The EMBO Journal Table of Contents for 01 April 2019; Vol. 38, No. 7

01 April 2019 | Volume 38, Number 7 Submit Table of Contents News & Views Articles Volume 38, Number 7 News & Views Bub1—the zombie protein that CRISPR cannot kill Two recent studies demonstrate potential pitfalls of inferring cellular functions of near‐essential proteins from “knockout” via CRISPR/Cas9 genome editing. Patrick Meraldi Published online 08.03.2019 Revealing chromatin organization in metaphase chromosomes Cryo‐electron tomography data revealing plate‐like layers of interdigitated nucleosomes on isolated human chromosomes suggest revised models for mitotic chromatin compaction. Beat Fierz Published online 04.03.2019 Articles Histidine is selectively required for the growth of Myc‐dependent dedifferentiation tumours in the Drosophila CNS Decreased dietary uptake of histidine differentially affects Drosophila brain cancer models, reducing pathological growth of only those neuronal clones that depend on Myc/eIF4E‐mediated cell size increases. Francesca Froldi, Panayotis Pachnis, Milán Szuperák, Olivia Costas, Tharindu Fernando, Alex P Gould and Louise Y Cheng Published online 25.02.2019 Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex Combination of genome editing and RNAi knockdown unveils that two separate pathways fulfill essential functions in kinetochore recruitment of mammalian checkpoint hub Mad1. Gang Zhang, Thomas Kruse, Claudia Guasch Boldú, Dimitriya H Garvanska, Fabian Coscia, Matthias Mann, Marin Barisic and Jakob Nilsson Published online 19.02.2019 Frozen‐hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure Cryo‐electron tomography of non‐crosslinked, non‐surface‐absorbed samples implicates stacked planar layers of mononucleosome width in 3D‐assembly of compact mitotic chromatin. Andrea Chicano, Eva Crosas, Joaquín Otón, Roberto Melero, Benjamin D Engel and Joan‐Ramon Daban Published online 04.01.2019 NBS1 promotes the endonuclease activity of the MRE11‐RAD50 complex by sensing CtIP phosphorylation In vitro reconstitution defines the crucial contribution of the accessory subunit in the mammalian MRN complex for end resection of DNA double‐strand breaks. Roopesh Anand, Arti Jasrotia, Diana Bundschuh, Sean Michael Howard, Lepakshi Ranjha, Manuel Stucki and Petr Cejka Published online 20.02.2019 Ssu72 phosphatase is a conserved telomere replication terminator Reversal of inhibitory CDK phosphorylation on its Stn1 subunit allows (C)ST complex recruitment for telomeric DNA fill‐in synthesis in both fission yeast and human cells. Jose Miguel Escandell, Edison SM Carvalho, Maria Gallo‐Fernandez, Clara C Reis, Samah Matmati, Inês Matias Luís, Isabel A Abreu, Stéphane Coulon and Miguel Godinho Ferreira Published online 21.02.2019 Hfq‐dependent mRNA unfolding promotes sRNA‐based inhibition of translation Different from its canonical role as an RNA interaction platform, the RNA chaperone Hfq regulates biofilm formation by remodeling dgcM mRNA, thus enabling its repression via OmrA/B sRNAs. Mirthe Hoekzema, Cédric Romilly, Erik Holmqvist and E Gerhart H Wagner Published online 04.03.2019 BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses New mouse models and genomic analyses reveal context‐dependent roles for BRD4 during normal hematopoiesis and inflammation. Anup Dey, Wenjing Yang, Anne Gegonne, Akira Nishiyama, Richard Pan, Ryoji Yagi, Alex Grinberg, Fred D Finkelman, Karl Pfeifer, Jinfang Zhu, Dinah Singer, Jun Zhu and Keiko Ozato Published online 06.03.2019 The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1 NANOG regulates the transition from mesoderm to primitive hematopoietic system by directly repressing erythroid lineage specification. Julio Sainz de Aja, Sergio Menchero, Isabel Rollan, Antonio Barral, Maria Tiana, Wajid Jawaid, Itziar Cossio, Alba Alvarez, Gonzalo Carreño‐Tarragona, Claudio Badia‐Careaga, Jennifer Nichols, Berthold Göttgens, Joan Isern and Miguel Manzanares Published online 27.02.2019 Extracellular matrix sensing by FERONIA and Leucine‐Rich Repeat Extensins controls vacuolar expansion during cellular elongation in Arabidopsis thaliana Extracellular Leucine‐Rich Repeat Extensin proteins link the plasma membrane receptor‐like kinase FERONIA with the cell wall for sensing mechanical constraints affecting vacuolar size and plant cell elongation. Kai Dünser, Shibu Gupta, Aline Herger, Mugurel I Feraru, Christoph Ringli and Jürgen Kleine‐Vehn Published online 08.03.2019   Unsubscribe from or edit your subscription for this service. Copyright © 2019 by the European Molecular Biology Organisation.