TABLE OF CONTENTS
| 05 October 2011 | Volume 30, Issue 19 |  | | |  |  Have you seen?
Review
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Eppendorf Award Winner 2011
In 2011 the prize was awarded to Assistant Professor Suzan Rooijakkers, PhD, (University Medical Center Utrecht, Department of Medical Microbiology, Utrecht, The Netherlands). Find out more about her research here.
If you want to apply for the Eppendorf Award 2012, click here. | |
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www.appliedbiosystems.com/taqman | | | | | | | | | | | Have you seen? | Top | |  | | Rac1 activation comes full circle Precise spatiotemporal control of the Rac1 GTPase coordinates actin dynamics. A positive feedback loop involving the actin-binding protein coronin and the Rac regulators ArhGEF7, Pak1 and RhoGDI promotes Rac activity and actin polymerization at the cell cortex. Marc Symons The EMBO Journal (2011), 30, 3875 - 3877; 10.1038/emboj.2011.330 Abstract | Full text | PDF Published online: 05 October 2011 | | | | G4 DNA: at risk in the genome A study in this issue offers new evidence for G-quadruplex (G4) DNA structure formation in vivo and its contribution to genomic instability, and identifies specific cellular mechanisms to cope with such threats. Luther Davis and Nancy Maizels The EMBO Journal (2011), 30, 3878 - 3879; 10.1038/emboj.2011.342 Abstract | Full text | PDF Published online: 05 October 2011 | | | | von Willebrand factor folds into a bouquet The healing of blood vessel injury requires release of von Willebrand factor (vWF). New structures explain the pH-dependent transition of tightly packed to extended forms of multimeric vWF. Volker Gerke The EMBO Journal (2011), 30, 3880 - 3881; 10.1038/emboj.2011.321 Abstract | Full text | PDF Published online: 05 October 2011 | | | | Starting from scratch: de novo kinetochore assembly in vertebrates Recent studies in Cell and Nature shed light on how the kinetochore is recruited to centromeres during cell division, using elegant cell-based and in vitro assays to recapitulate kinetochore formation at artificial centromeres. Sarion R Bowers and Barbara G Mellone The EMBO Journal (2011), 30, 3882 - 3884; 10.1038/emboj.2011.329 Abstract | Full text | PDF Published online: 06 September 2011 | | Review | Top | | | | FOXA1: master of steroid receptor function in cancer This review highlights recent progress on the predominant role of FOXA1 in nuclear hormone receptor activity with an emphasis on human breast and prostate cancer. Michael A Augello, Theresa E Hickey and Karen E Knudsen The EMBO Journal (2011), 30, 3885 - 3894; 10.1038/emboj.2011.340 Abstract | Full text | PDF Published online: 20 September 2011 | | Articles | Top | | | | Calcium microdomains at the immunological synapse: how ORAI channels, mitochondria and calcium pumps generate local calcium signals for efficient T-cell activation T-cell polarization and the formation of the immunological synapse (IS) are essential for calcium-dependent T-cell activation. Here, the findings report on the interplay between ORAI channels, mitochondria and PMCA at the IS to generate local Ca2+ microdomains that contribute to NFAT activity and T-cell activation. Ariel Quintana, Mathias Pasche, Christian Junker, Dalia Al-Ansary, Heiko Rieger, Carsten Kummerow, Lucia Nuñez, Carlos Villalobos, Paul Meraner, Ute Becherer, Jens Rettig, Barbara A Niemeyer and Markus Hoth The EMBO Journal (2011), 30, 3895 - 3912; 10.1038/emboj.2011.289 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 16 August 2011 | | | | Coronin 1A promotes a cytoskeletal-based feedback loop that facilitates Rac1 translocation and activation The Rac1 GTPase promotes cortical actin accumulation. Coronin 1a and the Rac1 GEF ArhGEF7 bind polymerized actin and promote Rac1 activation, thus establishing a positive feedback circuit to regulate the localization and activity of Rac1. Antonio Castro-Castro, Virginia Ojeda, María Barreira, Vincent Sauzeau, Inmaculada Navarro-Lérida, Olivia Muriel, José R Couceiro, Felipe X Pimentel-Muíños, Miguel A del Pozo and Xosé R Bustelo The EMBO Journal (2011), 30, 3913 - 3927; 10.1038/emboj.2011.310 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 26 August 2011 | | | | The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization Most kinesins move directionally along microtubules, but MCAK instead depolymerizes them. This study analyses the ATPase cycle of MCAK, identifying unusual kinetic features that fit with its unconventional activity. Claire T Friel and Jonathon Howard The EMBO Journal (2011), 30, 3928 - 3939; 10.1038/emboj.2011.290 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 26 August 2011 | | | | Mechanism of transcriptional repression at a bacterial promoter by analysis of single molecules Colocalization of single-molecule spectroscopy is used to resolve the mechanism of transcriptional inhibition by the Lac repressor by showing that it binds to the promoter and prevents recruitment of RNA polymerase and open complex formation. Alvaro Sanchez, Melisa L Osborne, Larry J Friedman, Jane Kondev and Jeff Gelles The EMBO Journal (2011), 30, 3940 - 3946; 10.1038/emboj.2011.273 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 09 August 2011 | | | | H3K27 demethylation by JMJD3 at a poised enhancer of anti-apoptotic gene BCL2 determines ERα ligand dependency Oestrogen receptor α cooperates with a histone demethylase to erase repressive chromatin marks and activate transcription in oestrogen-dependent cells, a mechanism dispensable in oestrogen-independent cells due to PI3K-mediated histone methyltransferase inactivation. Amy Svotelis, Stéphanie Bianco, Jason Madore, Gabrielle Huppé, Alexei Nordell-Markovits, Anne-Marie Mes-Masson and Nicolas Gévry The EMBO Journal (2011), 30, 3947 - 3961; 10.1038/emboj.2011.284 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 12 August 2011 | | | | Dual role of FoxA1 in androgen receptor binding to chromatin, androgen signalling and prostate cancer Combining ChIP-seq, bioinformatic and functional data, this study addresses the genome-wide properties of the androgen receptor (AR). Modulation of the AR-pioneering factor FoxA1 results in a new picture distinguishing FoxA1-dependent from FoxA1-independent AR-genomic loci and the discovery of a third class that becomes reprogrammed by FoxA1 depletion. Biswajyoti Sahu, Marko Laakso, Kristian Ovaska, Tuomas Mirtti, Johan Lundin, Antti Rannikko, Anna Sankila, Juha-Pekka Turunen, Mikael Lundin, Juho Konsti, Tiina Vesterinen, Stig Nordling, Olli Kallioniemi, Sampsa Hautaniemi and Olli A Jänne The EMBO Journal (2011), 30, 3962 - 3976; 10.1038/emboj.2011.328 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 September 2011 | | | | A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors The piRNA pathway plays an important role in germline development. This systematic screen of TUDOR domain-containing proteins in Drosophila describes roles for Vreteno, Brother of Yb and Sister of Yb in the somatic and/or germline piRNA pathways. Dominik Handler, Daniel Olivieri, Maria Novatchkova, Franz Sebastian Gruber, Katharina Meixner, Karl Mechtler, Alexander Stark, Ravi Sachidanandam and Julius Brennecke The EMBO Journal (2011), 30, 3977 - 3993; 10.1038/emboj.2011.308 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 23 August 2011 | | | | Nucleophosmin deposition during mRNA 3′ end processing influences poly(A) tail length Nucleophosmin (NPM1) has been implicated in many nuclear activities involving DNA and RNA. Here, NPM1 is deposited on cellular mRNAs, interacts with the CPSF processing factor and has a role in regulating poly(A) tail length. Fumihiko Sagawa, Hend Ibrahim, Angela L Morrison, Carol J Wilusz and Jeffrey Wilusz The EMBO Journal (2011), 30, 3994 - 4005; 10.1038/emboj.2011.272 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 05 August 2011 | | | | A cluster of ribosome synthesis factors regulate pre-rRNA folding and 5.8S rRNA maturation by the Rat1 exonuclease Ribosome biogenesis involves a number of pre-rRNA cleavage events. This study characterizes the pre-rRNA interaction sites of the ribosome synthesis factors Cic1, Erb1, Nop7, Nop12, Nop15 and Nop4 and the 5′-exonuclease Rat1 and their role in 5.8S rRNA processing. Sander Granneman, Elisabeth Petfalski and David Tollervey The EMBO Journal (2011), 30, 4006 - 4019; 10.1038/emboj.2011.256 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 02 August 2011 | | | | Assembly of Saccharomyces cerevisiae 60S ribosomal subunits: role of factors required for 27S pre-rRNA processing The molecular mechanism of ribosome biogenesis is still ill-understood. This study characterizes 27SA3 pre-rRNA processing in detail. All known A3 processing factors associate with preribosomes well before their requirement and establish a neighbourhood that stabilizes functional preribosomes containing 27S pre-rRNAs. Aarti Sahasranaman, Jill Dembowski, John Strahler, Philip Andrews, Janine Maddock and John L Woolford Jr The EMBO Journal (2011), 30, 4020 - 4032; 10.1038/emboj.2011.338 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 16 September 2011 | | | | G-quadruplex-induced instability during leading-strand replication G-rich nucleic acid stretches can form secondary structures that require dedicated resolving helicases, making their in vivo occurrence likely to affect genome stability. A defined G-quadruplex-forming sequence indeed perturbs replication in yeast, in a striking orientation-dependent manner. Judith Lopes, Aurèle Piazza, Rodrigo Bermejo, Barry Kriegsman, Arianna Colosio, Marie-Paule Teulade-Fichou, Marco Foiani and Alain Nicolas The EMBO Journal (2011), 30, 4033 - 4046; 10.1038/emboj.2011.316 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 26 August 2011 | | | | Human UPF1 interacts with TPP1 and telomerase and sustains telomere leading-strand replication UPF1, a helicase/ATPase with roles in mRNA surveillance that is also found at human telomeres, directly interacts with telomerase and a shelterin component, and involved selectively in telomere leading-strand replication. Raghav Chawla, Sophie Redon, Christina Raftopoulou, Harry Wischnewski, Sarantis Gagos and Claus M Azzalin The EMBO Journal (2011), 30, 4047 - 4058; 10.1038/emboj.2011.280 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 09 August 2011 | | | | RUNX1 regulates the CD34 gene in haematopoietic stem cells by mediating interactions with a distal regulatory element This study reveals molecular details of RUNX1 function in haematopoiesis. Employing various in vivo genetic models and chromatin conformation capture (3C) assays in HSCs, RUNX1 is shown to regulate CD34 expression by looping of a newly characterized distal regulatory element to the core promoter. Elena Levantini, Sanghoon Lee, Hanna S Radomska, Christopher J Hetherington, Meritxell Alberich-Jorda, Giovanni Amabile, Pu Zhang, David A Gonzalez, Junyan Zhang, Daniela S Basseres, Nicola K Wilson, Steffen Koschmieder, Gang Huang, Dong-Er Zhang, Alexander K Ebralidze, Constanze Bonifer, Yutaka Okuno, Bertie Gottgens and Daniel G Tenen The EMBO Journal (2011), 30, 4059 - 4070; 10.1038/emboj.2011.285 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 26 August 2011 | | | | A hippocampal insulin-growth factor 2 pathway regulates the extinction of fear memories Contextual fear memory results in altered expression of Insulin-growth factor 2, IGF2, pathway components. Modulation of hippocampal IGF2 signalling affects the extinction of fear memories. Roberto Carlos Agis-Balboa, Dario Arcos-Diaz, Jessica Wittnam, Nambirajan Govindarajan, Kim Blom, Susanne Burkhardt, Ulla Haladyniak, Hope Yao Agbemenyah, Athanasios Zovoilis, Gabriella Salinas-Riester, Lennart Opitz, Farahnaz Sananbenesi and Andre Fischer The EMBO Journal (2011), 30, 4071 - 4083; 10.1038/emboj.2011.293 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 26 August 2011 | | | | Splicing factor hnRNPH drives an oncogenic splicing switch in gliomas This study reveals two alternative splicing events that contribute to the development of glioma. HnRNPH is shown to control production of a pro-survival splice variant of the death-domain adaptor protein IG20-MADD and the motility-enhancing isoform of the RON receptor tyrosine kinase. Clare V LeFave, Massimo Squatrito, Sandra Vorlova, Gina L Rocco, Cameron W Brennan, Eric C Holland, Ying-Xian Pan and Luca Cartegni The EMBO Journal (2011), 30, 4084 - 4097; 10.1038/emboj.2011.259 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 September 2011 | | | | A pH-regulated dimeric bouquet in the structure of von Willebrand factor Hemostatic protein VWF promotes platelet binding to site of vascular injury. This study shows how VWF dimers ‘zip up’ at the C-terminal domains to form a bouquet-like structure, allowing packing into Weibel–Palade bodies and rapid release for secretion. Yan-Feng Zhou, Edward T Eng, Noritaka Nishida, Chafen Lu, Thomas Walz and Timothy A Springer The EMBO Journal (2011), 30, 4098 - 4111; 10.1038/emboj.2011.297 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 19 August 2011 | | Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to The EMBO Journal. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/nams/svc/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department. For print subscription enquiries, please contact our subscription department. For other enquiries, please contact our customer feedback department. 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