Tuesday, August 11, 2015

Nature Methods Application Notes e-UPDATE: 11 August 2015

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11 August 2015 
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FEATURED APPLICATION NOTE
Co-transfection controls with reporter assays
www.activemotif.com/catalog/900/lightswitch-luciferase-assay-system >
In most cases you do not need a co-transfection control. This technical bulletin offers guidelines and experimental data that will help you evaluate the needs of your reporter assay experiment.
PDF


Oxygen transport across slippery and curved gas-liquid interfaces using phosphorescence lifetime imaging
www.lambertinstruments.com >
The transport phenomena at interfaces often determine or limit the overall performance of processes. Direct investigations of interfacial transport of momentum, mass and heat at the interfaces in micron scale are highly appreciable for further optimization of various micro and macroscale technologies.
PDF

Water-In-Oil Droplets: Position Control With Liquid Electrodes
www.fluigent.com >
Demonstration of Electrowell-generated on-chip liquid electrodes to replace embedded electrodes.
PDF

Thermal shift assay using SYPRO® Orange and qTOWER 2.2 to detect protein melting temperatures
www.analytik-jena.com >
The stability of proteins depends on ligand interactions, buffer conditions or changes in conformation, which is traditionally investigated by time-consuming Circular Dichroism (CD) Spectroscopy. In addition the thermal-shift assay is based on temperature-induced denaturation and can be monitored by using SYPRO® Orange. This fluorescence dye is a naturally quenched dye and interacts with the hydrophobic core of proteins which is exposed after denaturation.
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Zoom Plate™ Dot Blot
www.vitrozm.com >
Zoom Plate is a novel 96 well assay platform for fast and sensitive membrane-based assays such as dot blot and other sandwich assays. Based on Vitrozm's patent pending vertical flow technology' Zoom Plate significantly simplifies the washing and incubation process in membrane-based assays' allowing fast and sensitive analysis without lengthy incubation or vacuum/centrifuge-assisted filtration. Users only need to pipette samples and reagents sequentially into the Zoom Plate wells' and get results in about 1 hour. Compared to traditional dot blot or ELISA' Zoom Blot only needs 1-10 μl of antibody or sample for each blot' a significant saving for large scale screening tests. Compatible with automatic liquid handling' Zoom Plate enables sensitive fluorescent' luminescent and colorimetric assays with excellent signal-to-noise ratio.
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Imaging flow cytometry enhances particle detection sensitivity for extracellular vesicle analysis
www.millipore.com >
The study of extracellular vesicles (EVs) has evolved into a compelling field of intercellular communication and pathophysiology research. The submicron size of EVs and the complexity of the biofluids in which they are suspended confound their analysis by traditional light microscopy or flow cytometry. Observation and quantitation of EVs have been achieved with Amnis® imaging flow cytometers (IFCs) with reduced volumes and sample preparation times, suggesting an auspicious role for IFCs in the advancement of EV biology.
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GelApp: Mobile gel electrophoresis analyser
www.a-star.edu.sg >
GelApp is a free mobile application that automatically detects and quantifies gel electrophoresis bands for "on-the-go" gel documenta-tion. It facilitates quantitative biological research by providing more accurate band sizes.Through the novel implementation of Gabor filters, faint bands can be better detected from noisy backgrounds than conventional image analysis methods. Plotting the marker standard as a logarithmic (log) graph, the sample band sizes are calculated more accurately. Combined, these features help increase the productivity and accu-racy of gel electrophoresis analysis that every scientist can carry in their pockets.
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