Nature Biotechnology Contents: Volume 33 pp 111 - 212

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TABLE OF CONTENTS February 2015 Volume 33, Issue 2 Editorial News Bioentrepreneur Opinion and Comment Features News and Views Computational Biology Research Careers and Recruitment Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement New White Paper: Literature Review of CHO vs. HEK Glycosylation Without careful evaluation of an expression system, data generated during preclinical development may lead to the progression of irrelevant candidates or overlooking promising ones, both of which will negatively impact the identification of the right drug candidate.  Download your copy today.   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement Nature Plants - Now launched  The first issue of Nature Plants has now published. Covering all aspects of plant science including genetics, cell and molecular biology, ecology, evolution, agriculture, biotechnology and economics. Explore the first issue - free access for a limited time.   Editorial Top Kicking the can   p111 doi:10.1038/nbt.3148 European politicians' decision to kick the can of genetically modified (GM) crop approvals down to national governments may accelerate adoption in the short term, but foreshadows legal battles down the road. News Top Roche swallows Ariosa, grabs slice of prenatal test market   pp113 - 114 Mark Ratner doi:10.1038/nbt0215-113 Pivotal trials for β-secretase inhibitors in Alzheimer's   pp115 - 116 Cormac Sheridan doi:10.1038/nbt0215-115 PARP inhibitor approved, despite vote   p116 doi:10.1038/nbt0215-116a Biogen Idec bets on chronic pain   p116 doi:10.1038/nbt0215-116b Merck buys BET family firm   p116 doi:10.1038/nbt0215-116c Antidotes progress for new oral clotbusters   pp117 - 118 Elie Dolgin doi:10.1038/nbt0215-117 23andMe sets sights on UK/Canada, signs up Genentech   p119 Asher Mullard doi:10.1038/nbt0215-119a Ebola+ and IMI 2   p119 doi:10.1038/nbt0215-119b UK catapults precision medicine   p119 doi:10.1038/nbt0215-119c Moderna's $450-million venture round   p119 doi:10.1038/nbt0215-119d Merck splashes on Cubist, antibiotics prospects brighten   pp120 - 121 Chris Morrison doi:10.1038/nbt0215-120 Around the world in a month   p121 doi:10.1038/nbt0215-121 Agricultural probiotics enter spotlight   p122 Jeffrey L Fox doi:10.1038/nbt0215-122 Data Page 2014—the biotech bull rages on   p123 Walter Yang doi:10.1038/nbt.3151 Drug pipeline: 4Q14   p124 Laura DeFrancesco doi:10.1038/nbt.3143 News Feature Fresh from the biotech pipeline—2014   pp125 - 128 Chris Morrison doi:10.1038/nbt.3136 Approvals of new chemical and biologic entities surged again in 2014, shepherded along by a confident FDA, as industry reaped the rewards of drug development incentives. Chris Morrison reports. Bioentrepreneur Top Building a business Incubators for innovators   pp129 - 132 Lynn Kirkpatrick doi:10.1038/nbt.3137 Podcast First Rounders Podcast: Carl Feldbaum   p132 doi:10.1038/nbt.3138 Opinion and Comment Top Correspondence Nuances in the entrepreneurship training tool box   pp133 - 134 Jan Rosier and David O'Connell doi:10.1038/nbt.3132 Response to Rosier Correspondence   p134 doi:10.1038/nbt.3135 Increasing patient participation in drug development   pp134 - 135 Paul Wicks, Maria Lowe, Susan Gabriel, Slaven Sikirica, Rahul Sasane et al. doi:10.1038/nbt.3145 Conflict of interests and evidence base for GM crops food/feed safety research   pp135 - 137 Miguel A Sanchez doi:10.1038/nbt.3133 Multi-omic data analysis using Galaxy   pp137 - 139 Jorrit Boekel, John M Chilton, Ira R Cooke, Peter L Horvatovich, Pratik D Jagtap et al. doi:10.1038/nbt.3134 A split-Cas9 architecture for inducible genome editing and transcription modulation   pp139 - 142 Bernd Zetsche, Sara E Volz and Feng Zhang doi:10.1038/nbt.3149 Features Top Patents Keeping score, strengthening policy and fighting bad actors over access to research tools   pp143 - 147 Tania Bubela and Robert Cook-Deegan doi:10.1038/nbt.3131 A mix of policy options that enhances access to research tools is available to courts, legislators and government bureaucracies, including research agencies and patent offices. Recent patent applications in single cell analysis   p148 doi:10.1038/nbt.3152 News and Views Top Illuminating Parkinson's therapy with optogenetics   pp149 - 150 Yuejun Chen, Man Xiong and Su-Chun Zhang doi:10.1038/nbt.3140 Controlling transplanted dopamine neurons with light helps define their mechanism of action. See also: Research by Steinbeck et al. Mapping the precision of genome editing   pp150 - 152 Richard Gabriel, Christof von Kalle and Manfred Schmidt doi:10.1038/nbt.3142 Genome-wide, unbiased methods provide a comprehensive picture of off-target cleavage by engineered nucleases. See also: Research by Wang et al. | Research by Frock et al. | Research by Tsai et al. Tracing the potential of lung progenitors   pp152 - 154 John E Mahoney and Carla F Kim doi:10.1038/nbt.3139 Transplanted lung progenitor cells give rise to specialized epithelial cells, raising new possibilities for treating lung disease. Research Highlights   p154 doi:10.1038/nbt.3144 Biotechnology JOBS of the week post-doc in microbial biotechnology Università Cattolica del Sacro Cuore Doctoral Students in Molecular Medicine, Neuroscience and Bioinformatics Institute for Molecular Medicine Finland (FIMM) Postdoctoral Fellow Position at Mount Sinai, New York Icahn School of Medicine at Mount Sinai Five Junior Research Group Leader Positions Leibniz Research Cluster (LRC) More Science jobs from Biotechnology EVENT Plant Genomes & Biotechnology: From Genes to Networks December 2, 2015 Cold Spring Harbor, USA More science events from Computational Biology Top Analysis Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells   pp155 - 160 Florian Buettner, Kedar N Natarajan, F Paolo Casale, Valentina Proserpio, Antonio Scialdone et al. doi:10.1038/nbt.3102 Hidden cell sub-populations are detected by accounting for confounding variation inthe analysis of single-cell RNA-seq data. Research Top Analysis Optimizing pyramided transgenic Bt crops for sustainable pest management   pp161 - 168 Yves Carriere, Neil Crickmore and Bruce E Tabashnik doi:10.1038/nbt.3099 This Analysis of data from 38 field studies identifies key factors affecting the durability of Bt toxin pyramids, and should inform future resistance management strategies. Large-scale test of the natural refuge strategy for delaying insect resistance to transgenic Bt crops   pp169 - 174 Lin Jin, Haonan Zhang, Yanhui Lu, Yihua Yang, Kongming Wu et al. doi:10.1038/nbt.3100 Combining four years of field data with computer modeling reveals that development of resistance to Bacillus thuringiensis insecticidal proteins (Bt) in cotton bollworm can be delayed by refuges of non-Bt host plants other than cotton, but that these so-called /`natural refuges/' are not as effective as non-Bt cotton refuges. Brief Communications Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors   pp175 - 178 Xiaoling Wang, Yebo Wang, Xiwei Wu, Jinhui Wang, Yingjia Wang et al. doi:10.1038/nbt.3127 Off-target cleavage by CAS9 or TALEN genome editing tools is detected by integrase-defective lentiviral vectors. See also: News and Views by Gabriel et al. Articles Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases   pp179 - 186 Richard L Frock, Jiazhi Hu, Robin M Meyers, Yu-Jui Ho, Erina Kii et al. doi:10.1038/nbt.3101 An unbiased genome-wide method reveals on- and off-target DNA cleavage by TALEN and Cas9 nucleases by detecting chromosome translocation events. See also: News and Views by Gabriel et al. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases   pp187 - 197 Shengdar Q Tsai, Zongli Zheng, Nhu T Nguyen, Matthew Liebers, Ved V Topkar et al. doi:10.1038/nbt.3117 An unbiased approach for the genome-wide detection of off-target cleavage by CRISPR-Cas9 RNA-guided nucleases reveals wide variability in the off-target activity of different guide RNAs. See also: News and Views by Gabriel et al. Genomic discovery of potent chromatin insulators for human gene therapy   pp198 - 203 Mingdong Liu, Matthew T Maurano, Hao Wang, Heyuan Qi, Chao-Zhong Song et al. doi:10.1038/nbt.3062 A genome-wide analysis of CTCF binding sites yields compact sequence elements that function as chromatin insulators. Letter Optogenetics enables functional analysis of human embryonic stem cell-derived grafts in a Parkinson's disease model   pp204 - 209 Julius A Steinbeck, Se Joon Choi, Ana Mrejeru, Yosif Ganat, Karl Deisseroth et al. doi:10.1038/nbt.3124 Optogenetics helps unravel how neural cell grafts ameliorate symptoms in a mouse model of Parkinson's disease. See also: News and Views by Chen et al. Errata Erratum: Reinventing tech transfer   p210 Brady Huggett doi:10.1038/nbt0215-210a Erratum: Bring out your dead   p210 doi:10.1038/nbt0215-210b Corrigenda Corrigendum: Linking T-cell receptor sequence to functional phenotype at the single-cell level   p210 Arnold Han, Jacob Glanville, Leo Hansmann and Mark M Davis doi:10.1038/nbt0215-210c Corrigendum: Selling long life   p210 Christopher Thomas Scott and Laura DeFrancesco doi:10.1038/nbt0215-210d Corrigendum: Status and market potential of transgenic biofortified crops   p210 Hans De Steur, Dieter Blancquaert, Simon Strobbe, Willy Lambert, Xavier Gellynck et al. doi:10.1038/nbt0215-210e Careers and Recruitment Top Fourth-quarter biotech job picture   p211 Michael Francisco doi:10.1038/nbt.3141 People People   p212 doi:10.1038/nbt.3153 Top Advertisement Nature Collections Nobel Prize in Chemistry 2014 The 2014 Nobel Prize in Chemistry was awarded for the development of super-resolution fluorescence microscopy. 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