Nature Biotechnology Contents: Volume 33 pp 429 - 566

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Advertisement NEW WHITE PAPER: The Advantage & Application of Genetically Humanized Mouse Models for Biomedical Research  This white paper explores the technologies used to generate genetically humanized mice and explains their applications, including: Efficacy & Safety Testing of Therapeutic Compounds & Biologics Drug Metabolism & Disposition The Immune System Novel In Vivo Therapeutic Approaches Download Your Copy Today TABLE OF CONTENTS May 2015 Volume 33, Issue 5 Editorial News Correction Bioentrepreneur Opinion and Comment Features News and Views Computational Biology Research Careers and Recruitment Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Editorial Top Next-generation genome editing   p429 doi:10.1038/nbt.3234 Nature Biotechnology supports recent calls for public engagement concerning the risks and benefits of genome editing in the human germline, particularly given our poor knowledge of what we should change in the human genome. News Top CRISPR germline editing reverberates through biotech industry   pp431 - 432 Cormac Sheridan doi:10.1038/nbt0515-431 Startups use short-read data to expand long-read sequencing market   pp433 - 435 Michael Eisenstein doi:10.1038/nbt0515-433 FDA approves 23andMe gene carrier test   p435 doi:10.1038/nbt0515-435a Court sides with Sandoz over Neupogen biosimilar   p435 doi:10.1038/nbt0515-435b Human cell assays for new medicines now open access   pp436 - 437 Asher Mullard doi:10.1038/nbt0515-436 Keytruda UK's first early-access drug   p437 doi:10.1038/nbt0515-437a Epigenome drug approved after setback   p437 doi:10.1038/nbt0515-437b Biogen's early Alzheimer's data raise hopes, some eyebrows   p438 Mark Ratner doi:10.1038/nbt0515-438 First-in-class HIV drug enters phase 3 trials   p439 Anna Azvolinsky doi:10.1038/nbt0515-439a Correction Top Corrections   p439 doi:10.1038/nbt0515-439b News Top Lilly pays $50 million for Korean BTK   p440 Allison Proffitt doi:10.1038/nbt0515-440a Around the world in a month   p440 doi:10.1038/nbt0515-440b Data Page Quality traits reach market   p441 Andrew Marshall doi:10.1038/nbt.3201 Biotech continues to soar in 1Q15   p442 Walter Yang doi:10.1038/nbt.3229 Bioentrepreneur Top Data page Research institute partnerships 2014   pp443 - 444 Brady Huggett doi:10.1038/nbt.3214 SciCafe Startups on the menu: Atreca   p444 doi:10.1038/nbt.3226 Opinion and Comment Top Correspondence MutMap accelerates breeding of a salt-tolerant rice cultivar   pp445 - 449 Hiroki Takagi, Muluneh Tamiru, Akira Abe, Kentaro Yoshida, Aiko Uemura et al. doi:10.1038/nbt.3188 Precision medicine and the FDA's draft guidance on laboratory-developed tests   pp449 - 451 Thomas J Hwang, Lisa Soleymani Lehmann and Aaron S Kesselheim doi:10.1038/nbt.3221 What's behind a 'genetically engineered' label?   pp451 - 453 Graham Brookes and Henry I Miller doi:10.1038/nbt.3225 Transparency in GM food labeling   p453 Peter Melchett doi:10.1038/nbt.3220 Reply to: Nature Biotechnology Responds to Melchett Correspondence   pp453 - 454 doi:10.1038/nbt.3224 Oversight of human inheritable genome modification   pp454 - 455 Rosario Isasi and Bartha M. Knoppers doi:10.1038/nbt.3231 Commentary Defining digital medicine   pp456 - 461 Eric Elenko, Lindsay Underwood and Daphne Zohar doi:10.1038/nbt.3222 Digital medicine is poised to transform biomedical research, clinical practice and the commercial sector. Here we introduce a monthly column from R&D/venture creation firm PureTech tracking digital medicine's emergence. The digital phenotype   pp462 - 463 Sachin H Jain, Brian W Powers, Jared B Hawkins and John S Brownstein doi:10.1038/nbt.3223 In the coming years, patient phenotypes captured to enhance health and wellness will extend to human interactions with digital technology. What Ebola tells us about outbreak diagnostic readiness   pp464 - 469 Mark D Perkins and Mark Kessel doi:10.1038/nbt.3215 Healthcare priorities all too often ignore the importance of diagnostics for disease control and case management. The Ebola epidemic illustrates the folly of this attitude when few therapeutic or prophylactic interventions are available. Features Top Biotech's wellspring—a survey of the health of the private sector in 2014   pp470 - 477 Brady Huggett doi:10.1038/nbt.3218 Private biotech never had it so good. Although the number of startups formed did not increase dramatically, the sector grew in size and received much more money than in 2013. CRISPR germline engineering[mdash]the community speaks   pp478 - 486 Katrine S Bosley, Michael Botchan, Annelien L Bredenoord, Dana Carroll, R Alta Charo et al. doi:10.1038/nbt.3227 Nature Biotechnology asks selected members of the international community to comment on the ethical issues raised by the prospect of CRISPR-Cas9 engineering of the human germline. Patents A solution to the controversy on plant variety protection in Africa   pp487 - 488 Bram De Jonge, Niels P Louwaars and Julian Kinderlerer doi:10.1038/nbt.3213 African countries can establish a plant variety protection system that supports commercial seed systems without negatively affecting smallholder farmers. Recent patent applications in genome editing   p489 doi:10.1038/nbt.3233 News and Views Top Putting cells in their place   pp490 - 491 Omid R Faridani and Rickard Sandberg doi:10.1038/nbt.3219 A combination of single-cell transcriptomics with in situ hybridization information enables single cells to be positioned within their tissue. See also: Computational Biology by Satija et al. | Computational Biology by Achim et al. Unraveling the fabric of polyploidy   pp491 - 493 Andrew H Paterson and Jonathan F Wendel doi:10.1038/nbt.3217 Draft genome sequences of tetraploid Upland cotton and other polyploids promise insights into genome evolution and cotton fiber biology. See also: Research by Li et al. | Research by Zhang et al. Islet implantation in a pocket   pp493 - 494 Jonathan S Bromberg doi:10.1038/nbt.3216 Exploiting the foreign body reaction creates a prevascularized space that accommodates and supports transplanted islets. See also: Research by Pepper et al. Research Highlights   p494 doi:10.1038/nbt.3228 Biotechnology JOBS of the week professor in the field of Molecular Biotechnology Graz University of Technology Professor in Industrial Biotechnology The University of Manchester Research positions (1 Posdoc & 1 PhD) in stream biofilm ecology Ecole Polytechnique Fédérale de Lausanne (EPFL) JBEI Postdoctoral Appointee - Digital Microfluidics Sandia National Laboratories Executive Director & Chief Executive Officer The American Society for Microbiology More Science jobs from Biotechnology EVENT 2nd Biotechnology Transfer & Commercialization Conference September 10, 2015 Philadelphia, USA More science events from Computational Biology Top Analysis Spatial reconstruction of single-cell gene expression data   pp495 - 502 Rahul Satija, Jeffrey A Farrell, David Gennert, Alexander F Schier and Aviv Regev doi:10.1038/nbt.3192 RNA-seq data from single cells are mapped to their location in complex tissues using gene expression atlases based on in situ hybridization. See also: News and Views by Faridani & Sandberg High-throughput spatial mapping of single-cell RNA-seq data to tissue of origin   pp503 - 509 Kaia Achim, Jean-Baptiste Pettit, Luis R Saraiva, Daria Gavriouchkina, Tomas Larsson et al. doi:10.1038/nbt.3209 Single cells profiled by RNA-seq are rapidly assigned to their location in a complextissue using data in gene expression atlases. See also: News and Views by Faridani & Sandberg Research Top Articles Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers   pp510 - 517 Isaac B Hilton, Anthony M D'Ippolito, Christopher M Vockley, Pratiksha I Thakore, Gregory E Crawford et al. doi:10.1038/nbt.3199 RNA-guided epigenome editing with Cas9 fused to an acetyltransferase domain activates gene expression through modification of promoters and enhancers. A prevascularized subcutaneous device-less site for islet and cellular transplantation   pp518 - 523 Andrew R Pepper, Boris Gala-Lopez, Rena Pawlick, Shaheed Merani, Tatsuya Kin et al. doi:10.1038/nbt.3211 Diabetes reversal in mice is achieved by transplanting islets into a prevascularized space under the skin. See also: News and Views by Bromberg Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution OPEN   pp524 - 530 Fuguang Li, Guangyi Fan, Cairui Lu, Guanghui Xiao, Changsong Zou et al. doi:10.1038/nbt.3208 Two draft sequences of Gossypium hirsutum, the most widely cultivated cotton species, provide insights into genome structure, genome rearrangement, gene evolution and cotton fiber biology. See also: News and Views by Paterson & Wendel Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement OPEN   pp531 - 537 Tianzhen Zhang, Yan Hu, Wenkai Jiang, Lei Fang, Xueying Guan et al. doi:10.1038/nbt.3207 Two draft sequences of Gossypium hirsutum, the most widely cultivated cotton species, provide insights into genome structure, genome rearrangement, gene evolution and cotton fiber biology. See also: News and Views by Paterson & Wendel Letters Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining   pp538 - 542 Takeshi Maruyama, Stephanie K Dougan, Matthias C Truttmann, Angelina M Bilate, Jessica R Ingram et al. doi:10.1038/nbt.3190 The efficiency of homologous recombination-based Cas9 genome editing is increased by inhibiting non-homologous end joining. Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells   pp543 - 548 Van Trung Chu, Timm Weber, Benedikt Wefers, Wolfgang Wurst, Sandrine Sander et al. doi:10.1038/nbt.3198 The efficiency of precise CRISPR/Cas9 genome editing is increased by inhibition of the nonhomologous end joining pathway. Clearance of persistent hepatitis C virus infection in humanized mice using a claudin-1-targeting monoclonal antibody   pp549 - 554 Laurent Mailly, Fei Xiao, Joachim Lupberger, Garrick K Wilson, Philippe Aubert et al. doi:10.1038/nbt.3179 Inhibiting virus cell entry by targeting tight junctions cures HCV infection in mice. Resources C2H2 zinc finger proteins greatly expand the human regulatory lexicon   pp555 - 562 Hamed S Najafabadi, Sanie Mnaimneh, Frank W Schmitges, Michael Garton, Kathy N Lam et al. doi:10.1038/nbt.3128 Comprehensive analysis of the binding specificities of C2H2 zinc finger proteins reveals their DNA recognition code. Errata Erratum: Modeling the course of amyotrophic lateral sclerosis   p563 Christina Fournier and Jonathan D Glass doi:10.1038/nbt0515-563a Erratum: Selling long life   p563 Christopher Thomas Scott and Laura DeFrancesco doi:10.1038/nbt0515-563b Corrigendum Corrigendum: Taking charge of siRNA delivery   p563 Anastasia Khvorova, Maire F Osborn and Matthew R Hassler doi:10.1038/nbt0515-563c Careers and Recruitment Top First-quarter biotech job picture   p565 Michael Francisco doi:10.1038/nbt.3230 People People   p566 doi:10.1038/nbt.3232 Top Advertisement MSC Scientific Editing gives your research article a high-quality scientific edit that can help you make an impact on your intended audience. Nature-standard editing - English language edit included - Specialist editors in your subject area. Find out more today.    Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. 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