Nature Chemical Biology Contents: June 2016, Volume 12 No 6 pp 381 - 466

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Advertisement Free naturejobs webcast From bench to business: Top tips from experts and entrepreneurs on how to take your research all the way from idea to product. You’ll also learn how to find the right funding and how to create the perfect pitch to get your research off the ground. Wednesday May 25th, 2016 at 8AM PST, 11AM EST, 4PM BST & 5PM CEST Register for the webcast and the live Q&A session Produced with support from: Novo Nordisk TABLE OF CONTENTS June 2016 Volume 12, Issue 6 Editorial Commentary Research Highlights News and Views Brief Communications Articles Erratum Advertisement Solve our challenge and win $200,000.  Identify novel small molecules that bind glucose and improve diabetes treatments. Visit our challenge here. Subscribe   Facebook   RSS   Recommend to library   Twitter   Editorial Top Lighting the way   p381 doi:10.1038/nchembio.2101 The innovation and optimization of optogenetic tools are providing new insights into biological and cellular processes with unparalleled spatiotemporal resolution. Commentary Top Designing convergent chemistry curricula   pp382 - 386 Louise K Charkoudian, Nicole S Sampson, Krishna Kumar and Joshua A Kritzer doi:10.1038/nchembio.2090 Scientific convergence is a common theme of modern research, but undergraduate chemistry is commonly taught as an isolated discipline. Here we discuss curricular updates at three different institutions that are independently seeking to increase convergence in introductory chemistry courses. Research Highlights Top Genomics: A Tribe for RNA | Gene expression: Means to an end | Protein evolution: Polymerase's new language | Host-microbe interactions: Inflammation sop-up News and Views Top Proteostasis: Chaperoning for hearing loss   pp388 - 389 Gergely L Lukacs doi:10.1038/nchembio.2091 A small molecule has been identified that can mitigate the progression of an inherited form of deafness in transgenic mice by stabilizing mutant clarin-1, an essential component of a multimolecular complex of the mechanotransduction apparatus of hair cells in the inner ear. See also: Article by Alagramam et al. Cancer metabolism: Addicted to serine   pp389 - 390 Christian Frezza doi:10.1038/nchembio.2086 Phosphoglycerate dehydrogenase (PHGDH) is an enzyme of serine biosynthesis overexpressed in various types of cancer. A new series of PHGDH inhibitors selectively block proliferation of PHGDH-dependent cancer cells and reveal an unexpected role of serine biosynthesis in coordinating one-carbon metabolism. See also: Article by Pacold et al. Metalloproteins: Mapping elusive electron density   pp391 - 392 Louis Noodleman doi:10.1038/nchembio.2088 Iron-sulfur metalloproteins are critical for electron transfer in bacterial metabolism, but most crystal structures are insufficient for their in-depth study. Now, acquisition of an iron-sulfur protein structure at ultra-high resolution enables detailed visualization of its electron distribution. Chemical Biology JOBS of the week Postdoctoral Position in Chemical Biology of Tubulin Modifications : Lausanne, Switzerland EPFL Swiss Federal Institute of Technology Lausanne Postdoctoral Associate Yale University PhD position in physiology Uppsala university, Department of Medical Cell Biology Uppsala University More Science jobs from Chemical Biology EVENT Chemical Biology Approaches to Assessing and Modulating Mitochondria 26 - 27 September 2016 Buckinghamshire, United Kingdom More science events from Brief Communications Top A synthetic biochemistry module for production of bio-based chemicals from glucose   pp393 - 395 Paul H Opgenorth, Tyler P Korman and James U Bowie doi:10.1038/nchembio.2062 A synthetic biochemistry approach optimizes a glucose breakdown pathway to produce acetyl-CoA as a building block for polyhydroxybutyrate bioplastic production in the test tube. The Taf14 YEATS domain is a reader of histone crotonylation   pp396 - 398 Forest H Andrews, Stephen A Shinsky, Erin K Shanle, Joseph B Bridgers, Anneliese Gest et al. doi:10.1038/nchembio.2065 Crotonylated lysine residues within histones are linked to transcriptional activation in a process involving histone mark 'reader' proteins. Crystallographic analysis of the YEATS domain of the Taf14 protein reveals a mode of crotonylated histone mark recognition via a π-sandwich motif. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications   pp399 - 401 Hayretin Yumerefendi, Andrew Michael Lerner, Seth Parker Zimmerman, Klaus Hahn, James E Bear et al. doi:10.1038/nchembio.2068 The attachment of a nuclear export sequence to the blue light-sensitive LOV2 domain mediates rapid and reversible protein export of the ubiquitin ligase Bre1 with light exposure, resulting in changes in histone ubiquitylation and methylation. Articles Top Raft-based interactions of gangliosides with a GPI-anchored receptor   pp402 - 410 Naoko Komura, Kenichi G N Suzuki, Hiromune Ando, Miku Konishi, Machi Koikeda et al. doi:10.1038/nchembio.2059 Monitoring new fluorescent ganglioside analogs at a single-molecule level suggests that gangliosides associate dynamically with GPI-anchored protein monomers, transient homodimer rafts, and clustered signaling rafts in a cholesterol-dependent manner. Chemical compounds Decoding Polo-like kinase 1 signaling along the kinetochore-centromere axis   pp411 - 418 Robert F Lera, Gregory K Potts, Aussie Suzuki, James M Johnson, Edward D Salmon et al. doi:10.1038/nchembio.2060 A chemical genetic approach to localize human Polo-like kinase 1 (Plk1) to distinct regions along the kinetochore-centromere axis combined with phosphoproteomic analysis reveals the presence of distinct Plk1 pools to mediate chromosomal segregation. Plant-like biosynthesis of isoquinoline alkaloids in Aspergillus fumigatus   pp419 - 424 Joshua A Baccile, Joseph E Spraker, Henry H Le, Eileen Brandenburger, Christian Gomez et al. doi:10.1038/nchembio.2061 Analysis of orphan nonribosomal peptide synthetase-like gene clusters from Aspergillus fumigatus identified a gene cluster responsible for the biosynthesis of fumisoquin isoquinoline alkaloids by amino acid condensation and subsequent tailoring steps reminiscent of plant biosynthetic pathways. Chemical compounds Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase   pp425 - 430 Amir Taslimi, Brian Zoltowski, Jose G Miranda, Gopal P Pathak, Robert M Hughes et al. doi:10.1038/nchembio.2063 Identification of minimal functional CRY2-CIB1 domains and mutations that increased CRY2 photocycle lifetimes combined with the development of an improved photoactivable Cre recombinase enables efficient gene editing. Optogenetic oligomerization of Rab GTPases regulates intracellular membrane trafficking   pp431 - 436 Mai Khanh Nguyen, Cha Yeon Kim, Jin Man Kim, Byung Ouk Park, Sangkyu Lee et al. doi:10.1038/nchembio.2064 An optogenetic approach using the CRY2-CIB1 heterodimerization system results in light-mediated cross-linking and aggregation of different Rab GTPases, disrupting membrane and endosomal trafficking in various cell types. Real-time monitoring of basal H2O2 levels with peroxiredoxin-based probes   pp437 - 443 Bruce Morgan, Koen Van Laer, Theresa N E Owusu, Daria Ezerina, Daniel Pastor-Flores et al. doi:10.1038/nchembio.2067 A fluorescent sensor combining a mutated form of the 2-Cys peroxiredoxin Tsa2 unable to undergo thioredoxin-mediated reduction with a redox-sensitive GFP protein allows real-time detection of baseline hydrogen peroxide levels in yeast cells. A small molecule mitigates hearing loss in a mouse model of Usher syndrome III   pp444 - 451 Kumar N Alagramam, Suhasini R Gopal, Ruishuang Geng, Daniel H-C Chen, Ina Nemet et al. doi:10.1038/nchembio.2069 A small molecule identified from high-throughput screening and optimization specifically stabilizes a disease-linked CLRN1 protein, delivering it to its site of action at the plasma membrane and preserving hearing in a new mouse model for Usher syndrome type III. Chemical compounds See also: News and Views by Lukacs A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate   pp452 - 458 Michael E Pacold, Kyle R Brimacombe, Sze Ham Chan, Jason M Rohde, Caroline A Lewis et al. doi:10.1038/nchembio.2070 A quantitative high-throughput screen identified an inhibitor of phosphoglycerate dehydrogenase (PHGDH), a key enzyme for serine synthesis. This inhibitor limits one-carbon unit availability for nucleotide synthesis. Chemical compounds See also: News and Views by Frezza Click-EM for imaging metabolically tagged nonprotein biomolecules   pp459 - 465 John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera et al. doi:10.1038/nchembio.2076 A new technique called click-EM uses singlet oxygen-generating fluorescent dyes and correlated light microscopy and EM to metabolically label and visualize nucleic acids and lipids at high resolution in cultured neurons and cells. Erratum Top Erratum: Chemoproteomic profiling of host and pathogen enzymes active in cholera   p466 Stavroula K Hatzios, Soren Abel, Julianne Martell, Troy Hubbard, Jumpei Sasabe et al. doi:10.1038/nchembio0616-466 Top Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. 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