Nature Cell Biology contents: August 2011 Volume 13 Number 8, pp 875 - 1010

Advertisement BD Matrigel Matrix. Cited in more than 6000 papers. BD Matrigel Matrix is certified LDEV-free, and is the most trusted and extensively published basement membrane matrix for good reason: Reliability. Try BD Matrigel Matrix or other BD recommended materials for 3D culture and save 15% on your order. BD Extracellular Matrices give you reliability, confidence and consistency. bdbiosciences.com/cellculture/ecm TABLE OF CONTENTS August 2011 Volume 13, Issue 8 Editorial Turning Points Review News and Views Research Highlights Articles Letters Erratum Recommend to your library Web feed Available online only Subscribe Advertisement PROSEEK-the 1µl "ELISA" Does your assay consume too much of your precious sample? Is long immunoassay development time slowing down your research? Try the new Proseek® kit, use only 1 µl of plasma or serum sample per assay, no optimization required, and no wash steps. Learn more www.olink.com Advertisement Announcing Principles of Biology, an interactive textbook from Nature Publishing Group Principles of Biology is a peer-reviewed, affordable textbook solution for university-level Introductory Biology courses, featuring 200+ customizable learning modules, 175+ interactive exercises, 3,000+ assessment questions, and access from laptops, smartphones, or tablets. Learn more.   Editorial Top Change the Equation: Improving science and mathematics education in the US p875 doi:10.1038/ncb2318 Change the Equation, a non-profit group of more than 100 corporate organizations, is committed to improving the state of mathematics and science education in the US. Full Text | PDF Turning Points Top Hunting the elusive oncogene: a stroke of good luck p876 Robert A. Weinberg doi:10.1038/ncb2302 Full Text | PDF Review Top The Hippo pathway in organ size control, tissue regeneration and stem cell self-renewal pp877 - 883 Bin Zhao, Karen Tumaneng and Kun-Liang Guan doi:10.1038/ncb2303 Abstract | Full Text | PDF News and Views Top The SNXy flavours of endosomal sorting pp884 - 886 Ludger Johannes and Christian Wunder doi:10.1038/ncb2300 The retromer complex coordinates retrograde transport of cargo proteins between endosomes and the trans-Golgi network. The sorting nexin SNX3 is required for the retrograde trafficking of Wntless, but not of other retrograde cargo proteins, revealing that the cargo specificity of retromer is provided by the sorting nexins. Full Text | PDF See also: Article by Harterink et al. esBAF safeguards Stat3 binding to maintain pluripotency pp886 - 888 Noa Novershtern and Jacob H. Hanna doi:10.1038/ncb2311 How the unique chromatin configuration of embryonic stem cells (ESCs) integrates inputs from exogenous stimuli to maintain pluripotency remains largely unknown. The ESC-specific ATP-dependent chromatin-remodelling (esBAF) complex maintains the accessibility of the target sites of Stat3, a leukaemia inhibitory factor (LIF) signalling effector, by preventing repressive localized polycomb-mediated trimethylation of Lys 27 of histone 3 (H3K27me3). Full Text | PDF See also: Article by Ho et al. FBXW5 controls centrosome number pp888 - 890 Julia Pagan and Michele Pagano doi:10.1038/ncb2312 Regulatory mechanisms to prevent centriole overduplication during the cell cycle are not completely understood. In this issue, FBXW5 is shown to control the degradation of the centriole assembly factor HsSAS-6. Moreover, the study proposes that FBXW5 is a substrate of both PLK4 and APC/C, two established regulators of centriole duplication. Full Text | PDF See also: Letter by Puklowski et al. Research Highlights Research highlights p891 doi:10.1038/ncb2317 Full Text | PDF Cell Biology JOBS of the week PhD in Biophysics / Cell Biology / Developmental Biology Karlsruhe Institute of Technology (KIT) Karlsruhe, Germany Postdoctoral Associate University of Florida - Department of Infectious Diseases & Pathology Gainesville, FL Career Development Fellow Medical Research Council Edinburgh, EH4 2XU, Britain Research Associate in Epidemiology (Cohort Retention and Participation) University of Bristol Bristol, United Kingdom Post doc position in Molecular Virology, Biochemistry, Molecular Biology Kansas University Medical Center Kansas, USA More Science jobs from Cell Biology EVENT The 5th International Workshop On Cell Regulations in Division and Arrest 23 - 27 October 2011 Okinawa, Japan More science events from Articles Top Mitotic internalization of planar cell polarity proteins preserves tissue polarity pp893 - 902 Danelle Devenport, Daniel Oristian, Evan Heller and Elaine Fuchs doi:10.1038/ncb2284 Planar cell polarity (PCP) directs the orientation of mammalian epithelial cells in the skin but it is unclear how polarity is preserved during division. A dileucine motif in the atypical cadherin Celsr1 is shown to trigger the endocytosis of PCP components in mitosis to ensure that they are distributed equally to daughter cells and recycled back to the plasma membrane after division. Abstract | Full Text | PDF esBAF facilitates pluripotency by conditioning the genome for LIF/STAT3 signalling and by regulating polycomb function pp903 - 913 Lena Ho, Erik L. Miller, Jehnna L. Ronan, Wen Qi Ho, Raja Jothi and Gerald R. Crabtree doi:10.1038/ncb2285 The STAT3 transcription factor maintains pluripotency by preventing differentiation. A Brg1-containing chromatin-remodelling complex, esBAF, is shown to direct STAT3 to its targets across the pluripotent genome. Deletion of Brg1 leads to binding of the polycomb complex and subsequent silencing of LIF/STAT targets. Unexpectedly, Brg1 also helps the polycomb complex silence Hox genes thereby further contributing to pluripotency. Abstract | Full Text | PDF See also: News and Views by Novershtern & Hanna A SNX3-dependent retromer pathway mediates retrograde transport of the Wnt sorting receptor Wntless and is required for Wnt secretion pp914 - 923 Martin Harterink, Fillip Port, Magdalena J. Lorenowicz, Ian J. McGough, Marie Silhankova, Marco C. Betist, Jan R. T. van Weering, Roy G. H. P. van Heesbeen, Teije C. Middelkoop, Konrad Basler, Peter J. Cullen and Hendrik C. Korswagen doi:10.1038/ncb2281 Wnt secretion is facilitated by retrograde trafficking of the Wnt receptor Wntless. Korswagen and colleagues now show that endosome-to-Golgi trafficking of Wntless depends on an alternative retromer pathway that contains SNX3 in place of the canonical retromer sorting nexins. Abstract | Full Text | PDF See also: News and Views by Johannes & Wunder Cdk1-phosphorylated CUEDC2 promotes spindle checkpoint inactivation and chromosomal instability pp924 - 933 Yan-Fei Gao, Teng Li, Yan Chang, Yu-Bo Wang, Wei-Na Zhang, Wei-Hua Li, Kun He, Rui Mu, Cheng Zhen, Jiang-Hong Man, Xin Pan, Tao Li, Liang Chen, Ming Yu, Bing Liang, Yuan Chen, Qing Xia, Tao Zhou, Wei-Li Gong, Ai-Ling Li, Hui-Yan Li and Xue-Min Zhang doi:10.1038/ncb2287 The ubiquitin ligase APC/C is required for the metaphase-to-anaphase transition in mitosis. Downstream of Cdk1, CUEDC2 is shown to activate the APC/C by mediating the release of the checkpoint protein Mad2 from APC/C. Abstract | Full Text | PDF N-WASP regulates the epithelial junctional actin cytoskeleton through a non-canonical post-nucleation pathway pp934 - 943 Eva M. Kovacs, Suzie Verma, Radiya G. Ali, Aparna Ratheesh, Nicholas A. Hamilton, Anna Akhmanova and Alpha S. Yap doi:10.1038/ncb2290 N-WASP activates Arp2/3-mediated actin nucleation. N-WASP is now shown to stabilize and organize the actin cytoskeleton at cell-cell junctions through its interaction with the actin-binding protein WIRE. Abstract | Full Text | PDF Variegated gene expression caused by cell-specific long-range DNA interactions pp944 - 951 Daan Noordermeer, Elzo de Wit, Petra Klous, Harmen van de Werken, Marieke Simonis, Melissa Lopez-Jones, Bert Eussen, Annelies de Klein, Robert H. Singer and Wouter de Laat doi:10.1038/ncb2278 How regulatory elements spatially interact with their target DNA sequences is unclear. The β-globin locus control region (LCR) is found to take part in interchromosomal interactions with a few genes controlled by shared transcription factors, and to transcriptionally upregulate β-globin mRNA in a subset of cells. Abstract | Full Text | PDF Letters Top Cytoskeletal polarity mediates localized induction of the heart progenitor lineage pp952 - 957 James Cooley, Stacia Whitaker, Sarah Sweeney, Scott Fraser and Brad Davidson doi:10.1038/ncb2291 Cardiac fate choices in Ciona intestinalis depend on the CDC42-mediated formation of invasive protrusions that probe for inductive signals in the environment. First paragraph | Full Text | PDF Mir193b-365 is essential for brown fat differentiation pp958 - 965 Lei Sun, Huangming Xie, Marcelo A. Mori, Ryan Alexander, Bingbing Yuan, Shilpa M. Hattangadi, Qingqing Liu, C. Ronald Kahn and Harvey F. Lodish doi:10.1038/ncb2286 PRDM16 is known to promote the differentiation of myoblastic progenitors to brown adipocytes. The miR-193b/365 microRNAs are shown to be induced by PRDM16 and promote brown fat differentiation, as well as block myogenesis. First paragraph | Full Text | PDF Cleavage of cohesin rings coordinates the separation of centrioles and chromatids pp966 - 972 Laura Schöckel, Martin Möckel, Bernd Mayer, Dominik Boos and Olaf Stemmann doi:10.1038/ncb2280 While separase is implicated in centrosome disjunction as well as in chromosome separation, its precise role at the centrosome has been uncertain. The proteolytic activity of separase is demonstrated to induce centriole disengagement through cleavage of cohesin. First paragraph | Full Text | PDF A role for CSLD3 during cell-wall synthesis in apical plasma membranes of tip-growing root-hair cells pp973 - 980 Sungjin Park, Amy L. Szumlanski, Fangwei Gu, Feng Guo and Erik Nielsen doi:10.1038/ncb2294 In plants, cell growth is dependent on modifications of the cell wall. In root hairs, cell wall synthesis during tip growth is found to be mediated by the activity of cellulose synthase (CESA)-like protein CSLD3, which is present in the polarized plasma membrane. First paragraph | Full Text | PDF Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis pp981 - 988 Daphné Dambournet, Mickael Machicoane, Laurent Chesneau, Martin Sachse, Murielle Rocancourt, Ahmed El Marjou, Etienne Formstecher, Rémi Salomon, Bruno Goud and Arnaud Echard doi:10.1038/ncb2279 The PtdIns(4,5)P2 5-phosphatase OCRL, mutated in Lowe syndrome, is implicated in trafficking and associates with Rab GTPases. OCRL function is now extended to cytokinesis, where it controls abscission of the intercellular bridge downstream of Rab35 through the local reduction of both PtdIns(4,5)P2 levels and actin accumulation. First paragraph | Full Text | PDF Lpd depletion reveals that SRF specifies radial versus tangential migration of pyramidal neurons pp989 - 995 Elaine M. Pinheiro, Zhigang Xie, Amy L. Norovich, Marina Vidaki, Li-Huei Tsai and Frank B. Gertler doi:10.1038/ncb2292 During brain development, pyramidal neurons migrate from the ventricular zone to reach their final destination in the cortex. In vivo depletion experiments shows that lamellipodin, through an effect on serum response factor, determines the neuronal migration mode in the developing cortex. First paragraph | Full Text | PDF COPI acts in both vesicular and tubular transport pp996 - 1003 Jia-Shu Yang, Carmen Valente, Roman S. Polishchuk, Gabriele Turacchio, Emilie Layre, D. Branch Moody, Christina C. Leslie, Michael H. Gelb, William J. Brown, Daniela Corda, Alberto Luini and Victor W. Hsu doi:10.1038/ncb2273 Hsu and colleagues show that COPI-coated buds are a common progenitor for both vesicles and tubules. The choice between these two carriers is mediated by the opposing activities of the acyltransferase LPAAT-γ and the phospholipase cPLA2-α. First paragraph | Full Text | PDF The SCF-FBXW5 E3-ubiquitin ligase is regulated by PLK4 and targets HsSAS-6 to control centrosome duplication pp1004 - 1009 Anja Puklowski, Yahya Homsi, Debora Keller, Martin May, Sangeeta Chauhan, Uta Kossatz, Viktor Grünwald, Stefan Kubicka, Andreas Pich, Michael P. Manns, Ingrid Hoffmann, Pierre Gönczy and Nisar P. Malek doi:10.1038/ncb2282 The PLK4 kinase and centrosomal protein HsSAS-6 both regulate centrosome duplication. PLK4 negatively controls an FBXW5-containing ubiquitin ligase, which targets SAS-6 for destruction to restrict centrosome re-duplication. First paragraph | Full Text | PDF See also: News and Views by Pagan & Pagano Erratum Top RasGRF suppresses Cdc42-mediated tumour cell movement, cytoskeletal dynamics and transformation p1010 Fernando Calvo, Victoria Sanz-Moreno, Lorena Agudo-Ibáñez, Fredrik Wallberg, Erik Sahai, Christopher J. Marshall and Piero Crespo doi:10.1038/ncb2319 Full Text | PDF Top Advertisement UCSD Clinical and Translational Research Institute and Nature Medicine present: Vascular Disease 2011: From bench to bedside October 13-15, 2011 • La Jolla, CA, USA This meeting will explore innovative approaches to bridge laboratory investigation and clinical research in disorders affecting the vasculature, including cancer, inflammatory disease and cardiovascular disease. For more information and to register visit: www.nature.com/natureconferences/b2b2011   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/myaccount (You will need to log in to be recognised as a nature.com registrant) For further technical assistance, please contact our registration department For print subscription enquiries, please contact our subscription department For other enquiries, please contact our customer feedback department Nature Publishing Group | 75 Varick Street, 9th Floor | New York | NY 10013-1917 | USA Nature Publishing Group's worldwide offices: London - Paris - Munich - New Delhi - Tokyo - Melbourne San Diego - San Francisco - Washington - New York - Boston Macmillan Publishers Limited is a company incorporated in England and Wales under company number 785998 and whose registered office is located at Brunel Road, Houndmills, Basingstoke, Hampshire RG21 6XS. © 2011 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.