Nature Biotechnology Contents: Volume 34 pp 115 - 212

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Advertisement A*STAR Research - Highlighting the best of research at the Agency for Science, Technology and Research (A*STAR), Singapore's premier research organization   Recent Highlights Medical imaging: Sharp images of moving tumors | Alzheimer's disease: Probing for oligomers | Diabetes: Insulin on demand  Download the A*STAR Research app now!   TABLE OF CONTENTS February 2016 Volume 34, Issue 2 Editorial News Correction Bioentrepreneur Opinion and Comment Features News and Views Computational Biology Research Careers and Recruitment Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement New! Nature Microbiology - First issue now published. The first issue of Nature Microbiology is now published and free to access online! This new journal covers all aspects of microorganisms, be it their evolution, physiology and cell biology; their interactions with each other, with a host or with an environment; or their societal significance. Ensure you stay up to date with future content by registering to receive the monthly e-alert.   Editorial Top Research not fit to print   p115 doi:10.1038/nbt.3488 Some biotech companies now eschew traditional publication in peer-reviewed journals. Does it matter? News Top FDA approves 'farmaceutical' drug from transgenic chickens   pp117 - 119 Cormac Sheridan doi:10.1038/nbt0216-117 Obama's cancer moonshot   p119 doi:10.1038/nbt0216-119 Biotechs target stagnant baldness market   pp120 - 121 Charles Schmidt doi:10.1038/nbt0216-120 CRISPR patents to go on trial   p121 doi:10.1038/nbt0216-121a Bayer joins CRISPR   p121 doi:10.1038/nbt0216-121b Proteasome play C4 debuts   p122 doi:10.1038/nbt0216-122a Direct Genomics revives Helicos sequencing system for China's hospitals   pp122 - 123 David Cyranoski doi:10.1038/nbt0216-122b New partner for Galapagos JAK drug   p123 doi:10.1038/nbt0216-123 Chimera research under the spotlight   pp124 - 125 Laura DeFrancesco doi:10.1038/nbt0216-124 Correction Top Corrections   p125 doi:10.1038/nbt0216-125a News Top Around the world in a month   p125 doi:10.1038/nbt0216-125b FDA approves three different multiple myeloma drugs in one month   p126 Mark Ratner doi:10.1038/nbt0216-126 Data Page 2015—another banner year for biotech   p127 Walter Yang doi:10.1038/nbt.3480 Drug pipeline Q4 2015   p128 Laura DeFrancesco doi:10.1038/nbt.3484 News Feature Fresh from the biotech pipeline—2015   pp129 - 132 Chris Morrison doi:10.1038/nbt.3472 Novel drug approvals in the United States surpassed even 2014's unprecedented totals. The broad trends are familiar but, as Chris Morrison reports, 2015 had its share of pioneering achievements. Advertisement Enhanced MALDI-TOF MS Performance and Flexibility  From a linear-only system to a unique QIT-TOF configuration to a high-resolution, high-energy TOF-TOF, Shimadzu's suite of MALDI mass spectrometers easily meets the needs of researchers with varying applications and budgets. Mine your data using our comprehensive software solutions. Learn more.   Bioentrepreneur Top Building a business Preparing for crises   pp133 - 136 Mark Kessel and Robert Masella doi:10.1038/nbt.3475 Opinion and Comment Top Correspondence Cas9-triggered chain ablation of cas9 as a gene drive brake   pp137 - 138 Bing Wu, Liqun Luo and Xiaojing J Gao doi:10.1038/nbt.3444 Precision and robustness of 2D-NMR for structure assessment of filgrastim biosimilars   pp139 - 141 Houman Ghasriani, Derek J Hodgson, Robert G Brinson, Ian McEwen, Lucinda F Buhse et al. doi:10.1038/nbt.3474 Evolving Japanese regulations on companion diagnostics   pp141 - 144 Sumimasa Nagai, Masaaki Urata, Hiroyuki Sato, Motoki Mikami, Wataru Kuga et al. doi:10.1038/nbt.3478 Features Top Patents Persistent confusion and controversy surrounding gene patents   pp145 - 147 Christi J Guerrini, Mary A Majumder and Amy L McGuire doi:10.1038/nbt.3470 The development of patent-specific educational resources and prompt resolution of patentability rules unsettled by recent US Supreme Court decisions are urgently needed in the genomics industry. Recent patents in computational biology   p148 doi:10.1038/nbt.3487 News and Views Top Can CRISPR-Cas9 gene drives curb malaria?   pp149 - 150 Luke Alphey doi:10.1038/nbt.3473 Gene drives in mosquitoes to reduce the spread of malaria move closer, though technical and regulatory hurdles remain. Mapping regulatory elements   pp151 - 152 Yuexin Zhou and Wensheng Wei doi:10.1038/nbt.3477 Regulatory genomic elements can now be studied in their native context using two CRISPR-based high-throughput approaches. See also: Research by Rajagopal et al. | Research by Korkmaz et al. Personalized nutrition through big data   pp152 - 154 Daniel McDonald, Gustavo Glusman and Nathan D Price doi:10.1038/nbt.3476 A systems model of glycemic response identifies individually tailored diets that keep blood sugar in check. Research Highlights   p154 doi:10.1038/nbt.3482 Biotechnology JOBS of the week Postdoctoral Fellows University of Turku More Science jobs from Biotechnology EVENT ECB2016 - 17th European Congress on Biotechnology July 3, 2016 Kraków, Poland More science events from Computational Biology Top Analysis Identifying recurrent mutations in cancer reveals widespread lineage diversity and mutational specificity   pp155 - 163 Matthew T Chang, Saurabh Asthana, Sizhi Paul Gao, Byron H Lee, Jocelyn S Chapman et al. doi:10.1038/nbt.3391 Detection of recurrently mutated nucleotides identifies novel cancer hotspots in an analysis of >11,000 human tumor samples. Research Top Brief Communications Antisense oligonucleotide-directed inhibition of nonsense-mediated mRNA decay   pp164 - 166 Tomoki T Nomakuchi, Frank Rigo, Isabel Aznarez and Adrian R Krainer doi:10.1038/nbt.3427 Antisense oligonucleotides target genetic disease via gene-specific inhibition of nonsense-mediated mRNA decay. Articles High-throughput mapping of regulatory DNA   pp167 - 174 Nisha Rajagopal, Sharanya Srinivasan, Kameron Kooshesh, Yuchun Guo, Matthew D Edwards et al. doi:10.1038/nbt.3468 Cis-regulatory genomic regions necessary for gene expression are revealed by a high-throughput CRISPR-Cas9-based assay. See also: News and Views by Zhou & Wei Integration of electrophysiological recordings with single-cell RNA-seq data identifies neuronal subtypes   pp175 - 183 Janos Fuzik, Amit Zeisel, Zoltan Mate, Daniela Calvigioni, Yuchio Yanagawa et al. doi:10.1038/nbt.3443 Patch-seq reveals new neuronal subtypes by combining electrophysiological and RNA-seq data on single neurons in situ. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9   pp184 - 191 John G Doench, Nicolo Fusi, Meagan Sullender, Mudra Hegde, Emma W Vaimberg et al. doi:10.1038/nbt.3437 Genome-wide sgRNA libraries based on rules for on-target activity improve results of Cas9-based screens and facilitate a further refinement of on- and off-target prediction algorithms. Letters Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9   pp192 - 198 Gozde Korkmaz, Rui Lopes, Alejandro P Ugalde, Ekaterina Nevedomskaya, Ruiqi Han et al. doi:10.1038/nbt.3450 CRISPR-Cas9 is used for both enrichment and dropout screens of functional enhancers in human cells. See also: News and Views by Zhou & Wei Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq   pp199 - 203 Cathryn R Cadwell, Athanasia Palasantza, Xiaolong Jiang, Philipp Berens, Qiaolin Deng et al. doi:10.1038/nbt.3445 Patch-seq reveals new neuronal subtypes by combining electrophysiological and RNA-seq data on single neurons in situ. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain   pp204 - 209 Benjamin E Deverman, Piers L Pravdo, Bryan P Simpson, Sripriya Ravindra Kumar, Ken Y Chan et al. doi:10.1038/nbt.3440 AAV vectors that efficiently transduce the mouse brain after intravenous injection are generated with a CRE-dependent selection system. Errata Erratum: Analysis of intronic and exonic reads in RNA-seq data characterizes transcriptional and post-transcriptional regulation   p210 Dimos Gaidatzis, Lukas Burger, Maria Florescu and Michael B Stadler doi:10.1038/nbt0216-210a Erratum: Share and share alike   p210 Samuel K Sia and Matthew P Owens doi:10.1038/nbt0216-210b Corrigendum Corrigendum: Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining   p210 Takeshi Maruyama, Stephanie K Dougan, Matthias C Truttmann, Angelina M Bilate, Jessica R Ingram et al. doi:10.1038/nbt0216-210c Careers and Recruitment Top Fourth-quarter biotech job picture   p211 Michael Francisco doi:10.1038/nbt.3479 People People   p212 doi:10.1038/nbt.3486 Advertisement Nature Conference on Tissue Engineering and Regenerative Medicine  Presented by: State Key Laboratory of Ophthalmology | Zhongshan Ophthalmic Center | Sun Yat-sen University Nature Materials | Nature Reviews Materials April 7-9, 2016 | Ocean Hotel, Guangzhou, China  Register now!   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. 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