Nature Biotechnology Contents: October 2018, Volume 36 Issue 10

Advertisement Ready to unlock the potential of your favorite plant or animal genome? Submit your 250 word grant proposal for a chance to win PacBio DNA sequencing for de novo assembly or RNA sequencing of full-length transcripts. Apply by October 19 TABLE OF CONTENTS October 2018 Volume 36, Issue 10 Editorial News Opinion and Comment Feature News and Views Research Resource Amendments Careers and Recruitment Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement Bringing Edman Back to Life Shimadzu's protein sequencers provide reliable, sensitive N-terminal sequencing to researchers through automated Edman degradation. Software enables compliance with FDA 21 CFR Part 11 guidelines, while easy-to-use data analysis functions simplify operation, data processing and reporting. Learn more   Editorial In defense of the press... and more    p905 doi:10.1038/nbt.4280 The biotech community's decision to speak out in defense of freedom of the press is also a reaffirmation of core principles essential for the sector's success. News Sangamo's landmark genome editing trial gets mixed reception    pp907 - 908 Cormac Sheridan doi:10.1038/nbt1018-907 Setbacks shadow microRNA therapies in the clinic    pp909 - 910 Dan Jones doi:10.1038/nbt1018-909 Smart insulin: redesign could end hypoglycemia risk    pp911 - 912 Brian Owens doi:10.1038/nbt1018-911a Akcea's antisense drug rejection worries analysts    p911 doi:10.1038/nbt1018-911b Regeneron buys into CAR-Ts with Bluebird Bio deal    p912 doi:10.1038/nbt1018-912 FDA approves Galafold, a triumph for Amicus    p913 Nuala Moran doi:10.1038/nbt1018-913 US law threatens flow of foreign capital to biotech sector    pp914 - 915 Christopher Morrison doi:10.1038/nbt1018-914 Around the world in a month    p915 doi:10.1038/nbt1018-915a Correction    p915 doi:10.1038/nbt1018-915b News Feature The virome hunters    pp916 - 919 Charles Schmidt doi:10.1038/nbt.4268 Ambitious efforts to catalog viruses across the globe may facilitate our understanding of viral communities and ecology, boost infectious disease diagnostics and surveillance, and spur new therapeutics. Charles Schmidt investigates. Advertisement Searching for a new career? At Nature Research we frequently recruit talented individuals to join our editorial and publishing teams. If you have a PhD and a passion for science this may be the perfect career for you. Visit our research editorial and publishing careers website to discover more about what we do    Opinion and Comment Correspondence Biotech leaders call for free press    pp920 - 922 Ron Cohen, Steven Holtzman, Jeremy M Levin, John M Maraganore, the Signatories to the Statement et al. doi:10.1038/nbt.4271 Commentary Opening options for material transfer    pp923 - 927 Linda Kahl, Jennifer Molloy, Nicola Patron, Colette Matthewman, Jim Haseloff et al. doi:10.1038/nbt.4263 The Open Material Transfer Agreement is a material-transfer agreement that enables broader sharing and use of biological materials by biotechnology practitioners working within the practical realities of technology transfer. Are the new clinical trial transparency rules incompatible with the patentability requirements in Europe?    pp928 - 930 Lorenz Kallenbach & Marco K Vallazza doi:10.1038/nbt.4265 New transparency policies have created a tension between the regulatory obligation to post clinical trials early and the desire to obtain patent protection. Feature Patents Recent patents in antimicrobials    p931 doi:10.1038/nbt.4275 News and Views Decoding mood    pp932 - 933 doi:10.1038/nbt.4258 Invasive recordings of human brain activity predict mood fluctuations. Designing efficient translation    pp934 - 935 Thomas E Gorochowski & Tom Ellis doi:10.1038/nbt.4257 A massive study informed by Design of Experiments uncovers factors that boost protein synthesis. Research Highlights    p935 doi:10.1038/nbt.4270 Barcodes galore for developmental biology    p936 Markus Elsner doi:10.1038/nbt.4269 Research Perspective How user intelligence is improving PubMed    pp937 - 945 Nicolas Fiorini, Robert Leaman, David J Lipman & Zhiyong Lu doi:10.1038/nbt.4267 Machine learning and natural language processing methods are applied to learn from PubMed searches and improve user experience. Brief Communications Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion    pp946 - 949 Xiao Wang, Jianan Li, Ying Wang, Bei Yang, Jia Wei et al. doi:10.1038/nbt.4198 Increased efficiency of base editing in methylated DNA using human APOBEC3A as a deaminase. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A    pp950 - 953 Yuan Zong, Qianna Song, Chao Li, Shuai Jin, Dingbo Zhang et al. doi:10.1038/nbt.4261 A base editor containing a human cytidine deaminase offers a larger editing window. Articles Mood variations decoded from multi-site intracranial human brain activity    pp954 - 961 Omid G Sani, Yuxiao Yang, Morgan B Lee, Heather E Dawes, Edward F Chang et al. doi:10.1038/nbt.4200 Mood state changes are decoded using human neural activity data from electrodes implanted in seven epilepsy patients. Paired-cell sequencing enables spatial gene expression mapping of liver endothelial cells    pp962 - 970 Keren Bahar Halpern, Rom Shenhav, Hassan Massalha, Beata Toth, Adi Egozi et al. doi:10.1038/nbt.4231 The expression profile and tissue coordinates of liver endothelial cells are determined using a paired-cell sequencing approach that extracts spatial information from attached hepatocytes. Letters Conversion of staphylococcal pathogenicity islands to CRISPR-carrying antibacterial agents that cure infections in mice    pp971 - 976 doi:10.1038/nbt.4203 Engineered staphylococcal pathogenicity islands offer a potentially superior alternative to phages for antibacterial therapy An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities    pp977 - 982 Jason M Gehrke, Oliver Cervantes, M Kendell Clement, Yuxuan Wu, Jing Zeng et al. doi:10.1038/nbt.4199 The precision of base editing is enhanced with an engineered version of the APOBEC3A deaminase. A universal SNP and small-indel variant caller using deep neural networks    pp983 - 987 Ryan Poplin, Pi-Chuan Chang, David Alexander, Scott Schwartz, Thomas Colthurst et al. doi:10.1038/nbt.4235 DeepVariant uses convolutional neural networks to improve the accuracy of variant calling. On-demand manufacturing of clinical-quality biopharmaceuticals    pp988 - 995 Laura E Crowell, Amos E Lu, Kerry R Love, Alan Stockdale, Steven M Timmick et al. doi:10.1038/nbt.4262 A benchtop system for protein synthesis achieves purity and potency comparable to those of marketed drugs. Resources A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life    pp996 - 1004 Donovan H Parks, Maria Chuvochina, David W Waite, Christian Rinke, Adam Skarshewski et al. doi:10.1038/nbt.4229 Interpretation of microbial genome data will be improved by a fully revised bacterial taxonomy. Resource Evaluation of 244,000 synthetic sequences reveals design principles to optimize translation in Escherichia coli    pp1005 - 1015 Guillaume Cambray, Joao C Guimaraes & Adam Paul Arkin doi:10.1038/nbt.4238 Large and systematic mRNA library design disentangles the complex sequence determinants of translation efficiency in bacteria. Amendments Publisher Correction: Guided self-organization and cortical plate formation in human brain organoids    p1016 Madeline A Lancaster, Nina S Corsini, Simone Wolfinger, E Hilary Gustafson, Alex W Phillips et al. doi:10.1038/nbt1018-1016a Author Correction: Precision medicine in the clouds    p1016 Henrik Vogt, Sara Green & John Brodersen doi:10.1038/nbt1018-1016b Careers and Recruitment Gender disparities among independent fellows in biomedical research    pp1018 - 1021 Jason M Sheltzer doi:10.1038/nbt.4274 People People    p1022 doi:10.1038/nbt.4264 Advertisement Nature Briefing is an essential round-up of science news, opinion and analysis, free in your inbox every weekday. With Nature Briefing, we'll keep you updated on the latest research, so you can focus on yours. Click here to sign up.   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

This email has been sent to edgeblog.alerts.2011.001@gmail.com. If you no longer wish to receive the email alerts from Nature Biotechnology click here to unsubscribe . If you wish to discontinue all email services from Nature Research please click here to unsubscribe . For further technical assistance, please contact our registration department. For print subscription enquiries, please contact our subscription department. For other enquiries, please contact our customer feedback department. Macmillan Publishers Limited is a company incorporated in England & Wales under company number 785998 & whose registered office is located at The Campus, 4 Crinan Street, London, N1 9XW. Nature Research | One New York Plaza, Suite 4500 | New York | NY 10004-1562 | USA Nature is part of Springer Nature. © 2018 Springer Nature Limited. All rights reserved.