Nature Cell Biology contents: December 2015 Volume 17 Number 12, pp 1513 - 1607

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Advertisement Nikon's N-SIM E is a new personal structured illumination super-resolution system offering double the spatial resolution of conventional optical microscopes (to approximately 115 nm). N-SIM E encompasses a streamlined design, supports all commonly used excitation wavelengths and imaging modes, and is an affordable choice for individual labs. To learn more, please visit www.nikoninstruments.com/n-sim-e TABLE OF CONTENTS December 2015 Volume 17, Issue 12 Editorials News and Views Articles Letter Resource Technical Reports Addendum Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement SEE MORE. CLEARLY. Quick, flexible and affordable, Olympus will help you see those critical fine structures in your samples. The FV-OSR allows you to look deeper into your samples – not your budget. Take a closer look. Improved detection Great dye and wavelength flexibility Peltier cooling technology Your Science Matters at OLYMPUS' BOOTH #813. ASCB: December 12-16, 2015. San Diego.   Advertisement nature.com webcasts Join us for our upcoming webcast with live Q&A:  Developing next-generation antibody technologies Presented by BioPharma Dealmakers Date: Wednesday, December 16, 2015 Time: 8AM PST, 11AM EST, 4PM GMT, 5PM CET REGISTER FOR FREE  Sponsored by: Synaffix and AbSci   Editorials Top The long road to reproducibility   pp1513 - 1514 doi:10.1038/ncb3283 In the mission to reduce irreproducibility, true change can only come about if all stakeholders — researchers, institutions, funders and journals — join together with common purpose. Farewell to our chief   p1514 doi:10.1038/ncb3283 News and Views Top Rethinking glutamine addiction   pp1515 - 1517 Abigail S. Krall and Heather R. Christofk doi:10.1038/ncb3278 Tumours reprogram their metabolism to maximize macromolecule biosynthesis for growth. However, which of the common tumour-associated metabolic activities are critical for proliferation remains unclear. Glutamate-derived glutamine is now shown to satisfy the glutamine needs of glioblastoma, indicating that glutamine anaplerosis is dispensable for growth. See also: Article by Tardito et al. A motor relay on ciliary tracks   pp1517 - 1519 Robert O'Hagan and Maureen M. Barr doi:10.1038/ncb3279 A powerful combination of two-colour imaging in vivo, Fourier-filtered kymography and simulations provides a high-resolution view of kinesin-2 transport dynamics in cilia. This study reveals heterotrimeric kinesin-II as an 'obstacle-course runner' and homodimeric OSM-3 (KIF17) as a 'long-distance runner', and elucidates the 'baton handoff' between these two kinesin-2 motors on the microtubule track. See also: Article by Prevo et al. Metabolic exit from naive pluripotency   pp1519 - 1521 Jun Wu and Juan Carlos Izpisua Belmonte doi:10.1038/ncb3269 Spatiotemporally distinct pluripotent states captured in vitro provide an accessible way of modelling early human development. An intricate interplay between the metabolome and histone modifications is now shown to drive the metabolic switch from human naive to primed pluripotency, one of the earliest steps of embryogenesis. See also: Article by Sperber et al. Cell Biology JOBS of the week Senior / Principal Scientist, in vitro Cancer Cell Biology F. Hoffmann-La Roche AG Associate or Full Professor - Biochemistry and Cell Biology Stony Brook University PhD position: Decoding gene regulation in cancer KU Leuven, The Center for Human Genetics PhD Positions Ludwig-Maximilians-Universität Munich (LMU), Graduate School Life Science Munich Post-Doctoral Fellow University of Massachusett Medical School More Science jobs from Cell Biology EVENT The 2nd Conference on Advances in Cell Biology (CACB 2016) 18.03.16 Los Angeles, USA More science events from Advertisement Molecular Biology of the Cell, Sixth Edition by Alberts et al. A sample chapter and detailed table of contents are available at www.garlandscience.com/mboc6. Visit us as at Booth #1216 at the American Society for Cell Biology meeting in San Diego for more cell biology titles - 30% off.   Articles Top The metabolome regulates the epigenetic landscape during naive-to-primed human embryonic stem cell transition   pp1523 - 1535 Henrik Sperber, Julie Mathieu, Yuliang Wang, Amy Ferreccio, Jennifer Hesson et al. doi:10.1038/ncb3264 By comparing the metabolomes, transcriptomes and epigenomes of human pluripotent stem cell lines, Sperber et al. show that interplay between the metabolome and histone modifications drives the metabolic switch from naive to primed pluripotency. See also: News and Views by Wu & Belmonte Functional differentiation of cooperating kinesin-2 motors orchestrates cargo import and transport in C. elegans cilia   pp1536 - 1545 Bram Prevo, Pierre Mangeol, Felix Oswald, Jonathan M. Scholey and Erwin J. G. Peterman doi:10.1038/ncb3263 Using in vivo quantitative single-molecule fluorescence microscopy of kinesin II and OSM-3 motor dynamics in C. elegans cilia, Peterman and colleagues show that kinesin II loads cargo at the base, whereas OSM-3 transports the cargo to the tip. See also: News and Views by O'Hagan & Barr IRE1α is an endogenous substrate of endoplasmic-reticulum-associated degradation   pp1546 - 1555 Shengyi Sun, Guojun Shi, Haibo Sha, Yewei Ji, Xuemei Han et al. doi:10.1038/ncb3266 Through a proteomics approach, Qi and colleagues and Long and colleagues identify the sensor of the unfolded protein response IRE1α as an endogenous substrate of the E3 ubiquitin ligase involved in ER-associated degradation, Hrd1. Glutamine synthetase activity fuels nucleotide biosynthesis and supports growth of glutamine-restricted glioblastoma   pp1556 - 1568 Saverio Tardito, Anaïs Oudin, Shafiq U. Ahmed, Fred Fack, Olivier Keunen et al. doi:10.1038/ncb3272 Gottlieb and colleagues demonstrate that glioblastoma cell proliferation under glutamine starvation conditions depends on the glutamine-synthetase-dependent conversion of glutamate to glutamine to fuel purine biosynthesis and cell growth. See also: News and Views by Krall & Christofk Advertisement Start Imaging Fluorescence Without a Darkroom  Fluorescence is at your fingertips with ZOE™, a benchtop cell imager you control by touch screen. Features brightfield illumination and three fluorescent channels. Step out of the darkroom, see the light!   Letter Top Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes   pp1569 - 1576 Chie Hosono, Ryo Matsuda, Boris Adryan and Christos Samakovlis doi:10.1038/ncb3267 Samakovlis and colleagues perform a genome-wide, tissue-specific RNAi screen in the Drosophila larval and adult airway systems and find that an initial transient anisotropic distribution of aPKC drives fibre orientation during tube formation. Resource Top Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly   pp1577 - 1587 Edward R. Horton, Adam Byron, Janet A. Askari, Daniel H. J. Ng, Angélique Millon-Frémillon et al. doi:10.1038/ncb3257 Humphries and colleagues analyse proteomic data of integrin adhesion complexes to derive a consensus integrin adhesome and characterize the temporal dynamics of adhesome component recruitment during adhesion complex assembly and disassembly. Technical Reports Top A high-throughput platform for real-time analysis of membrane fission reactions reveals dynamin function   pp1588 - 1596 Srishti Dar, Sukrut C. Kamerkar and Thomas J. Pucadyil doi:10.1038/ncb3254 Pucadyil and colleagues develop an in vitro technique to analyse the conformational dynamics of dynamin during membrane fission events in a real-time, high-throughput manner, using fluorescence microscopy. Extracellular rigidity sensing by talin isoform-specific mechanical linkages   pp1597 - 1606 Katharina Austen, Pia Ringer, Alexander Mehlich, Anna Chrostek-Grashoff, Carleen Kluger et al. doi:10.1038/ncb3268 Austen et al. generated talin biosensors to study integrin-based force transduction. They report that extracellular rigidity sensing requires talin's mechanical engagement and find talin isoform-dependent effects in integrin-mediated mechanosensing. Addendum Top Addendum: A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages   p1607 Haihui Lu, Karl R. Clauser, Wai Leong Tam, Julia Frose, Xin Ye et al. doi:10.1038/ncb3281 Top Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

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