Nature Cell Biology contents: November 2016 Volume 18 Number 11, pp 1111 - 1260

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TABLE OF CONTENTS November 2016 Volume 18, Issue 11 Perspective News and Views Articles Letters Corrigendum Erratum Advertisement   OPENING NEW DOORS TO A WHOLE NEW VIEW OF BIOLOGY Go beyond snapshots and analyze global protein dynamics more deeply by harnessing the true power of accurate TMT-based quantitative proteomics. A new web resource highlighting critical advances across the entire workflow is now available! Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement A new cell line has to be established in the lab. What steps are necessary to ensure you are working with the right cells? How to treat new cell lines and what differences apply when handling cell lines already established? Does that sound familiar to you? Get the answers to these and other questions related to cell cultivation from the Eppendorf cell handling experts. Click here   Advertisement Nature Insight: Intestinal Microbiota in Health and Disease This Insight explores human development biology and how intestinal microbiota is associated closely with health and disease. Click here to access the free Nature Insight available for 6 months Produced with support from Yakult Honsha Co., Ltd.     Advertisement nature.com webcasts Springer Nature presents a custom webcast on Advancements in single-cell RNA-seq: differential expression analysis and immune profiling November 3, 2016; 9AM PDT, 12PM EDT, 4PM GMT, 5PM CET Learn about the latest advancements in single-cell RNA-seq and its application in whole transcriptome analysis and immune profiling.  Register for FREE Sponsored by  Takara Bio USA, Inc.     Perspective Top Progress towards generation of human haematopoietic stem cells   pp1111 - 1117 Lara Wahlster and George Q. Daley doi:10.1038/ncb3419 De novo generation of haematopoietic stem cells from different human pluripotent stem cell sources remains a high priority for haematology and regenerative medicine. At present, efficient derivation of functional haematopoietic stem cells with the capability for definitive in vivo engraftment and multi-lineage potential remains challenging. Here, we discuss recent progress and strategies to overcome obstacles that have thwarted past efforts. In addition, we review promising advances in the generation of mature blood lineages and the potential of induced pluripotent stem cells. News and Views Top On keeping the right ER size   pp1118 - 1119 Sebastian Schuck doi:10.1038/ncb3430 The endoplasmic reticulum (ER) is the largest membrane-bound organelle in cells, and its size needs to be carefully controlled. Downsizing the ER by autophagy is now shown to involve Sec62, a protein that also helps to build up the organelle. This link suggests a molecular switch for ER size control. See also: Article by Fumagalli et al. Activating ATR, the devil's in the dETAA1l   pp1120 - 1122 Wojciech Niedzwiedz doi:10.1038/ncb3431 Two studies now show that Ewing's tumour-associated antigen 1 (ETAA1) is recruited to sites of DNA replication stress through its interaction with replication protein A, where it stimulates the ATR kinase to promote efficient genome duplication. These findings provide exciting insight into the already very complex regulatory mechanism of the ATR activation cascade. See also: Article by Haahr et al. | Article by Bass et al. Targeting mutant p53 through the mevalonate pathway   pp1122 - 1124 William Freed-Pastor and Carol Prives doi:10.1038/ncb3435 It is well established that mutant forms of the p53 tumour suppressor acquire pro-oncogenic activities. Inhibition of the mevalonate pathway is now shown to promote degradation of select oncogenic mutant p53 proteins, indicating that destabilization of mutant p53 could be a promising therapeutic strategy. See also: Article by Parrales et al. A new bookmark of the mitotic genome in embryonic stem cells   pp1124 - 1125 Chris C.-S. Hsiung and Gerd A. Blobel doi:10.1038/ncb3432 Embryonic stem cells maintain pluripotency through countless mitoses. A recent report shows that the transcription factor Esrrb remains bound to chromatin during mitosis, including at regulatory regions that support pluripotency. Mitotic chromatin occupancy by Esrrb might stabilize the defining transcriptional programmes of embryonic stem cells through cell division. See also: Article by Festuccia et al. Nature Cell Biology JOBS of the week Post-doctoral position: RNA Epitranscriptome Bioinformatics and Stem Cell Biology Lund University Faculty Researcher in Stem Cell Biology at Stanford University Stanford University Post doc at Lund University Diabetes Centre Lund University Post-doc on T regulatory cells Inserm Retinal Cell Biologist UW-Madison, SMPH Department of Ophthalmology & Visual Sciences More Science jobs from Nature Cell Biology EVENT More science events from Advertisement The Naturejobs Career Expo is returning to Düsseldorf.   Friday 18, November 2016 This free career fair offers talented scientists an excellent opportunity to meet a diverse selection of national and international employers from academic institutions and scientific industries, such as pharmaceutical organisations, digital technology companies, science publishing and much more. Register free today!   Articles Top Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a   pp1127 - 1138 Alexandros Strikoudis, Charalampos Lazaris, Thomas Trimarchi, Antonio L. Galvao Neto, Yan Yang et al. doi:10.1038/ncb3424 Strikoudis et al. show that Phf5a is necessary for ESC self-renewal, efficient iPSC reprogramming and contributes to muscle specification by stabilizing Paf1C and controlling RNA polymerase II elongation. Mitotic binding of Esrrb marks key regulatory regions of the pluripotency network   pp1139 - 1148 Nicola Festuccia, Agnès Dubois, Sandrine Vandormael-Pournin, Elena Gallego Tejeda, Adrien Mouren et al. doi:10.1038/ncb3418 Festuccia et al. show that the pluripotency regulator Esrrb is retained on mitotic chromosomes, both in embryonic stem cells and during early embryogenesis, and epigenetically marks key regulatory regions during mitosis. See also: News and Views by Hsiung & Blobel The actin cable is dispensable in directing dorsal closure dynamics but neutralizes mechanical stress to prevent scarring in the Drosophila embryo   pp1149 - 1160 Antoine Ducuing and Stéphane Vincent doi:10.1038/ncb3421 Two studies by Pasakarnis et al. and Ducuing and Vincent show that the actin cable does not drive dorsal closure, but facilitates closure of the epidermis by providing zipping integrity and homogenizing mechanical tension along the leading edge. Amnioserosa cell constriction but not epidermal actin cable tension autonomously drives dorsal closure   pp1161 - 1172 Laurynas Pasakarnis, Erich Frei, Emmanuel Caussinus, Markus Affolter and Damian Brunner doi:10.1038/ncb3420 Two studies by Pasakarnis et al. and Ducuing and Vincent show that the actin cable does not drive dorsal closure, but facilitates closure of the epidermis by providing zipping integrity and homogenizing mechanical tension along the leading edge. Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery   pp1173 - 1184 Fiorenza Fumagalli, Julia Noack, Timothy J. Bergmann, Eduardo Cebollero Presmanes, Giorgia Brambilla Pisoni et al. doi:10.1038/ncb3423 Fumagalli et al. show that Sec62 delivers ER components to the autolysosome for clearance by acting as a receptor for autophagy protein LC3-II. This identifies Sec62 as a critical factor for selective ER turnover. See also: News and Views by Schuck ETAA1 acts at stalled replication forks to maintain genome integrity   pp1185 - 1195 Thomas E. Bass, Jessica W. Luzwick, Gina Kavanaugh, Clinton Carroll, Huzefa Dungrawala et al. doi:10.1038/ncb3415 Bass et al. and Haahr et al. identify ETAA1 as a critical replication stress response factor that interacts with DNA damage response proteins and activates ATR to maintain genomic stability. See also: Article by Haahr et al. | News and Views by Niedzwiedz Activation of the ATR kinase by the RPA-binding protein ETAA1   pp1196 - 1207 Peter Haahr, Saskia Hoffmann, Maxim A. X. Tollenaere, Teresa Ho, Luis Ignacio Toledo et al. doi:10.1038/ncb3422 Bass et al. and Haahr et al. now identify ETAA1 as a critical replication stress response factor that interacts with DNA damage response proteins and activates ATR to maintain genomic stability. See also: Article by Bass et al. | News and Views by Niedzwiedz Meiotic DNA break formation requires the unsynapsed chromosome axis-binding protein IHO1 (CCDC36) in mice   pp1208 - 1220 Marcello Stanzione, Marek Baumann, Frantzeskos Papanikos, Ihsan Dereli, Julian Lange et al. doi:10.1038/ncb3417 In meiosis, double-strand breaks (DSBs) are induced to initiate chromosome pairing and synapsis. Stanzione et al. identify IHO1 as a protein recruited by HORMAD1 to unsynapsed chromosome axes and required for DSB formation. Snail1-dependent p53 repression regulates expansion and activity of tumour-initiating cells in breast cancer   pp1221 - 1232 Ting Ni, Xiao-Yan Li, Na Lu, Teng An, Zhi-Ping Liu et al. doi:10.1038/ncb3425 Ni et al. report that Snail1 promotes mammary tumour initiation and maintenance independently of its role in EMT. They show that Snail1 forms a complex with HDAC1 and p53 that results in p53 inactivation and degradation, permitting tumour formation. DNAJA1 controls the fate of misfolded mutant p53 through the mevalonate pathway   pp1233 - 1243 Alejandro Parrales, Atul Ranjan, Swathi V. Iyer, Subhash Padhye, Scott J. Weir et al. doi:10.1038/ncb3427 Iwakuma and colleagues report that statins, through their action on the mevalonate pathway, lead to the ubiquitin-mediated degradation of misfolded mutant p53 by impairing its interaction with the Hsp40 family member, DNAJA1. See also: News and Views by Freed-Pastor & Prives Advertisement Open for Submissions  npj Precision Oncology is a new open access, online-only, peer-reviewed journal committed to publishing cutting-edge scientific research in all aspects of precision oncology from basic science to translational applications, to clinical medicine. The journal is part of the Nature Partner Journals series and published in partnership with The Hormel Institute, University of Minnesota.  Explore the benefits of submitting your manuscript >>   Letters Top An interaction between Scribble and the NADPH oxidase complex controls M1 macrophage polarization and function   pp1244 - 1252 Weiyue Zheng, Masataka Umitsu, Ishaan Jagan, Charles W. Tran, Noboru Ishiyama et al. doi:10.1038/ncb3413 Muthuswamy et al. report that in macrophages SCRIB interacts with the NADPH oxidase complex to promote the production of reactive oxygen species needed to kill bacteria. Conversely, loss of SCRIB promotes M1 macrophage polarization and inflammation. Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes   pp1253 - 1259 Alexander Leithner, Alexander Eichner, Jan Muller, Anne Reversat, Markus Brown et al. doi:10.1038/ncb3426 By modulating the presence of lamellipodia and filopodia, Sixt and colleagues determine that migrating dendritic cells rely on these protrusions for directed migration in complex environments, whereas locomotion per se is not driven by lamellipodia. Corrigendum Top Corrigendum: The RSPO-LGR4/5-ZNRF3/RNF43 module controls liver zonation and size   p1260 Lara Planas-Paz, Vanessa Orsini, Luke Boulter, Diego Calabrese, Monika Pikiolek et al. doi:10.1038/ncb3428 Erratum Top Erratum: Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow   p1260 Rachelle W. Johnson, Elizabeth C. Finger, Monica M. Olcina, Marta Vilalta, Todd Aguilera et al. doi:10.1038/ncb3433 Top Advertisement Nature Index 2016 Rising Stars:  The Nature Index 2016: Rising Stars supplement identifies the people and organizations that have the potential to ascend within the world of science. The rising stars are identified by harnessing the power of the Nature Index, which tracks high-quality research of over 8,000 global institutions.  Access the free supplement in full today!   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. 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