The EMBO Journal - Table of Contents alert Volume 30 Issue 24

TABLE OF CONTENTS 14 December 2011 | Volume 30, Issue 24 Have you seen? Articles Editor's Note Corrigendum Sign up for e-alerts Recommend to your library Web feed Subscribe Have you seen? Top Human pluripotent stem cells decouple respiration from energy production In human pluripotent stem cells, uncoupling protein 2 switches energy metabolism from respiration to glycolysis preventing their premature differentiation. Ng Shyh-Chang, Yuxiang Zheng, Jason W Locasale and Lewis C Cantley The EMBO Journal (2011), 30, 4851 - 4852; 10.1038/emboj.2011.436 Abstract | Full text | PDF Published online: 14 December 2011 Building a double hexamer of DNA helicase at eukaryotic replication origins Yeast Cdt1 multitasks in recruitment, loading and activation of the Mcm2-7 helicase at replication origins—including a key role in bringing two helicases to a single origin recognition complex Karim Labib The EMBO Journal (2011), 30, 4853 - 4855; 10.1038/emboj.2011.437 Abstract | Full text | PDF Published online: 14 December 2011 Rotate into shape: MreB and bacterial morphogenesis Several recent papers uncover interactions between the bacterial actin homologue, MreB, and components of the cell-wall synthesis machinery, revealing a tight interplay between cytoskeletal dynamics and cell-wall deposition. Sven van Teeffelen and Zemer Gitai The EMBO Journal (2011), 30, 4856 - 4857; 10.1038/emboj.2011.430 Abstract | Full text | PDF Published online: 14 December 2011 The unmasking of novel unipotent stem cells in the mammary gland Employing genetic lineage-tracing studies reveal the existence of novel, unipotent stem cells that serve the normal homeostasis of postnatal mammary epithelium. These findings add surprising complexity to the established stem cell hierarchy in the mammary epithelium. Jane E Visvader and Geoffrey J Lindeman The EMBO Journal (2011), 30, 4858 - 4859; 10.1038/emboj.2011.415 Abstract | Full text | PDF Published online: 08 November 2011 Articles Top UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells While studying metabolic fluxes in human pluripotent stem cells, this paper reveals UCP2 as metabolic switch from glycolysis to OXPHOS, facilitating early differentiation events. Jin Zhang, Ivan Khvorostov, Jason S Hong, Yavuz Oktay, Laurent Vergnes, Esther Nuebel, Paulin N Wahjudi, Kiyoko Setoguchi, Geng Wang, Anna Do, Hea-Jin Jung, J Michael McCaffery, Irwin J Kurland, Karen Reue, Wai-Nang P Lee, Carla M Koehler and Michael A Teitell The EMBO Journal (2011), 30, 4860 - 4873; 10.1038/emboj.2011.401 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 15 November 2011 FGF signalling inhibits neural induction in human embryonic stem cells The role of FGF-signal inhibition in embryonic cell fate decisions remained controversial. This paper identifies OTX2 induction and downregulation of Oct4/Nanog as immediate downstream events that cooperate with Pax6 for efficient establishment of the neuroectodermal lineage. Boris Greber, Philippe Coulon, Miao Zhang, Sören Moritz, Stefan Frank, Arnoldo José Müller-Molina, Marcos J Araúzo-Bravo, Dong Wook Han, Hans-Christian Pape and Hans R Schöler The EMBO Journal (2011), 30, 4874 - 4884; 10.1038/emboj.2011.407 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 15 November 2011 Multiple Cdt1 molecules act at each origin to load replication-competent Mcm2–7 helicases Replication initiation requires back-to-back loading of two Mcm2–7 helicases at a single origin. The replication licensing factor Cdt1 contributes to several discrete steps in this process, namely origin recruitment, chromatin loading, and conformational activation of Mcm2–7. Thomas J Takara and Stephen P Bell The EMBO Journal (2011), 30, 4885 - 4896; 10.1038/emboj.2011.394 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 01 November 2011 Dpb11 coordinates Mec1 kinase activation with cell cycle-regulated Rad9 recruitment Cyclin-dependent kinase phosphorylation of the replication checkpoint mediator Rad9 controls its association with Dpb11, a key activator of the yeast ATR homologue Mec1, thus conferring cell-cycle dependence to checkpoint signalling. Boris Pfander and John F X Diffley The EMBO Journal (2011), 30, 4897 - 4907; 10.1038/emboj.2011.345 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 23 September 2011 Inhibition of autophagy by TAB2 and TAB3 Expanding on recent crosstalk between autophagy and the NF-κB pathway, TAB2 and TAB3 are characterized as novel modulators of Beclin 1. Autophagic stimulation releases constitutive inhibition of Beclin 1 by TAB2/3 to initiate efficient autophagosome biogenesis. Alfredo Criollo, Mireia Niso-Santano, Shoaib Ahmad Malik, Mickael Michaud, Eugenia Morselli, Guillermo Mariño, Sylvie Lachkar, Alexander V Arkhipenko, Francis Harper, Gérard Pierron, Jean-Christophe Rain, Jun Ninomiya-Tsuji, José M Fuentes, Sergio Lavandero, Lorenzo Galluzzi, Maria Chiara Maiuri and Guido Kroemer The EMBO Journal (2011), 30, 4908 - 4920; 10.1038/emboj.2011.413 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 11 November 2011 Regulation of p53 stability and function by the deubiquitinating enzyme USP42 An RNAi screen identifies USP42 as a ubiquitin-specific isopeptidase that stabilizes p53 under transient stress conditions, and selectively enhances p53 function in the induction of cell-cycle arrest. Andreas K Hock, Arnaud M Vigneron, Stephanie Carter, Robert L Ludwig and Karen H Vousden The EMBO Journal (2011), 30, 4921 - 4930; 10.1038/emboj.2011.419 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 15 November 2011 A widespread family of bacterial cell wall assembly proteins While most steps of the bacterial cell wall synthesis pathway are well characterized, the identity of the enzymes that attach anionic polymers to peptidoglycan has been elusive. The LCP proteins are here shown to be likely candidates for this missing link. Yoshikazu Kawai, Jon Marles-Wright, Robert M Cleverley, Robyn Emmins, Shu Ishikawa, Masayoshi Kuwano, Nadja Heinz, Nhat Khai Bui, Christopher N Hoyland, Naotake Ogasawara, Richard J Lewis, Waldemar Vollmer, Richard A Daniel and Jeff Errington The EMBO Journal (2011), 30, 4931 - 4941; 10.1038/emboj.2011.358 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 30 September 2011 Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry The yeast kinesin-5 Cin8 can move bi-directionally on mitotic spindles and in-vitro assays provide insights into the molecular basis underlying this directional switching. Adina Gerson-Gurwitz, Christina Thiede, Natalia Movshovich, Vladimir Fridman, Maria Podolskaya, Tsafi Danieli, Stefan Lakämper, Dieter R Klopfenstein, Christoph F Schmidt and Larisa Gheber The EMBO Journal (2011), 30, 4942 - 4954; 10.1038/emboj.2011.403 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 18 November 2011 Proper synaptic vesicle formation and neuronal network activity critically rely on syndapin I F-BAR domain proteins sense or induce membrane curvature and play a widespread role in intracellular trafficking events. This study characterizes syndapin I knockout mice at the neurological, cell biological and molecular levels and demonstrates that this F-BAR protein acts as membrane anchoring factor for dynamins during synaptic vesicle regeneration. Dennis Koch, Isabella Spiwoks-Becker, Victor Sabanov, Anne Sinning, Tamar Dugladze, Anne Stellmacher, Rashmi Ahuja, Julia Grimm, Susann Schüler, Anke Müller, Frank Angenstein, Tariq Ahmed, Alexander Diesler, Markus Moser, Susanne tom Dieck, Rainer Spessert, Tobias Maria Boeckers, Reinhard Fässler, Christian Andreas Hübner, Detlef Balschun, Tengis Gloveli, Michael Manfred Kessels and Britta Qualmann The EMBO Journal (2011), 30, 4955 - 4969; 10.1038/emboj.2011.339 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 16 September 2011 OCRL controls trafficking through early endosomes via PtdIns4,5P2-dependent regulation of endosomal actin Mutations in the PtdIns4,5P2 5-phosphatase OCRL cause the oculo-cerebro-renal Lowe syndrome. The molecular mechanisms underlying this condition involve defective early endosomal recycling of megalin, the receptor mediating protein reabsorption in the kidney. Mariella Vicinanza, Antonella Di Campli, Elena Polishchuk, Michele Santoro, Giuseppe Di Tullio, Anna Godi, Elena Levtchenko, Maria Giovanna De Leo, Roman Polishchuk, Lisette Sandoval, Maria-Paz Marzolo and Maria Antonietta De Matteis The EMBO Journal (2011), 30, 4970 - 4985; 10.1038/emboj.2011.354 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 04 October 2011 Guanylate kinase domains of the MAGUK family scaffold proteins as specific phospho-protein-binding modules Membrane-associated guanylate kinases (MAGUK) family proteins contain an inactive guanylate kinase (GK) domain, whose function has been elusive. Here, this domain is revealed as a new type of phospho-peptide-binding module, in which the GMP-binding site has evolved to accommodate phospho-serines or -threonines. Jinwei Zhu, Yuan Shang, Caihao Xia, Wenning Wang, Wenyu Wen and Mingjie Zhang The EMBO Journal (2011), 30, 4986 - 4997; 10.1038/emboj.2011.428 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 25 November 2011 PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro The mechanism by which miRNAs induce silencing of gene expression remains a matter of much debate. This study shows that maximal silencing is dependent on multiple concerted interactions in the context of the full-length GW182 protein rather than single protein–protein associations. Takashi Fukaya and Yukihide Tomari The EMBO Journal (2011), 30, 4998 - 5009; 10.1038/emboj.2011.426 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 25 November 2011 Structure of the Shq1–Cbf5–Nop10–Gar1 complex and implications for H/ACA RNP biogenesis and dyskeratosis congenita Shq1 is required for the biogenesis of eukaryotic H/ACA ribonucleoproteins (RNPs) involved in telomere maintenance and pseudo-uridylation of rRNA and snRNA. This study describes the crystal structure of Shq1 in complex with three H/ACA RNP proteins, suggesting that Shq1 acts as an assembly chaperone of immature RNPs. Shuang Li, Jingqi Duan, Dandan Li, Shoucai Ma and Keqiong Ye The EMBO Journal (2011), 30, 5010 - 5020; 10.1038/emboj.2011.427 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 25 November 2011 Editor's Note Top miR-605 joins p53 network to form a p53:miR-605:Mdm2 positive feedback loop in response to stress Jiening Xiao, Huixian Lin, Xiaobin Luo, Xiaoyan Luo and Zhiguo Wang The EMBO Journal (2011), 30, 5021; 10.1038/emboj.2011.463 Abstract | Full text | PDF Published online: 14 December 2011 Corrigendum Top Extracellular heat shock protein-90α: linking hypoxia to skin cell motility and wound healing Wei Li, Yong Li, Shengxi Guan, Jianhua Fan, Chieh-Fang Cheng, Alexandra M Bright, Cindi Chinn, Mei Chen and David T Woodley The EMBO Journal (2011), 30, 5022; 10.1038/emboj.2011.420 Abstract | Full text | PDF Published online: 14 December 2011 Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to The EMBO Journal. 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