The EMBO Journal Table of Contents for 02 May 2019; Vol. 38, No. 9

02 May 2019 | Volume 38, Number 9 Submit Table of Contents Commentary News & Views Articles Corrigendum Volume 38, Number 9 Commentary A supporting ecosystem to mature extracellular vesicles into clinical application Research on Extracellular Vesicles (EV) holds great potential for developing diagnostic and therapeutic applications for a wide range of diseases. The authors define eight pillars and propose minimal guidelines for good laboratory practice and to improve experimental reproducibility and reliability in EV research. Olivier De Wever and An Hendrix Published online 11.04.2019 News & Views Lining up for quality control: linear ubiquitin and proteotoxicity While linear ubiquitin is well known to play a role in inflammatory signaling, new work shows that it is also regulates stability and proteotoxicity of protein aggregates involved in neurodegenerative diseases. R Luke Wiseman Published online 11.04.2019 A choreography of intracellular Ca2+ and extracellular ATP to refine auditory nociceptors before hearing New work shows how coordinated Ca2+ waves in non‐sensory and sensory cells activate the nociception sensory system in the developing cochlea. Michael G Leitner and Dominik Oliver Published online 11.04.2019 Articles A protein quality control pathway regulated by linear ubiquitination Recruitment of the LUBAC E3 ligase, best known for its function in NF‐κB signalling, to protein aggregates for linear ubiquitin‐mediated removal of misfolded neurodegenerative disease proteins such as huntingtin, SOD1 or TDP‐43 exemplifies a new, potentially targetable cellular mechanism for controlling proteinopathies. Eva M van Well, Verian Bader, Maria Patra, Ana Sánchez‐Vicente, Jens Meschede, Nikolas Furthmann, Cathrin Schnack, Alina Blusch, Joseph Longworth, Elisabeth Petrasch‐Parwez, Kohji Mori, Thomas Arzberger, Dietrich Trümbach, Lena Angersbach, Cathrin Showkat, Dominik A Sehr, Lena A Berlemann, Petra Goldmann, Albrecht M Clement, Christian Behl, Andreas C Woerner, Carsten Saft, Wolfgang Wurst, Christian Haass, Gisa Ellrichmann, Ralf Gold, Gunnar Dittmar, Mark S Hipp, F Ulrich Hartl, Jörg Tatzelt and Konstanze F Winklhofer Published online 18.03.2019 Coordinated calcium signalling in cochlear sensory and non‐sensory cells refines afferent innervation of outer hair cells Spontaneous Ca2+ waves in non‐sensory cells increase and synchronize Ca2+ signalling in outer hair cells via ATP‐induced activation of P2X3 receptors, promoting functional differentiation and innervation of sensory cells. Federico Ceriani, Aenea Hendry, Jing‐Yi Jeng, Stuart L Johnson, Friederike Stephani, Jennifer Olt, Matthew C Holley, Fabio Mammano, Jutta Engel, Corné J Kros, Dwayne D Simmons and Walter Marcotti Published online 25.02.2019 Mutant Lef1 controls Gata6 in sebaceous gland development and cancer Genetic work combined with lineage tracing analyses reveals a link between mutant Lef1 and Gata6 in the formation of sebaceous glands and associated tumors. Bénédicte Oulès, Emanuel Rognoni, Esther Hoste, Georgina Goss, Ryan Fiehler, Ken Natsuga, Sven Quist, Remco Mentink, Giacomo Donati and Fiona M Watt Published online 18.03.2019 A slow transcription rate causes embryonic lethality and perturbs kinetic coupling of neuronal genes RNA polymerase II elongation rates play key roles in expression and alternative splicing patterns of genes, with notably higher impact in neurons differentiating from embryonic stem cells than in pluripotent cells. Magdalena M Maslon, Ulrich Braunschweig, Stuart Aitken, Abigail R Mann, Fiona Kilanowski, Chris J Hunter, Benjamin J Blencowe, Alberto R Kornblihtt, Ian R Adams and Javier F Cáceres Published online 15.04.2019 TRAF6 directs FOXP3 localization and facilitates regulatory T‐cell function through K63‐linked ubiquitination K63‐linked ubiquitination of FOXP3 by TRAF6 E3 ligase controls suppressive Treg functions, immune homeostasis and anti‐tumour immunity in mice. Xuhao Ni, Wei Kou, Jian Gu, Ping Wei, Xiao Wu, Hao Peng, Jinhui Tao, Wei Yan, Xiaoping Yang, Andriana Lebid, Benjamin V Park, Zuojia Chen, Yizhu Tian, Juan Fu, Stephanie Newman, Xiaoming Wang, Hongbin Shen, Bin Li, Bruce R. Blazar, Xuehao Wang, Joseph Barbi, Fan Pan and Ling Lu Published online 18.03.2019 Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly The X‐ray structures of IFT22/RabL5 reveal an unusual GTP/GDP‐binding mode and the structural basis for its interaction with the intraflagellar transport complex components IFT74/81 in regulation of flagellum formation in Trypanosoma. Stefanie Wachter, Jamin Jung, Shahaan Shafiq, Jerome Basquin, Cécile Fort, Philippe Bastin and Esben Lorentzen Published online 02.04.2019 Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy The core autophagy protein ATG16L1 binds phosphoinositides on pre‐autophagosomal structures via its coiled‐coil domain to promote LC3 lipidation and autophagosome maturation. Leo J Dudley, Ainara G Cabodevilla, Agata N Makar, Martin Sztacho, Tim Michelberger, Joseph A Marsh, Douglas R Houston, Sascha Martens, Xuejun Jiang and Noor Gammoh Published online 01.04.2019 ALYREF links 3′‐end processing to nuclear export of non‐polyadenylated mRNAs An RNA‐binding protein coupling nuclear export and 3′‐end processing of polyadenylated mRNAs exerts similar function on non‐polyadenylated replication‐dependent histone mRNAs. Jing Fan, Ke Wang, Xian Du, Jianshu Wang, Suli Chen, Yimin Wang, Min Shi, Li Zhang, Xudong Wu, Dinghai Zheng, Changshou Wang, Lantian Wang, Bin Tian, Guohui Li, Yu Zhou and Hong Cheng Published online 11.03.2019 Protein translocation by the SecA ATPase occurs by a power‐stroke mechanism Single‐molecule FRET reveals that the bacterial SecA translocase uses ATP hydrolysis to push a polypeptide segment into the SecY channel, and phosphate release to slide it through the SecA‐SecY complex. Marco A Catipovic, Benedikt W Bauer, Joseph J Loparo and Tom A Rapoport Published online 15.03.2019 Corrigendum The assembly of lipid droplets and their roles in challenged cells W Mike Henne, Michael L Reese and Joel M Goodman Published online 25.04.2019   Unsubscribe from or edit your subscription for this service. Copyright © 2019 by the European Molecular Biology Organisation.