Categories: Development | Differentiation and Death The architecture of functional modules in the Hsp90 co-chaperone Sti1/Hop The co-chaperone Sti1/Hop is required for the transfer of proteins from the Hsp70 to the Hsp90 chaperone system in eukaryotes. Structural and functional data elucidate how Sti1 regulates the ATPase cycle of Hsp90 to enable it to take over clients from Hsp70–Sti1. Andreas B Schmid, Johannes Buchner and colleagues The EMBO Journal (2012), 31, 1506 - 1517; 10.1038/emboj.2011.472 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 06 January 2012 Subject Categories: Proteins | Structural Biology Dynamics of the regulation of Hsp90 by the co-chaperone Sti1 Loading of proteins on the Hsp90 chaperone requires transfer from Hsp70 mediated by the co-chaperone Sti1/Hop. Biochemical and biophysical data show how Sti1 inhibits the ATPase activity of Hsp90 and enhances client transfer. Chung-Tien Lee, Matthias P Mayer and colleagues The EMBO Journal (2012), 31, 1518 - 1528; 10.1038/emboj.2012.37 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 21 February 2012 Subject Categories: Proteins | Structural Biology The active ClpP protease from M. tuberculosis is a complex composed of a heptameric ClpP1 and a ClpP2 ring Different from other bacteria, formation of the ATP-dependent ClpP protease in Mycobacterium requires two independently expressed ClpP1/2 subunits. Their co-assembly allows proteolytic activity to be stimulated by the ClpC1 regulatory ATPase and by N-blocked dipeptides newly identified as small molecule agonists, offering potential new tuberculosis drug targets. Tatos Akopian, Alfred L Goldberg and colleagues The EMBO Journal (2012), 31, 1529 - 1541; 10.1038/emboj.2012.5 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 27 January 2012 Subject Categories: Proteins | Microbiology and Pathogens Soj/ParA stalls DNA replication by inhibiting helix formation of the initiator protein DnaA The Bacillus subtilis ParA ATPase Soj affects chromosome segregation by controlling replication initiation. New in vitro and in vivo data show that Soj acts by blocking superhelical oligomerization of DnaA, a known prerequisite for DNA duplex remodelling and opening at bacterial replication origins. Graham Scholefield, Jeff Errington and Heath Murray The EMBO Journal (2012), 31, 1542 - 1555; 10.1038/emboj.2012.6 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 27 January 2012 Subject Categories: Genome Stability and Dynamics Coordination of multiple enzyme activities by a single PCNA in archaeal Okazaki fragment maturation In vitro reconstitution of Okazaki fragment processing shows that DNA polymerase, flap endonuclease and DNA ligase need to simultaneously bind to the same PCNA-sliding clamp molecule during DNA lagging strand replication. Thomas R Beattie and Stephen D Bell The EMBO Journal (2012), 31, 1556 - 1567; 10.1038/emboj.2012.12 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 03 February 2012 Subject Categories: Genome Stability and Dynamics Insights into Chi recognition from the structure of an AddAB-type helicase–nuclease complex Homologous recombination DNA repair requires double-strand break resection by helicase–nuclease enzymes. The crystal structure of bacterial AddAB in complex with DNA substrates shows that it employs an inactive helicase site to recognize ‘Chi’ recombination hotspot sequences that regulate resection. Kayarat Saikrishnan, Dale B Wigley and colleagues The EMBO Journal (2012), 31, 1568 - 1578; 10.1038/emboj.2012.9 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 03 February 2012 Subject Categories: Genome Stability and Dynamics Structure and assembly of the SF3a splicing factor complex of U2 snRNP SF3a regulates the assembly of the spliceosome. The crystal structure of the functional SF3a core subunits Prp21, Prp9 and Prp11 reveals specific binding to the stem-loop IIa of U2 snRNA during the assembly of the U2 snRNP. Pei-Chun Lin and Rui-Ming Xu The EMBO Journal (2012), 31, 1579 - 1590; 10.1038/emboj.2012.7 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 07 February 2012 Subject Categories: RNA | Structural Biology Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre The nineteen complex plays an important role in regulating in regulating spliceosome conformation and fidelity. Cwc2, a constituent of the complex, is located at the vicinity of the catalytic centre of the spliceosome and is required for the first step of pre-mRNA splicing, but not spliceosome assembly, by positioning the pre-mRNA and spliceosomal RNA. Nicolas Rasche, Reinhard Lührmann and colleagues The EMBO Journal (2012), 31, 1591 - 1604; 10.1038/emboj.2011.502 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 January 2012 Subject Categories: RNA Architecture and nucleic acids recognition mechanism of the THO complex, an mRNP assembly factor The THO complex integrates gene expression processes including transcription elongation, mRNA splicing, and export. The first structure of the complex reveals the positions of the subunits and maps nucleic acid binding. Álvaro Peña, Kamil Gewartowski, Andrzej Dziembowski and colleagues The EMBO Journal (2012), 31, 1605 - 1616; 10.1038/emboj.2012.10 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 07 February 2012 Subject Categories: RNA | Structural Biology Corrigenda Top Androgen receptor driven transcription in molecular apocrine breast cancer is mediated by FoxA1 Jessica L L Robinson, Jason S Carroll and colleagues The EMBO Journal (2012), 31, 1617; 10.1038/emboj.2012.59 Abstract | Full text | PDF Published online: 21 March 2012 Hypoxia regulates glutamate receptor trafficking through an HIF-independent mechanism Eun Chan Park, Christopher Rongo and colleagues The EMBO Journal (2012), 31, 1618 - 1619; 10.1038/emboj.2012.44 Abstract | Full text | PDF Published online: 10 February 2012 Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to The EMBO Journal. In order to do so, please purchase a subscription. 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