Laboratory Investigation - Table of Contents alert Volume 93 Issue 2

TABLE OF CONTENTS Volume 93, Issue 2 (February 2013) In this issue Inside LI Editorial Research Articles Corrigendum Also new AOP Sign up for e-alerts Recommend to your library Web feed Subscribe Advertisement This month's recommended article by the Editors of LI: The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function Open   Inside LI Top Inside Lab Invest 2013 93: 146-147; 10.1038/labinvest.2012.181 Full Text Editorial Top Real estate of monolayer permeability: location location location Corey Hardin, Jeffrey J Fredberg and Ramaswamy Krishnan 2013 93: 148-150; 10.1038/labinvest.2012.168 Full Text Research Articles Top ANGIOGENESIS, CARDIOVASCULAR AND PULMONARY SYSTEMS Transgenic mouse model with deficient mitochondrial polymerase exhibits reduced state IV respiration and enhanced cardiac fibrosis This paper explores cardiac mitochondrial function and fibrosis in a cardiacspecific transgenic mouse model with a mutant mitochondrial polymerase (pol γ). Pol γ transgenic mouse hearts exhibit cardiac fibrosis, reduced mitochondrial DNA abundance, and reduced mitochondrial function. Therefore, defective pol γ function promotes cardiomyopathy and reduced mitochondrial energy. Christopher A Koczor, Rebecca A Torres, Earl Fields, Qianhong Qin, Jade Park, Tomika Ludaway, Rodney Russ and William Lewis 2013 93: 151-158; advance online publication, October 22, 2012; 10.1038/labinvest.2012.146 Abstract | Full Text KCa3.1 channels mediate the increase of cell migration and proliferation by advanced glycation endproducts in cultured rat vascular smooth muscle cells Advanced glycation endproducts (AGEs) increase the expression of intermediateconductance Ca2+-activated K+ channels in an AGE receptor-dependent manner by activating ERK1/2, P38-MAPK and PI3K signal pathways. AGEs therefore promote vascular smooth muscle cell migration and proliferation, which may play a role in the development of diabetic vascular complications. Li-Mei Zhao, Xing-Li Su, Yan Wang, Gui-Rong Li and Xiu-Ling Deng 2013 93: 159-167; advance online publication, November 19, 2012; 10.1038/labinvest.2012.163 Abstract | Full Text Delayed preconditioning prevents ischemia/reperfusion-induced endothelial injury in rats: role of ROS and eNOS This paper describes the mechanism that mediates delayed preconditioninginduced endothelial protection against ischemia/reperfusion injury in peripheral arteries. Protection is associated with inhibition of oxidative stress and activation of the PI3K/Akt/eNOS pathway. Thus, remote ischemic preconditioning, elicited by ischemia of easily accessible tissues, may protect remote vital organs undergoing ischemia/reperfusion. Xi He, Ming Zhao, Xue-Yuan Bi, Xiao-Jiang Yu and Wei-Jin Zang 2013 93: 168-180; advance online publication, November 12, 2012; 10.1038/labinvest.2012.160 Abstract | Full Text GENITOURINARY AND REPRODUCTIVE SYSTEMS The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function Open This study reveals the restorative effects of adipose-derived mesenchymal stem cells (A-MSCs) on damaged ovaries. A-MSCs are capable of inducing angiogenesis and increasing the number of follicles and corpus lutea, as well as elevating expression levels of VEGF, IGF-1, HGF and StAR in ovaries after their transplantation. These results suggest that A-MSCs could be beneficial in regeneration of malfunctioning ovaries. Yuji Takehara, Akiko Yabuuchi, Kenji Ezoe, Tomoko Kuroda, Rie Yamadera, Chiaki Sano, Nana Murata, Takuya Aida, Ken Nakama, Fumihito Aono, Naoki Aoyama, Keiich Kato and Osamu Kato 2013 93: 181-193; advance online publication, November 19, 2012; 10.1038/labinvest.2012.167 Abstract | Full Text Effects of dexamethasone on the TGF-β1-induced epithelial-to-mesenchymal transition in human peritoneal mesothelial cells Epithelial-to-mesenchymal transition (EMT) of peritoneal mesothelial cell (HPMC) is a key mechanism of peritoneal fibrosis. Because glucocorticoids inhibit the deposition of extracellular matrix, they are a potential treatment for peritoneal fibrosis. This paper shows that glucocorticoids exert a protective effect on EMT in TGF-β1-stimulated HPMC by the regulation of MAPK signaling and expression of TGF-β receptors. Yang-Hee Jang, Hyun-Soo Shin, Hack Sun Choi, Eun-Sun Ryu, Mi Jin Kim, Seog Ki Min, Joo-Ho Lee, Hyeon Kook Lee, Kwang-Ho Kim and Duk-Hee Kang 2013 93: 194-206; advance online publication, December 3, 2012; 10.1038/labinvest.2012.166 Abstract | Full Text ENDOCRINE, VISUAL AND AUDITORY SYSTEMS Impaired cornea wound healing in a tenascin C-deficient mouse model Genetic ablation of the extracellular matrix molecule tenascin C, impairs primary tissue repair in the corneal stroma following incision injury. In wounded tenascin C-null mouse corneas, there is suppression of fibrogenic gene expression, a reduction in the number of myofibroblasts, and decreased macrophage invasion. Takayoshi Sumioka, Ai Kitano, Kathleen C Flanders, Yuka Okada, Osamu Yamanaka, Norihito Fujita, Hiroki Iwanishi, Winston W-Y Kao and Shizuya Saika 2013 93: 207-217; advance online publication, December 3, 2012; 10.1038/labinvest.2012.157 Abstract | Full Text HEPATIC AND PANCREATIC SYSTEMS Carbon tetrachloride-induced hepatic injury through formation of oxidized diacylglycerol and activation of the PKC/NF-κB pathway Protein kinase C (PKC) signal transduction depends on diacylglycerol (DAG). DAG peroxides (DAG-O(O)H) activate PKC more strongly than unoxidized DAG, suggesting that DAG-O(O)H, if generated under oxidative stress, could act as an aberrant signal transducer. This study shows that DAG-O(O)H is generated in the process of CCl4-induced liver injury, activating the PKC/NF-κB pathway and TNFα-mediated liver injury. Kentaro Toriumi, Yosuke Horikoshi, R Yoshiyuki Osamura, Yorihiro Yamamoto, Naoya Nakamura and Susumu Takekoshi 2013 93: 218-229; advance online publication, December 3, 2012; 10.1038/labinvest.2012.145 Abstract | Full Text Spontaneous onset of nonalcoholic steatohepatitis and hepatocellular carcinoma in a mouse model of metabolic syndrome TSOD male mice spontaneously develop non-alcoholic steatohepatitis (NASH) and hepatocellular carcinoma without any additional treatment. The pathological findings in the livers of these mice mimic those seen in patients with NASH. These mice therefore show promise as a model for assessing NASH and NASH-induced carcinogenesis due to metabolic syndrome. Takeshi Nishida, Koichi Tsuneyama, Makoto Fujimoto, Kazuhiro Nomoto, Shinichi Hayashi, Shigeharu Miwa, Takahiko Nakajima, Yuko Nakanishi, Yoshiyuki Sasaki, Wataru Suzuki, Seiichi Iizuka, Mitsunobu Nagata, Tsutomu Shimada, Masaki Aburada, Yutaka Shimada and Johji Imura 2013 93: 230-241; advance online publication, November 19, 2012; 10.1038/labinvest.2012.155 Abstract | Full Text MODELS AND TECHNIQUES Intrinsic indicators for specimen degradation Cell lines and human tissue specimens were exposed to an experimental cold ischemic time-dependent tissue degradation model. Using two-dimensional fluorescence gel electrophoresis in conjunction with mass spectrometry, tissue degradation indicators were identified for quantitative measurement of specimen degradation. These findings may allow for development of novel tests for quality control of human biospecimens. Jie Li, Catherine Kil, Kelly Considine, Bartosz Smarkucki, Michael C Stankewich, Brian Balgley and Alexander O Vortmeyer 2013 93: 242-253; advance online publication, November 19, 2012; 10.1038/labinvest.2012.164 Abstract | Full Text Measurement of local permeability at subcellular level in cell models of agonist- and ventilator-induced lung injury Alterations of cell monolayer permeability are found in several pathological conditions, including atherosclerosis, stroke, lung injury, cancer and digestive disorders. This study describes a novel methodology that allows visualization and quantitation of local permeability at the subcellular level in static cell monolayers and in cells exposed to active mechanochemical environments. Oleksii Dubrovskyi, Anna A Birukova and Konstantin G Birukov 2013 93: 254-263; advance online publication, November 19, 2012; 10.1038/labinvest.2012.159 Abstract | Full Text Corrigendum Top Sonic Hedgehog contributes to gastric mucosal restitution after injury Chang Xiao, Rui Feng, Amy C Engevik, Jason R Martin, Julie A Tritschler, Michael Schumacher, Robert Koncar, Joseph Roland, Ki Taek Nam, James R Goldenring and Yana Zavros 2013 93: 264; 10.1038/labinvest.2012.170 Full Text Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to Laboratory Investigation. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/nams/svc/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department. For print subscription enquiries, please contact our subscription department. For other enquiries, please contact our customer feedback department. Nature Publishing Group | 75 Varick Street, 9th Floor | New York | NY 10013-1917 | USA Nature Publishing Group's worldwide offices: London - Paris - Munich - New Delhi - Tokyo - Melbourne San Diego - San Francisco - Washington - New York - Boston Macmillan Publishers Limited is a company incorporated in England and Wales under company number 785998 and whose registered office is located at Brunel Road, Houndmills, Basingstoke, Hampshire RG21 6XS. © 2013 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.