Laboratory Investigation - Table of Contents alert Volume 92 Issue 1

TABLE OF CONTENTS Volume 92, Issue 1 (January 2012) In this issue Inside LI Pathobiology in Focus Research Articles Also new AOP Sign up for e-alerts Recommend to your library Web feed Subscribe Advertisement Announcing Open Laboratory Investigation now offers authors the option to publish their articles with immediate open access upon publication. Open access articles will also be deposited on PubMed Central at the time of publication and will be freely available immediately. Find out more from the FAQs page.   Inside LI Top Inside Lab Invest 2012 92: 2-3; 10.1038/labinvest.2011.197 Full Text Pathobiology in Focus Top Investigative pathology: leading the post-genomic revolution Molecular pathologists have the rare ability to wed genomic tools with diagnostic skills and tissue-based pathology techniques for integrated diagnosis of human disease. However, the number of pathologists with expertise in genome-based research is relatively low. This situation must be rectified so that pathology can guide patient care in the post-genomic era. David M Berman, Marcus W Bosenberg, Robin L Orwant, Beth L Thurberg, Gulio F Draetta, Christopher DM Fletcher and Massimo Loda 2012 92: 4-8; advance online publication, October 10, 2011; 10.1038/labinvest.2011.147 Abstract | Full Text Research Articles Top ORAL AND GASTROINTESTINAL SYSTEMS Early endothelial damage and increased colonic vascular permeability in the development of experimental ulcerative colitis in rats and mice In ulcerative colitis, endothelial damage, increased colonic vascular permeability, perivascular edema and epithelial hypoxia precede epithelial barrier dysfunction, leading to erosions, ulceration and inflammation. These findings contribute to a better understanding of the cellular/molecular mechanisms in ulcerative colitis pathogenesis, and provide new targets for treatment and prevention. Ganna Tolstanova, Xiaoming Deng, Samuel W French, William Lungo, Brankica Paunovic, Tetyana Khomenko, Amrita Ahluwalia, Tessa Kaplan, Maria Dacosta-Iyer, Andrzej Tarnawski, Sandor Szabo and Zsuzsanna Sandor 2012 92: 9-21; advance online publication, September 5, 2011; 10.1038/labinvest.2011.122 Abstract | Full Text Acid modulates the squamous epithelial barrier function by modulating the localization of claudins in the superficial layers An in vitro stratified squamous epithelial cell-like culture system is used to show that tight junctions are located in the superficial layers of the esophagus; and that acid stimulation disrupts barrier function, at least in part, by modulating the amount and localization of claudin-4 in the superficial layers. Tadayuki Oshima, Junichi Koseki, Xin Chen, Takayuki Matsumoto and Hiroto Miwa 2012 92: 22-31; advance online publication, September 12, 2011; 10.1038/labinvest.2011.139 Abstract | Full Text GENITOURINARY AND REPRODUCTIVE SYSTEMS HSP27/HSPB1 as an adaptive podocyte antiapoptotic protein activated by high glucose and angiotensin II Podocyte heat-shock protein 27 (HSPB1) is increased in diabetic nephropathy and focal segmental glomerulosclerosis. HSPB1 siRNA targeting increases apoptosis of podocytes sensitized to angiotensin II or in a high-glucose milieu. HSPB1 upregulation therefore allows podocytes to better withstand an adverse highglucose or angiotensin II-rich environment, such as found in diabetic nephropathy. Maria D Sanchez-Niño, Ana B Sanz, Elsa Sanchez-Lopez, Marta Ruiz-Ortega, Alberto Benito-Martin, Moin A Saleem, Peter W Mathieson, Sergio Mezzano, Jesus Egido and Alberto Ortiz 2012 92: 32-45; advance online publication, September 19, 2011; 10.1038/labinvest.2011.138 Abstract | Full Text Microvascular density as an independent predictor of clinical outcome in renal cell carcinoma: an automated image analysis study The first automated digital assessment of microvascular density (MVD) in renal cell carcinoma using whole slide scanning is reported, including data normalization by analysis of normal tissue and calculation of the tumor/normal ratio. Increased MVD is an independent marker of tumor aggression, and higher adjusted MVD is associated with shorter disease free survival. Vladimir V Iakovlev, Manal Gabril, William Dubinski, Andreas Scorilas, Youssef M Youssef, Hala Faragalla, Kalman Kovacs, Fabio Rotondo, Shereen Metias, Androu Arsanious, Anna Plotkin, Andrew H F Girgis, Catherine J Streutker and George M Yousef 2012 92: 46-56; advance online publication, October 31, 2011; 10.1038/labinvest.2011.153 Abstract | Full Text HEPATIC AND PANCREATIC SYSTEMS Fascin, a novel marker of human hepatic stellate cells, may regulate their proliferation, migration, and collagen gene expression through the FAK-PI3K-Akt pathway Fascin, an actin-bundling protein, is shown to be a functionally significant marker of human hepatic stellate cells (HSC). Depletion of fascin in a cell line derived from HSC decreases cell migration, reduces cell survival, downregulates collagen and MMP production, and reduces the phosphorylation of focal adhesion kinase and Akt. Naoki Uyama, Yuji Iimuro, Norifumi Kawada, Hendrik Reynaert, Kazuhiro Suzumura, Tadamichi Hirano, Nobukazu Kuroda and Jiro Fujimoto 2012 92: 57-71; advance online publication, October 17, 2011; 10.1038/labinvest.2011.150 Abstract | Full Text Participation of liver progenitor cells in liver regeneration: lack of evidence in the AAF/PH rat model Liver progenitor cells (LPC) are capable of differentiating into hepatocytes when the normal regenerative response is overwhelmed or deficient. The contribution of LPC to liver cell replacement was measured in the 2-acetaminofluorene/partial hepatectomy rat model. Recovery of liver function was ensured by the proliferation of mature hepatocytes rather than LPCs. Ange-Clarisse Dusabineza, Noémi K Van Hul, Jorge Abarca-Quinones, Peter Starkel, Mustapha Najimi and Isabelle A Leclercq 2012 92: 72-81; advance online publication, September 12, 2011; 10.1038/labinvest.2011.136 Abstract | Full Text Reduced CaM/FLIP binding by a single point mutation in c-FLIPL modulates Fas-mediated apoptosis and decreases tumorigenesis Calmodulin (CaM) binds to c-FLIPL in a Ca2+-dependent manner. Deletion of the CaM-binding region increases Fas-mediated apoptosis and decreases tumorigenesis of cholangiocarcinoma cells. The H204 residue is responsible for c-FLIPL binding to CaM, which mediates the anti-apoptotic function of c-FLIPL through recruitment of caspase 8 into the death-inducing signaling complex. Gu Jing, Kaiyu Yuan, Qiuli Liang, Yong Sun, Xia Mao, Jay M McDonald and Yabing Chen 2012 92: 82-90; advance online publication, September 12, 2011; 10.1038/labinvest.2011.131 Abstract | Full Text ENDOCRINE, VISUAL AND AUDITORY SYSTEMS Bone marrow-derived monocyte lineage cells recruited by MIP-1β promote physiological revascularization in mouse model of oxygen-induced retinopathy Bone marrow-derived cells of monocyte lineage recruited by MIP-1β promote physiological revascularization of hypoxic avascular retinas. Promoting revascularization during the early phase of ischemic retinopathy may have therapeutic value in preventing vessel loss and retinal hypoxia, suppressing subsequent pathological neovascularization in hypoxia-induced visionthreatening retinal diseases. Keijiro Ishikawa, Shigeo Yoshida, Shintaro Nakao, Yukio Sassa, Ryo Asato, Riichiro Kohno, Mitsuru Arima, Takeshi Kita, Ayako Yoshida, Kenoki Ohuchida and Tatsuro Ishibashi 2012 92: 91-101; advance online publication, September 12, 2011; 10.1038/labinvest.2011.141 Abstract | Full Text Vision preservation during retinal inflammation by anthocyanin-rich bilberry extract: cellular and molecular mechanism The anthocyanin-rich bilberry extract has a neuroprotective effect on the mouse retina in an endotoxin-induced uveitis model. In addition to its anti-inflammatory effect that is mediated through IL-6 and STAT3, the bilberry extract ameliorates the intracellular elevation of reactive oxygen species in the inflamed retina. Seiji Miyake, Noriko Takahashi, Mariko Sasaki, Saori Kobayashi, Kazuo Tsubota and Yoko Ozawa 2012 92: 102-109; advance online publication, September 5, 2011; 10.1038/labinvest.2011.132 Abstract | Full Text ANGIOGENESIS, CARDIOVASCULAR AND PULMONARY SYSTEMS Loss of Arnt (Hif1β) in mouse epidermis triggers dermal angiogenesis, blood vessel dilation and clotting defects A novel Arnt-dependent paracrine signalling pathway links epidermal HIF activity and dermal vascular homeostasis. Given the role of Arnt in the adaptive response and the similarity of the reported phenotype with vascular features of tumor stroma and psoriatic skin, this pathway may serve as an interface between environmental stress and skin pathology. Assefa Wondimu, Lynda Weir, Douglas Robertson, Alexandre Mezentsev, Sergey Kalachikov and Andrey A Panteleyev 2012 92: 110-124; advance online publication, September 26, 2011; 10.1038/labinvest.2011.134 Abstract | Full Text The mitochondrial respiratory chain has a critical role in the antiviral process in Coxsackievirus B3-induced myocarditis Elimination of myocardial Coxsackievirus B3 (CVB3) is related to a cytokineregulated imbalance in mitochondrial oxidative phosphorylation, oxidative stress and apoptosis in infected murine hearts. Consequently, the regulation of energy metabolism may be of prognostic and of therapeutic significance for patients with CVB3-induced myocarditis. Linda Ebermann, Sylwia Wika, Inga Klumpe, Elke Hammer, Karin Klingel, Dirk Lassner, Uwe Völker, Ulrike Erben, Heinz Zeichhardt, Heinz-Peter Schultheiss and Andrea Dörner 2012 92: 125-134; advance online publication, October 3, 2011; 10.1038/labinvest.2011.145 Abstract | Full Text MODELS AND TECHNIQUES Liver precursor cells increase hepatic fibrosis induced by chronic carbon tetrachloride intoxication in rats In this new model of fibrosis using combined chronic acetylaminofluorene and CCl4 treatment, liver progenitor cells arising in portal areas and extending into parenchyma drive a fibrogenic response through TGF-β synthesis. This leads to liver fibrosis with porto-central septa progressing to cirrhosis, recapitulating the events observed in human chronic liver diseases. Marie-Noële Chobert, Dominique Couchie, Agnès Fourcot, Elie-Serge Zafrani, Yannick Laperche, Philippe Mavier and Arthur Brouillet 2012 92: 135-150; advance online publication, September 26, 2011; 10.1038/labinvest.2011.143 Abstract | Full Text Tumor-secreted SDF-1 promotes glioma invasiveness and TAM tropism toward hypoxia in a murine astrocytoma model A new murine analplastic astrocytoma cell line, ALTS1C1, recapitulates the histopathological of human high-grade glioma. In vivo, stromal-derived factor-1 produced by the cells decreases microvascular density and increases the number of hypoxic areas and supports aggregation of tumor-associated macrophages into areas of hypoxia and tumor invasiveness. Shu-Chi Wang, Ji-Hong Hong, Chuen Hsueh and Chi-Shiun Chiang 2012 92: 151-162; advance online publication, September 5, 2011; 10.1038/labinvest.2011.128 Abstract | Full Text Advertisement Ensure your access If you are unable to access the articles in this alert, your library may not subscribe to this journal. Our online recommendation form is a simple way to make your librarian aware that this journal is valuable to your research. Recommend now!   Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to Laboratory Investigation. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/nams/svc/myaccount (You will need to log in to be recognised as a nature.com registrant). 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