The EMBO Journal Table of Contents for 1 June 2016; Vol. 35, No. 11

1 June 2016 | Volume 35, Number 11 Submit Table of Contents Commentary News & Views Articles Volume 35, Number 11 Commentary Considerations for a European animal welfare standard to evaluate adverse phenotypes in teleost fish The EU Directive on the use animals in research requires scientists to assess and document pain, distress or lasting harm of genetically modified animals. This article proposes a detailed protocol and guidelines for assessing adverse phenotypes in teleost fish, an important model organism for biomedical research. Bettina Bert, Justyna Chmielewska, Sven Bergmann, Maximilian Busch, Wolfgang Driever, Karin Finger‐Baier, Johanna Hößler, Almut Köhler, Nora Leich, Thomas Misgeld, Torsten Nöldner, Annegret Reiher, Manfred Schartl, Anja Seebach‐Sproedt, Thomas Thumberger, Gilbert Schönfelder, and Barbara Grune Published online 22.04.2016 News & Views A tale of two α‐tubulin tails Microtubule tyrosination at the C‐terminus of α‐tubulin allows for robust initiation of mammalian dynein‐dynactin processivity, but tyrosination is dispensable once dynein is motile. Victoria J Allan Published online 19.04.2016 Scanning for a unified model for translational repression by microRNAs In light of the continuing debate on both the extent and mechanism of translational control by miRNAs, a new study aims to settle this question by directly comparing conflicting models for miRNA‐dependent repression of translation initiation. Tamiko Nishimura and Marc R Fabian Published online 19.04.2016 Articles Rac1‐Rab11‐FIP3 regulatory hub coordinates vesicle traffic with actin remodeling and T‐cell activation Rac1‐mediated actin cytoskeleton rearrangements required for normal T‐cell morphology and activation depend on association of Rac1 with Rab11‐FIP3‐positive recycling endosomes. Jérôme Bouchet, Iratxe del Río‐Iñiguez, Rémi Lasserre, Sonia Agüera‐Gonzalez, Céline Cuche, Anne Danckaert, Mary W McCaffrey, Vincenzo Di Bartolo, and Andrés Alcover Published online 06.05.2016 Tyrosination of α‐tubulin controls the initiation of processive dynein–dynactin motility Post‐translational modifications of tubulin can affect motor protein behavior on microtubules. This study reveals that microtubule tyrosination allows for robust initiation of mammalian dynein–dynactin processivity, but that tyrosination is dispensable once dynein is motile. Richard J McKenney, Walter Huynh, Ronald D Vale, and Minhajuddin Sirajuddin Published online 11.03.2016 miRISC and the CCR4–NOT complex silence mRNA targets independently of 43S ribosomal scanning Using a side‐by‐side comparison of fly and human cells, this work addresses conflicting models for miRNA‐mediated translational repression and concludes that silencing occurs in the absence of mRNA 5′‐UTR scanning. Duygu Kuzuoğlu‐Öztürk, Dipankar Bhandari, Eric Huntzinger, Maria Fauser, Sigrun Helms, and Elisa Izaurralde Published online 23.03.2016 Functional role of TRIM E3 ligase oligomerization and regulation of catalytic activity Structural and biochemical characterization of oligomeric ubiquitin ligases TRIM25 and TRIM32 shows diverse mechanisms mediating RING dimerization, which can occur in an intra‐ or intermolecular manner. Marios G Koliopoulos, Diego Esposito, Evangelos Christodoulou, Ian A Taylor, and Katrin Rittinger Published online 06.05.2016 The retinal pigment epithelium as a gateway for monocyte trafficking into the eye The retinal pigment epithelium acts as the primary entry point for infiltrating monocytes during retinal injury to positively contribute to the restoration of immunological balance in the retinal milieu. Inbal Benhar, Kitty Reemst, Vyacheslav Kalchenko, and Michal Schwartz Published online 22.04.2016 Presynaptic inhibition upon CB1 or mGlu2/3 receptor activation requires ERK/MAPK phosphorylation of Munc18‐1 ERK‐dependent Munc18‐1 phosphorylation provides an important negative feedback loop in glutamatergic hippocampal neurons to regulate synaptic strength. Sabine K Schmitz, Cillian King, Christian Kortleven, Vincent Huson, Tim Kroon, Josta T Kevenaar, Desiree Schut, Ingrid Saarloos, Joost P Hoetjes, Heidi de Wit, Oliver Stiedl, Sabine Spijker, Ka Wan Li, Huibert D Mansvelder, August B Smit, Lennart Niels Cornelisse, Matthijs Verhage, and Ruud F Toonen Published online 07.04.2016   Unsubscribe from or edit your subscription for this service. Or by mail: Customer Service * 425 Broadway St * Redwood City, CA 94063 * U.S.A. Copyright © 2016 by the European Molecular Biology Organisation.