Nature Methods Application Notes e-UPDATE: 12 January 2016

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12 January 2016  Application Notes bring you the latest information about innovative tools and technologies and their applications in the lab. We hope that you will find this service useful and informative and encourage you to sign up for future Updates to ensure that you never miss one!

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FEATURED APPLICATION NOTE The Airyscan detector from ZEISS: confocal imaging with improved signal-to-noise ratio and super-resolution www.zeiss.com > With Airyscan, ZEISS introduced a new detector concept for confocal laser-scanning microscopy (LSM). Whereas traditional LSM designs use a combination of pinhole and single-point detectors, Airyscan is a 32-channel gallium arsenide phosphide photomultiplier tube (GaAsP-PMT) area detector that collects a pinhole-plane image at every scan position. Each detector element functions as a single, very small pinhole. Knowledge about the beam path and the spatial distribution of each detector channel enables very light-efficient imaging with improved resolution and signal-to-noise ratio. PDF

		Nikon's large-format multiphoton system for intravital imaging www.nikon.com > Ultrafast resonant scanners; high-sensitivity, low-noise GaAsP detectors; and industry-leading optics have made Nikon's A1R MP multiphoton confocal microscope a system of choice for deep tissue imaging. Recent collaborative efforts between Nikon and the Allen Institute for Brain Science resulted in the design of an open-architecture multiphoton system able to image large specimens. Stimulated by this collaboration, Nikon has developed a commercially available large-format multiphoton system that can be used for the most demanding intravital imaging applications. PDF

		DNA assembly and cloning in an overnight run with the BioXp™ 3200 system www.syntheticgenomics.com > The BioXp™ 3200 system is an automated personal genomic workstation that builds and clones DNA fragments in a process that is virtually hands-free. In an overnight run, the instrument generates cloned DNA from custom-designed oligonucleotide pools and reagents engineered from sequence information. Here we discuss highlights and advantages of the BioXp system and the two modules currently available, the assembly module and the assembly-and-cloning module. PDF

		NGS library preparation for balanced, comprehensive methylome coverage from low-input quantities www.swiftbiosci.com > Next-generation sequencing (NGS) of bisulfite-converted DNA to detect methylation status with per-base resolution is currently restricted by the requirement of large amounts of input material. Here we show how the Accel-NGS® Methyl-Seq DNA library kit, with its unique chemistry, enables the construction of high-complexity libraries from 1 ng of input. With this kit, scientists will no longer be bforced to sacrifice library quality when using low quantities of starting material. PDF

		Automated live cell imaging of cell migration across a microfluidic-controlled chemoattractant gradient www.millipore.com > The quantification of cell-migration behavior in response to gradients is critical for understanding cellular movement and development. The CellASIC® ONIX microfluidic platform offers a microfluidic gradient plate to enable precision-controlled chemoattractant diffusion across perfusion barriers, enabling a quantitatively defined diffusion gradient stable enough for long-term, live cell analysis. Using the system, we studied the effect of a serum gradient on metastatic breast cancer cell migration distance, velocity and degree of chemotaxis. PDF

		The Role of Multiplexed Assays in Vaccine Development www.luminexcorp.com > Vaccine development is a complex and lengthy process, involving multiple stages. The goal of the first stage is to understand the disease burden and pathogenicity of a disease, including all of the variants of the disease-causing organism, and identify the antigens. Next, the preclinical stage of development begins with antigen characterization and purification, in order to develop vaccine candidates. PDF

		Optimized lysis system: ideal for extraction of genomic DNA from different kind of FFPE tissue samples www.analytik-jena.com > Analytik Jena's blackPREP FFPE DNA Kit is optimized for DNA extraction from a variety of FFPE (formalin-fixed, paraffin-embedded) tissue sample, e.g. adipose tissue, pancreatic tissue or placenta as starting materials. PDF

 

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