Nature Biotechnology Contents: Volume 34 pp 1077 - 1208

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Advertisement 2 Immuno-Oncology White Papers  How do advanced technology and key research tools like genomics, anatomic pathology and flow cytometry achieve better outcomes for your Immuno-Oncology development programs?  Download today and learn 4 key ways immuno-oncology clinical trials are being transformed. TABLE OF CONTENTS November 2016 Volume 34, Issue 11 In This Issue Editorials News Correction Bioentrepreneur Opinion and Comment Features News and Views Research Careers and Recruitment Advertisement   Sequence lung cancer-specific markers from liquid biopsies Archer®Reveal ctDNA™ 28 is a sensitive NGS-based assay to detect lung cancer-specific markers in plasma. Relevant gene targets include: EGFR, PIK3CA, KRAS, BRAF, ALK and ROS1 Get Reveal ctDNA 28 for lung cancer and other solid tumor markers. Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement Nature Outlook: Precision Medicine Health care that is tailored on the basis of an individual's genes, lifestyle or environment, is not a modern concept. But advances in genetics and the growing availability of health data for researchers and physicians promise to make this new era of medicine more personalized than ever before. Access the Outlook free online for six months Sponsored by: Illumina, Inc.      Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement In January 2017 Cell Press will take over publishing the Molecular Therapy family of journals. To keep receiving e-alerts for these titles, please visit http://www.cell.com/molecular-therapy-family/home   Advertisement In January 2017 Cell Press will take over publishing the Molecular Therapy family of journals. To keep receiving e-alerts for these titles, please visit http://www.cell.com/molecular-therapy-family/home   Focus on Single-cell Technology   Contents In This Issue Editorial News Feature News and Views Perspective Review     Single cell technologies are becoming increasingly important tools in biological analysis. Complementing average measurements on bulk populations of cells, single-cell measurements provide a finer-grained picture of complex biology and unmask heterogeneity that is present in tissues. With the increasing sophistication of microfluidics, electrophysiology measurements, high-resolution imaging, deep sequencing and mass spectrometry platforms, a more detailed picture of cellular subtype, physical location in tissue, and clonal evolution is emerging. Moreover, the exquisite sensitivity of these approaches is enabling the identification of rare cells of potential functional or pathogenic consequence. This mini-focus brings together a set of articles that explores key issues in analyzing, integrating and applying single-cell data with an emphasis on challenges for the field. In This Issue Top In this issue   ppvii - vii doi:10.1038/nbt.3732 Editorials Top Single-cell microbiology   p1077 doi:10.1038/nbt.3728 Microbiologists are poised to embrace heterogeneity through the use of single-cell technologies. Railroading at the FDA   p1078 doi:10.1038/nbt.3733 The US Food and Drug Administration approved a muscular-dystrophy drug against the scientific advice of its own staff and advisors. Despite leadership's attempts to downplay the controversy, doubts now surround standards for accelerated approval. News Top CAR-T cells forge ahead, Novartis reorganizes   pp1079 - 1081 Cormac Sheridan doi:10.1038/nbt1116-1079 Opdivo's lung cancer aspirations dashed   p1082 doi:10.1038/nbt1116-1082a Hope for anti-amyloid antibodies surges, yet again   pp1082 - 1083 Chris Morrison doi:10.1038/nbt1116-1082b Amgen's migraine antibody advances   p1084 doi:10.1038/nbt1116-1084a Zika pipeline progresses   pp1084 - 1086 Laura DeFrancesco doi:10.1038/nbt1116-1084b Mutanome draws Genentech deal   p1085 doi:10.1038/nbt1116-1085 3D cancer cells library   p1086 doi:10.1038/nbt1116-1086 Sanofi and Google in type 2 diabetes smartphone tie-up   pp1087 - 1088 Eric Smalley doi:10.1038/nbt1116-1087 Correction Top Corrections   p1088 doi:10.1038/nbt1116-1088 News Top Data Page Drug pipeline: 3Q16   p1089 Laura DeFrancesco doi:10.1038/nbt.3731 News Feature The cancer bloodhounds   pp1090 - 1094 Sarah Webb doi:10.1038/nbt.3717 Detecting cancer by liquid biopsy is attracting a lot of money and hype. Can it deliver on its promise to transform oncology? Sarah Webb reports. Advertisement Nature Index 2016 Rising Stars:  The Nature Index 2016: Rising Stars supplement identifies the people and organizations that have the potential to ascend within the world of science. The rising stars are identified by harnessing the power of the Nature Index, which tracks high-quality research of over 8,000 global institutions.  Access the free supplement in full today!  Bioentrepreneur Top Building a business Venturing across the Atlantic   pp1095 - 1098 Rogier W Rooswinkel, Daan S W Berbers, Eric H J H M Claassen and Sander van Deventer doi:10.1038/nbt.3712 Opinion and Comment Top Correspondence SPLASH, a hashed identifier for mass spectra   pp1099 - 1101 Gert Wohlgemuth, Sajjan S Mehta, Ramon F Mejia, Steffen Neumann, Diego Pedrosa et al. doi:10.1038/nbt.3689 The discombobulation of de-identification   pp1102 - 1103 Mark Phillips and Bartha M Knoppers doi:10.1038/nbt.3696 Confounding factors in identification of disease-resilient individuals   pp1103 - 1104 David S Rosenblatt, David Watkins, Farrah Rajabi and Harvey L Levy doi:10.1038/nbt.3684 Regulation of synthetic biology under the Nagoya Protocol   pp1104 - 1105 Bruce S Manheim doi:10.1038/nbt.3716 Commentary Greener revolutions for all   pp1106 - 1110 Richard B Flavell doi:10.1038/nbt.3709 To ensure global food security for all, the adoption of crop improvement technologies is no longer just an option[mdash]it is an imperative. Features Top Single-cell analysis at the threshold   pp1111 - 1118 Xi Chen, J Christopher Love, Nicholas E Navin, Lior Pachter, Michael J T Stubbington et al. doi:10.1038/nbt.3721 A discussion of some of the challenges and promise of single-cell technology. Patents Myriad's impact on gene patents   pp1119 - 1123 Mateo Aboy, Kathleen Liddell, Johnathon Liddicoat and Cristina Crespo doi:10.1038/nbt.3719 Three years later, the landmark Myriad decision on gene patents has led to some striking and unforeseen implications. Recent patents in RNA-based therapies   p1124 doi:10.1038/nbt.3729 News and Views Top Microcantilevers track single-cell mass   pp1125 - 1126 Rashid Bashir doi:10.1038/nbt.3725 An array of microfluidic cantilevers measures the mass of single cells in a population over time and detects drug-induced changes in cell growth. See also: Research by Stevens et al. Defining cell type with chromatin profiling   pp1126 - 1128 Mikhail Spivakov and Peter Fraser doi:10.1038/nbt.3724 Enhancer cytometry outperforms gene expression profiling in identifying hematopoietic cell subtypes. High-yield chemical synthesis by reprogramming central metabolism   pp1128 - 1129 Vivian Y Yu and Michelle C Y Chang doi:10.1038/nbt.3723 Altering the hardwired stoichiometry of central metabolism in yeast enables efficient synthesis of the isoprenoid [beta]-farnesene. Research Highlights   p1129 doi:10.1038/nbt.3720 Biotechnology JOBS of the week Postdoc University of California - Davis President & CEO Centre for Drug Research and Development (CDRD) Scientist II, Computational Chemistry Celgene Corporation Professor (Open Rank) - Department of Bioengineering University of Illinois Vector Biologist Texas Tech University More Science jobs from Biotechnology EVENT World Biotechnology Conference September 18, 2017 London, UK More science events from Research Top Analysis A multicenter study benchmarks software tools for label-free proteome quantification   pp1130 - 1136 Pedro Navarro, Jorg Kuharev, Ludovic C Gillet, Oliver M Bernhardt, Brendan MacLean et al. doi:10.1038/nbt.3685 LFQbench, a software tool to assess the quality of label-free quantitative proteomics analyses, enables developers to benchmark and improve analytic methods. Perspective Challenges in long-term imaging and quantification of single-cell dynamics   pp1137 - 1144 Stavroula Skylaki, Oliver Hilsenbeck and Timm Schroeder doi:10.1038/nbt.3713 The experimental and computational tools that enable continuous imaging of single cells for days and weeks have advanced rapidly in recent years, and solutions to current limitations are on the horizon. Review Revealing the vectors of cellular identity with single-cell genomics   pp1145 - 1160 Allon Wagner, Aviv Regev and Nir Yosef doi:10.1038/nbt.3711 Computational methods for analyzing single-cell data are uncovering new ways of defining cells. Articles Drug sensitivity of single cancer cells is predicted by changes in mass accumulation rate   pp1161 - 1167 Mark M Stevens, Cecile L Maire, Nigel Chou, Mark A Murakami, David S Knoff et al. doi:10.1038/nbt.3697 The efficacy of cancer drugs is profiled by measuring changes in the mass of single tumor cells. See also: News and Views by Bashir Differentiation of human embryonic stem cells to HOXA + hemogenic vasculature that resembles the aorta-gonad-mesonephros   pp1168 - 1179 Elizabeth S Ng, Lisa Azzola, Freya F Bruveris, Vincenzo Calvanese, Belinda Phipson et al. doi:10.1038/nbt.3702 Human embryonic stem cells are differentiated to cells similar to the embryonic aorta-gonad-mesonephros, which gives rise to hematopoietic stem cells. Genome-scale high-resolution mapping of activating and repressive nucleotides in regulatory regions   pp1180 - 1190 Jason Ernst, Alexandre Melnikov, Xiaolan Zhang, Li Wang, Peter Rogov et al. doi:10.1038/nbt.3678 The activating and repressing nucleotides of regulatory regions are identified at a genome-wide scale using tiling reporter assays. Resources Mitochondrial protein functions elucidated by multi-omic mass spectrometry profiling   pp1191 - 1197 Jonathan A Stefely, Nicholas W Kwiecien, Elyse C Freiberger, Alicia L Richards, Adam Jochem et al. doi:10.1038/nbt.3683 Proteomics, lipidomics and metabolomics of single gene deletion yeast strains sheds light on mitochondrial protein biology. A proteomic atlas of the legume Medicago truncatula and its nitrogen-fixing endosymbiont Sinorhizobium meliloti    pp1198 - 1205 Harald Marx, Catherine E Minogue, Dhileepkumar Jayaraman, Alicia L Richards, Nicholas W Kwiecien et al. doi:10.1038/nbt.3681 A proteomic atlas of a model legume and its rhizobial symbiont provides a resource for understanding symbiotic nitrogen fixation. Erratum Erratum: Defining digital medicine   p1206 Eric Elenko, Lindsay Underwood and Daphne Zohar doi:10.1038/nbt1116-1206 Careers and Recruitment Top Third-quarter biotech job picture   p1207 Michael Francisco doi:10.1038/nbt.3726 People People   p1208 doi:10.1038/nbt.3730 Advertisement nature.com webcasts Join us for our upcoming webcast with live Q&A: ADVANCES IN PRECISION MEDICINE AND GENOMIC SEQUENCING Presented by BioPharma Dealmakers Date: Tuesday, November 29 2016 Time: 8AM PST, 11AM EST, 4PM GMT, 5PM CET  REGISTER FOR FREE Sponsored by: Aperiomics Inc. | Bluebee | iNanoBio | Becton Dickinson (BD)     Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. 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