Nature Methods Application Notes e-UPDATE: 12 May 2015

If you are unable to see the message below, click here to view.

Search/browse Application Notes

12 May 2015  Application Notes bring you the latest information about innovative tools and technologies and their applications in the lab. We hope that you will find this service useful and informative and encourage you to sign up for future Updates to ensure that you never miss one!

Register to continue receiving application notes updates

FEATURED APPLICATION NOTE Screening and quantification of the tumor microenvironment with micro-ultrasound and photoacoustic imaging www.visualsonics.com > The family of Vevo® Imaging Systems from FUJIFILM VisualSonics, Inc. is leading to translational breakthroughs in the understanding of cancer progression and therapy. More specifically, high-frequency ultrasound and photoacoustic imaging of orthotopic and patient-derived xenograft (PDX) models of human cancer have empowered researchers in the past decade, with key translational findings on tumor growth and microenvironment and in response to new and existing cancer therapies. This Application Note focuses on the deep-tissue-imaging capabilities of micro-ultrasound and photoacoustic imaging that contributed to these translational findings.. PDF

		Lab-on-a-Chip Application: Creating Parabolic and Plug-Like Flow Profiles in a Microchannel www.fluigent.com > The manipulation of reagents and samples for multiple purposes including reactions and analysis for biotechnology, nanotechnology, and chemistry applications requires reproducibility and simplicity to be useful in practice. As microfluidic devices transition from a research project in and of themselves to a platform utilized by scientists investigating problems in a wide variety of fields, the ability to reproducibly control fluid and flow profiles for predictable results becomes as important as the device itself. The two most common types of fluid flow generation are hydrodynamic or pressure driven flow and electroosmotic flow. Both have advantages and disadvantages. The chief advantage of electroosmotic flow is the plug like flow profile that prevents dispersion in downstream analysis. However, generating a linear electric field in a small channel is fraught with challenges including preventing bubbles formed at the electrode or gas in the solution from interfering with the application of a uniform electric field. To solve this problem a combination of pressure driven flow and electroosmotic flow has been harnessed. PDF

		Imaging flow cytometry enhances particle detection sensitivity for extracellular vesicle analysis www.millipore.com > The study of extracellular vesicles (EVs) has evolved into a compelling field of intercellular communication and pathophysiology research. The submicron size of EVs and the complexity of the biofluids in which they are suspended confound their analysis by traditional light microscopy or flow cytometry. Observation and quantitation of EVs have been achieved with Amnis® imaging flow cytometers (IFCs) with reduced volumes and sample preparation times, suggesting an auspicious role for IFCs in the advancement of EV biology. PDF

		Comparison of real-time signals using white or clear microplates www.analytik-jena.com > Analytik Jena's product family includes the standard real-time thermal cyclers qTOWER 2.0 and 2.2. Featuring a striking, modern design, these systems allow quantitative PCR in an established 96 well format. PDF

		SafeSeqr - Fast and Robust Food Authenticity Testing using Next-Generation Sequencing (NGS) www.sciencevision.com.my/ > SAFESeqr provides a complete solution for food authenticity test with the development of SAFESeqr Kit for NGS library preparation and SAFESeqr Suite analysis software. SAFESeqr delivers robust, specific, and fast identification of up to 1842 eukaryotic species and 1161 eukaryotic genus. Combining SAFESeqr one-step PCR library preparation workflow and the Illumina MiSeq NGS platform, it is capable of detecting resolving complex and low level sample mixtures requiring only 2ng of input DNA. SAFESeqr complete solution with the highest-quality data generated can be easily visualized and reported using the SAFESeqr Suite analysis software. PDF

		GelApp: Mobile gel electrophoresis analyser www.a-star.edu.sg > GelApp is a free mobile application that automatically detects and quantifies gel electrophoresis bands for "on-the-go" gel documenta-tion. It facilitates quantitative biological research by providing more accurate band sizes.Through the novel implementation of Gabor filters, faint bands can be better detected from noisy backgrounds than conventional image analysis methods. Plotting the marker standard as a logarithmic (log) graph, the sample band sizes are calculated more accurately. Combined, these features help increase the productivity and accu-racy of gel electrophoresis analysis that every scientist can carry in their pockets. PDF

		Exploring Protein Phosphorylation with RayBio® Antibody Arrays and ELISAs www.raybiotech.com > Phosphorylation is arguably the most important regulatory mechanism in biological systems, directing both the activity and subcellular localization of many key signaling proteins. Phosphorylation is often detected by isotope labeling or by 2D gel and mass spectrometry analyses. Both methods are highly sensitive. However, because of the high equipment cost and labor required for mass spectrometry, and the special isotope handling and disposal required for radiolabeling, phospho-specific antibodies are generally the more preferred tool for detection of protein phosphorylation. ELISAs can be designed to rapidly and inexpensively detect phosphorylation-induced conformational changes in a specific protein, allowing many samples to be tested in parallel. Alternatively, phospho-specific antibodies can be multiplexed into an array format, allowing the simultaneous detection of multiple target phospho-proteins. Using these powerful tools, researchers can accomplish either high throughput detection of a specific activated protein in multiple samples, or detect phosphorylation signatures through parallel detection of many related phospho-proteins. PDF

 

Application Notes are supplied by commercial companies. The Nature Publishing Group does not endorse any of the products or services advertised here. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department For print subscription enquiries, please contact our subscription department For other enquiries, please contact our feedback department Nature Publishing Group | 75 Varick Street, 9th Floor | New York | NY 10013-1917 | USA Nature Publishing Group's worldwide offices: London - Paris - Munich - New Delhi - Tokyo - Melbourne San Diego - San Francisco - Washington - New York - Boston