Nature Biotechnology Contents: Volume 33 pp 781 - 884

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Advertisement Q2 Solutions can enhance your precision medicine efforts  by providing comprehensive genomic and biomarker discovery and development expertise, scientific acumen, data informatics and clinical trial operational excellence. Let our experience and insights help you avoid obstacles and optimize your path to developing new personalized medicines and companion diagnostics. TABLE OF CONTENTS August 2015 Volume 33, Issue 8 Editorial News Bioentrepreneur Opinion and Comment Features News and Views Computational Biology Research Careers and Recruitment Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement Noncoding RNAs in Endocrinology  Noncoding RNAs have important roles in the development and regulation of the endocrine system. This web collection on noncoding RNAs in endocrinology highlights current and future applications of noncoding RNAs as diagnostic and prognostic biomarkers and as therapeutic targets for personalized management of patients with a wide range of endocrine diseases. Access the collection online.     Editorial Top Old boy biotech   p781 doi:10.1038/nbt.3308 No matter where you look in biopharmaceutical boardrooms, gender bias is evident. Is it so difficult to actually acknowledge this pernicious bias and actively discourage all-male boards? News Top 'Financial toxicity' looms as cancer combinations proliferate   pp783 - 784 Chris Morrison doi:10.1038/nbt0815-783 New class PCSK9 blockers stride into cholesterol market   pp785 - 786 Cormac Sheridan doi:10.1038/nbt0815-785 Microbial cocktails join fecal transplants in IBD treatment trials   pp787 - 788 Mark Ratner doi:10.1038/nbt0815-787 Blockbuster expectations for hepatitis B therapeutic vaccine   p789 Suzanne Elvidge doi:10.1038/nbt0815-789 NCI-MATCH pairs tumor mutations with matching drugs   pp790 - 791 Vicki Brower doi:10.1038/nbt0815-790 Alnylam, Dicerna tussle over RNAi tech   p791 doi:10.1038/nbt0815-791a Court invalidates Sequenom's prenatal test patent   p791 doi:10.1038/nbt0815-791b PureTech floats away   p792 doi:10.1038/nbt0815-792a BGI goes head-to-head with Illumina   p792 doi:10.1038/nbt0815-792b Oxitec trials GM sterile moth to combat agricultural infestations   pp792 - 793 Emily Waltz doi:10.1038/nbt0815-792c Around the world in a month   p793 doi:10.1038/nbt0815-793 Data Page 2Q15—Running of the biotech bulls   p794 Walter Yang doi:10.1038/nbt.3311 Drug pipeline: 2Q15   p795 Laura DeFrancesco doi:10.1038/nbt.3312 News Feature To share is human   pp796 - 800 doi:10.1038/nbt.3309 Personal health data are beginning to be shared in the digital world—on mobile devices and the internet. Is the loss of privacy a price worth paying for the presumed benefits? Laura DeFrancesco investigates. Advertisement Simple, Rapid, Reproducible Collection of Plasma  Offering significant advantages for use with LC-MS/MS instruments, Shimadzu's Noviplex Cards allow for rapid collection of a volumetric sample of plasma (2.5 µL) from an unmeasured amount of whole blood (20 to 75 µL) within minutes. They can be used as a single step, solid-phase method for sample preparation and analyte extraction. Learn more. Bioentrepreneur Top Building a business In house expertise   pp801 - 804 Orin Herskowitz doi:10.1038/nbt.3302 First Rounders Podcast   p804 Rachel King doi:10.1038/nbt0815-804 Opinion and Comment Top Correspondence Flexible guide-RNA design for CRISPR applications using Protospacer Workbench   pp805 - 806 Cameron Ross MacPherson and Artur Scherf doi:10.1038/nbt.3291 Health ROI as a measure of misalignment of biomedical needs and resources   pp807 - 811 Lixia Yao, Ying Li, Soumitra Ghosh, James A Evans and Andrey Rzhetsky doi:10.1038/nbt.3276 Benchmarking the scientific output of the Innovative Medicines Initiative   pp811 - 812 Magda Gunn, Mark Lim, Didi Cross and Michel Goldman doi:10.1038/nbt.3305 What does the current biotech stock market value?   pp813 - 814 Laura McNamee and Fred Ledley doi:10.1038/nbt.3303 Commentary The smart and gentle microscope   pp815 - 818 Nico Scherf and Jan Huisken doi:10.1038/nbt.3310 New instruments are needed to realize the potential of quantitative and systematic imaging of living samples. But what would such a microscope look like? Features Top Patents The emergence of agbiogenerics   pp819 - 823 David J Jefferson, Gregory D Graff, Cecilia L Chi-Ham and Alan B Bennett doi:10.1038/nbt.3306 Although the first major agbiotech product patent has expired, regulatory requirements could continue to allot a significant degree of control to the original right holder. Recent patents and applications in optogenetics   p824 doi:10.1038/nbt.3324 News and Views Top Deep learning for regulatory genomics   pp825 - 826 Yongjin Park and Manolis Kellis doi:10.1038/nbt.3313 Computational modeling of DNA and RNA targets of regulatory proteins is improved by a deep-learning approach. See also: Computational Biology by Alipanahi et al. A time and a place for sugar in your ears   pp827 - 828 Jiahn-Chou Guan and Karen E Koch doi:10.1038/nbt.3315 Targeting trehalose metabolism improves maize yield under a range of water-deficit conditions. See also: Research by Nuccio et al. Research Highlights   p828 doi:10.1038/nbt.3307 New PAMs for Cas   p829 Markus Elsner doi:10.1038/nbt.3317 A flexible mesh to record the brain   p830 Irene Jarchum doi:10.1038/nbt.3316 Computational Biology Top Analysis Predicting the sequence specificities of DNA- and RNA-binding proteins by deep learning   pp831 - 838 Babak Alipanahi, Andrew Delong, Matthew T Weirauch and Brendan J Frey doi:10.1038/nbt.3300 The binding specificities of RNA- and DNA-binding proteins are determined from experimental data using a /`deep learning/' approach. See also: News and Views by Park & Kellis Research Top Brief Communications Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery   pp839 - 841 Liliana Wroblewska, Tasuku Kitada, Kei Endo, Velia Siciliano, Breanna Stillo et al. doi:10.1038/nbt.3301 Using RNA-binding proteins to build genetic circuits enables RNA-only synthetic biology. Engineered CHO cells for production of diverse, homogeneous glycoproteins   pp842 - 844 Zhang Yang, Shengjun Wang, Adnan Halim, Morten Alder Schulz, Morten Frodin et al. doi:10.1038/nbt.3280 A knockout study of all human glycosyltransferases encoded in CHO cells provides a guide to producing proteins with desired glycosylation patterns. Articles Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation   pp845 - 852 Fotios Sampaziotis, Miguel Cardoso de Brito, Pedro Madrigal, Alessandro Bertero, Kourosh Saeb-Parsy et al. doi:10.1038/nbt.3275 A protocol for generating biliary epithelial cells from human pluripotent stem cells facilitates disease modeling and drug screening. Directed differentiation of cholangiocytes from human pluripotent stem cells   pp853 - 861 Mina Ogawa, Shinichiro Ogawa, Christine E Bear, Saumel Ahmadi, Stephanie Chin et al. doi:10.1038/nbt.3294 A protocol for generating biliary epithelial cells from human pluripotent stem cells facilitates disease modeling and drug screening. Expression of trehalose-6-phosphate phosphatase in maize ears improves yield in well-watered and drought conditions   pp862 - 869 Michael L Nuccio, Jeff Wu, Ron Mowers, Hua-Ping Zhou, Moez Meghji et al. doi:10.1038/nbt.3277 Expression of a single trehalose transgene in maize improves yield in field trials in both well-watered and drought conditions. See also: News and Views by Guan & Koch Letters Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown   pp870 - 876 Anders Wittrup, Angela Ai, Xing Liu, Peter Hamar, Radiana Trifonova et al. doi:10.1038/nbt.3298 Live-cell imaging provides new insights in the cytosolic release mechanismsof lipid-formulated siRNAs. High-throughput sequencing of DNA G-quadruplex structures in the human genome   pp877 - 881 Vicki S Chambers, Giovanni Marsico, Jonathan M Boutell, Marco Di Antonio, Geoffrey P Smith et al. doi:10.1038/nbt.3295 G-quadruplex structures are detected across the human genome using a high-throughput sequencing-based approach. Corrigenda Corrigendum: Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells   p882 Weida Li, Claudia Cavelti-Weder, Yinying Zhang, Kendell Clement, Scott Donovan et al. doi:10.1038/nbt0815-882a Corrigendum: The ethics of publishing human germline research   p882 Arun Sharma and Christopher Thomas Scott doi:10.1038/nbt0815-882b Corrigendum: Suppression of vascular permeability and inflammation by targeting of the transcription factor c-Jun   p882 Roger G Fahmy, Alla Waldman, Guishui Zhang, Ainslie Mitchell, Nicodemus Tedla et al. doi:10.1038/nbt0815-882c Errata Erratum: A mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides   p882 Joel M Chick, Deepak Kolippakkam, David P Nusinow, Bo Zhai, Ramin Rad et al. doi:10.1038/nbt0815-882d Erratum: Public biotech 2013—the numbers   p882 Stacy Lawrence and Riku Lahteenmaki doi:10.1038/nbt0815-882e Careers and Recruitment Top Second-quarter biotech job picture   p883 Michael Francisco doi:10.1038/nbt.3314 People People   p884 doi:10.1038/nbt.3320 Advertisement Nature Genetics in association with the Wellcome Trust present: THE GENOMICS OF COMMON DISEASES 2015 September 2-5, 2015 | Cambridge, UK  REGISTER NOW!   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. 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