Nature Biotechnology Contents: Volume 32 pp 1169 - 1282

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TABLE OF CONTENTS December 2014 Volume 32, Issue 12 Editorial News News Feature Bioentrepreneur Opinion and Comment Feature News and Views Computational Biology Research Careers and Recruitment Advertisement   NEW WHITE PAPER: Application of CRISPR/Cas-based Technology to the Rapid Generation of Genetically Engineered Mice  Download this White Paper, to Learn More About: Gene Editing vs. Gene Manipulation How CRISPR/Cas-based Gene Editing Works The Advantages and Limitations of Gene Editing Technologies And, In Vivo Gene Editing Technology versus Conventional Genetic Engineering Methods Download Your Copy Today  Japanese table of contents Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement Nature Collections RNA SEQUENCING QUALITY CONTROL (SEQC) Articles from Nature Biotechnology, Nature Communications and Scientific Data presents results from The RNA Sequencing Quality Control (SEQC) project. This is the second phase of a collaboration between government, academic and industry researchers as part of the MicroArray Quality Control (MAQC) consortium. Produced with support from the FDA's National Center for Toxicological Research (NCTR/FDA)  and the State Key Laboratory of Genetic Engineering (SKLGE) at Fudan University   Editorial Top Label without a cause   p1169 doi:10.1038/nbt.3094 Mandatory labeling of GM food in the United States will not only make all food more costly but also bamboozle consumers. News Top Developers seek to finetune toxicity of T-cell therapies   pp1171 - 1172 Chris Morrison doi:10.1038/nbt1214-1171 Stem cell therapy clears first hurdle in AMD   pp1173 - 1174 Cormac Sheridan doi:10.1038/nbt1214-1173 Celgene wagers on Sutro's cell-free platform to ramp up ADCs   p1175 Mark Ratner doi:10.1038/nbt1214-1175 Dendreon bankrupt   p1176 doi:10.1038/nbt1214-1176a New drug costs soar to $2.6 billion   p1176 doi:10.1038/nbt1214-1176b Nagoya Protocol takes effect—without Japan   p1176 doi:10.1038/nbt1214-1176c Listeria vaccines join the checkpoint frenzy   pp1176 - 1177 Gunjan Sinha doi:10.1038/nbt1214-1176d Checkpoint IDO draws $1 billion   p1177 doi:10.1038/nbt1214-1177a Pfizer joins PD-L1 pursuit   p1177 doi:10.1038/nbt1214-1177b Novartis unloads flu vaccine unit   p1177 doi:10.1038/nbt1214-1177c Once-yearly device takes on daily and weekly diabetes drugs   p1178 Asher Mullard doi:10.1038/nbt1214-1178 Early-access scheme picks controversial glioblastoma vaccine   p1179 Suzanne Elvidge doi:10.1038/nbt1214-1179a Around the world in a month   p1179 doi:10.1038/nbt1214-1179b News Feature Top Labeling for better or worse   pp1180 - 1183 Jim Kling doi:10.1038/nbt.3087 As US legislatures and voters continue to wrestle with GM labeling, what is at stake? Jim Kling investigates. Bioentrepreneur Top Feature Reinventing tech transfer   pp1184 - 1191 Brady Huggett doi:10.1038/nbt.3085 First Rounders Podcast: Daphne Zohar   p1191 doi:10.1038/nbt.3088 Opinion and Comment Top Correspondence The business case for cell and gene therapies   pp1192 - 1193 Mohamed Abou-El-Enein, Gerhard Bauer and Petra Reinke doi:10.1038/nbt.3084 Feature Top Patents Who owns CRISPR-Cas9 in Europe?   pp1194 - 1196 Andras Kupecz doi:10.1038/nbt.3086 Recent developments in the prosecution of the CRISPR-Cas9 patent in Europe highlight the differences between the intellectual property regimes of Europe and the United States. News and Views Top Taking charge of siRNA delivery   pp1197 - 1198 Anastasia Khvorova, Maire F Osborn and Matthew R Hassler doi:10.1038/nbt.3091 Delivery of siRNA into cells is achieved by neutralizing the negative charge of the phosphate backbone in a reversible manner. See also: Research by Meade et al. Lightening the load in synthetic biology   pp1198 - 1200 Eric Klavins doi:10.1038/nbt.3089 A new biological device known as a 'load driver' improves the performance of synthetic circuits by insulating genetic parts from each other. See also: Research by Mishra et al. Research Highlights   p1200 doi:10.1038/nbt.3090 What's in a stomach?   p1201 Irene Jarchum doi:10.1038/nbt.3092 Biotechnology JOBS of the week Postdoc Position in Stem Cells & Cortical Development University of California - Irvine Professor (All Levels) in Metabolic Engineering King Abdullah University of Science and Technology (KAUST) Funded PhD Studentships London Interdisciplinary Biosciences PhD Programme Tier 1 Canada Research Chair in Data Science University of British Columbia (UBC) More Science jobs from Biotechnology EVENT Biotechnology for the Non-Biotechnologist April 22, 2015 Nice, France More science events from Computational Biology Top Analysis A community effort to assess and improve drug sensitivity prediction algorithms   pp1202 - 1212 James C Costello, Laura M Heiser, Elisabeth Georgii, Mehmet Gonen, Michael P Menden et al. doi:10.1038/nbt.2877 A community of researchers report the lessons learned from applying 44 algorithms to predict drug sensitivity in cancer cell lines using genomic, epigenetic and proteomic datasets A community computational challenge to predict the activity of pairs of compounds   pp1213 - 1222 Mukesh Bansal, Jichen Yang, Charles Karan, Michael P Menden, James C Costello et al. doi:10.1038/nbt.3052 A community computational challenge generates algorithms to predict activity of drug combinations. Research Top Articles Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells   pp1223 - 1230 Weida Li, Claudia Cavelti-Weder, Yinying Zhang, Kendell Clement, Scott Donovan et al. doi:10.1038/nbt.3082 Efficient reprogramming of pancreatic acinar cells in vivo generates cells that mature toward a beta cell phenotype over many months. Single-base resolution analysis of active DNA demethylation using methylase-assisted bisulfite sequencing   pp1231 - 1240 Hao Wu, Xiaoji Wu, Li Shen and Yi Zhang doi:10.1038/nbt.3073 Methylase-assisted bisulfite sequencing allows to determine the genomic locations of the cytosine demethylation intermediates 5-formylcytosine and 5-carboxylcytosine at base pair resolution. Functional optimization of gene clusters by combinatorial design and assembly   pp1241 - 1249 Michael J Smanski, Swapnil Bhatia, Dehua Zhao, YongJin Park, Lauren B A Woodruff et al. doi:10.1038/nbt.3063 Combinatorial replacement and rearrangement of genetic parts in a refactored nitrogen fixation gene cluster lead to improved performance. Letters The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease OPEN   pp1250 - 1255 Xinxia Peng, Jessica Alfoldi, Kevin Gori, Amie J Eisfeld, Scott R Tyler et al. doi:10.1038/nbt.3079 The genome sequence of the ferret, a model of human respiratory disease, enables research on influenza and cystic fibrosis. Efficient delivery of RNAi prodrugs containing reversible charge-neutralizing phosphotriester backbone modifications   pp1256 - 1261 Bryan R Meade, Khirud Gogoi, Alexander S Hamil, Caroline Palm-Apergi, Arjen van den Berg et al. doi:10.1038/nbt.3078 Neutralizing the RNA phosphodiester backbone enables delivery of siRNA across cell membranes. See also: News and Views by Khvorova et al. Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation   pp1262 - 1267 John G Doench, Ella Hartenian, Daniel B Graham, Zuzana Tothova, Mudra Hegde et al. doi:10.1038/nbt.3026 Analysis of the genome editing activity of more than 1800 sgRNAs in mouse and human cells yields rules to facilitate design of highly active RNA guides for Cas-9 genome editing. A load driver device for engineering modularity in biological networks   pp1268 - 1275 Deepak Mishra, Phillip M Rivera, Allen Lin, Domitilla Del Vecchio and Ron Weiss doi:10.1038/nbt.3044 Including a load driver in a modular genetic circuit abrogates the effect of downstream elements that can interfere with circuit performance. See also: News and Views by Klavins Tunable protein degradation in bacteria   pp1276 - 1281 D Ewen Cameron and James J Collins doi:10.1038/nbt.3053 An orthogonal protein degradation system in bacteria provides control of both protein concentration and the protein degradation rate. Careers and Recruitment Top People People   p1282 doi:10.1038/nbt.3097 Advertisement Nature Outlook: Medical Research Masterclass From the basic functions of the cell to cures for HIV and cancer, Nature Outlook: Medical Research Masterclass uses the 2014 Lindau Nobel Laureate Meeting as a basis to explore the latest research in medicine and physiology. Access the Outlook free online for six months.  Supported by Mars, Incorporated    Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

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