Laboratory Investigation - Table of Contents alert Volume 95 Issue 7

If you are unable to see the message below, click here to view.

TABLE OF CONTENTS Volume 95, Issue 7 (July 2015) In this issue Inside the USCAP Journals Research Articles Technical Report Also new AOP Sign up for e-alerts Recommend to your library Web feed Subscribe Inside the USCAP Journals Top Inside the USCAP Journals 2015 95: 700-701; 10.1038/labinvest.2015.75 Full Text Research Articles Top BREAST, SKIN, SOFT TISSUE AND BONE Aspirin blocks growth of breast tumor cells and tumor-initiating cells and induces reprogramming factors of mesenchymal to epithelial transition This paper elucidates the role of aspirin in prevention of breast carcinogenesis. Aspirin reduces breast cancer cell growth and the self-renewal capacity/stemness of breast tumor-initiating cells by regulation of reprogramming factors of mesenchymal to epithelial transition via TGF-β signaling. Gargi Maity, Archana De, Amlan Das, Snigdha Banerjee, Sandipto Sarkar and Sushanta K Banerjee 2015 95: 702-717; advance online publication, April 13, 2015; 10.1038/labinvest.2015.49 Abstract | Full Text ORAL AND GASTROINTESTINAL SYSTEMS CDX2 homeoprotein is involved in the regulation of ST6GalNAc-I gene in intestinal metaplasia Expression of the sialyl-Tn antigen is one of the most common features of intestinal metaplasia and gastric carcinomas. This study reveals that the CDX2 homeoprotein transcriptionally regulates the α2,6-sialyltransferase ST6GalNAc-I gene, which is responsible for cancer-associated Sialyl-Tn expression. Rita Pinto, Rita Barros, Isabel Pereira-Castro, Patricia Mesquita, Luis T da Costa, Eric P Bennett, Raquel Almeida and Leonor David 2015 95: 718-727; advance online publication, April 13, 2015; 10.1038/labinvest.2015.52 Abstract | Full Text Microangiopathy triggers, and inducible nitric oxide synthase exacerbates dextran sulfate sodium-induced colitis This study shows that dextran sulfate sodium induced colitis in mice is triggered by microcirculatory disturbances in the mucosa and is aggravated by excessive neuronal inducible nitric oxide synthase (iNOS). Inhibition of neuronal iNOS thus appears to be a target for treatment of colitis. Hiroki Saijo, Norifumi Tatsumi, Seiji Arihiro, Tomohiro Kato, Masataka Okabe, Hisao Tajiri and Hisashi Hashimoto 2015 95: 728-748; advance online publication, May 4, 2015; 10.1038/labinvest.2015.60 Abstract | Full Text ANGIOGENESIS, CARDIOVASCULAR AND PULMONARY SYSTEMS Receptor role of the annexin A2 in the mesothelial endocytosis of crocidolite fibers Mesothelial phagocytosis of asbestos is involved in the pathogenesis of mesothelioma. The authors screened membrane proteins of MeT5A mesothelial cells that were adsorbed on crocidolite or chrysotile to identify phagocytic receptors, and identified the phospholipid binding protein annexin A2 as a candidate. Knockdown and receptor blocking of annexin A2 significantly suppresses mesothelial phagocytosis of crocidolite, verifying its potential role as a receptor for asbestos. Kyoko Yamashita, Hirotaka Nagai and Shinya Toyokuni 2015 95: 749-764; advance online publication, April 27, 2015; 10.1038/labinvest.2015.28 Abstract | Full Text A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway Microvascular endothelial cells cultured in three-dimensional hydrogel scaffolds form a network of microvessel structures that, when implanted in mice, inosculate with host vessels and remodel into large ectatic vascular structures resembling hemangiomas. This implant model has potential for testing and development of therapeutics targeting infantile hemangiomas, reducing the scarring and deformities sometimes associated with these lesions. Masayuki Tsuneki, Steven Hardee, Michael Michaud, Raffaella Morotti, Erin Lavik and Joseph A Madri 2015 95: 765-780; advance online publication, May 11, 2015; 10.1038/labinvest.2015.61 Abstract | Full Text HEPATIC AND PANCREATIC SYSTEMS MicroRNA-17-5p activates hepatic stellate cells through targeting of Smad7 This paper shows that microRNA (miR)-17-5p expression is increased during liver fibrosis and is associated with transforming growth factor (TGF)-β1-induced expression of type I collagen and α-smooth muscle actin in hepatic stellate cells (HSC). Smad7, a negative regulator of the TGF-β/Smad pathway, was confirmed as a direct target of miR-17-5p, supporting its potential utility as a therapeutic target for liver fibrosis. Fujun Yu, Yong Guo, Bicheng Chen, Peihong Dong and Jianjian Zheng 2015 95: 781-789; advance online publication, April 27, 2015; 10.1038/labinvest.2015.58 Abstract | Full Text Curcumin regulates cell fate and metabolism by inhibiting hedgehog signaling in hepatic stellate cells Curcumin is a natural product with anti-fibrotic properties. The authors show that curcumin causes cell cycle arrest, induces apoptosis and inhibits fibrogenesis in hepatic stellate cells by suppressing hedgehog signaling. Disruption of hedgehog signaling is also required for curcumin to inhibit glycolysis and regulate metabolism in these cells. These findings provide the molecular basis for developing curcumin as a therapy for liver fibrosis. Naqi Lian, Yuanyuan Jiang, Feng Zhang, Huanhuan Jin, Chunfeng Lu, Xiafei Wu, Yin Lu and Shizhong Zheng 2015 95: 790-803; advance online publication, May 4, 2015; 10.1038/labinvest.2015.59 Abstract | Full Text Estrogen suppresses hepatocellular carcinoma cells through ERβ-mediated upregulation of the NLRP3 inflammasome The incidence of hepatocellular carcinoma (HCC) is higher in males than in females, which suggests a role for sex hormones in pathogenesis. The present study shows that estrogen receptor (ER)β expression correlates negatively with disease progression, and correlates positively with expression of NLRP3 inflammasome components. Treatment with 17β-estradiol (E2) inhibits the malignant behavior of HCC cells through E2/ERβ/MAPK pathway-mediated upregulation of the NLRP3 inflammasome. Qing Wei, Pengbo Guo, Kun Mu, Ying Zhang, Wei Zhao, Wanwan Huai, Yumin Qiu, Tao Li, Xiaomin Ma, Yafei Liu, Xiaoyan Chen and Lihui Han 2015 95: 804-816; advance online publication, May 25, 2015; 10.1038/labinvest.2015.63 Abstract | Full Text GENITOURINARY AND REPRODUCTIVE SYSTEMS MicroRNA-129-5p modulates epithelial-to-mesenchymal transition by targeting SIP1 and SOX4 during peritoneal dialysis Long-term peritoneal dialysis (PD) as renal replacement therapy can induce mesothelial/epithelial-mesenchymal transition (MMT/EMT) and fibrosis, eventually leading to ultrafiltration failure. This study indicates that overexpression of microRNA-129-5p (miR-129-5p) can protect against MMT/EMT through SIP1, a member of the zinc-finger E-box-binding homeobox factor family; and SOX4, the sex-determining region Y-related high-mobility group box 4 gene. These findings may yield a therapeutic intervention for peritoneal fibrosis in PD. Li Xiao, Xun Zhou, Fuyou Liu, Chun Hu, Xuejing Zhu, Ying Luo, Ming Wang, Xiaoxuan Xu, Shikun Yang, Yashpal S Kanwar and Lin Sun 2015 95: 817-832; advance online publication, May 11, 2015; 10.1038/labinvest.2015.57 Abstract | Full Text Technical Report Top MODELS AND TECHNIQUES Formalin-fixed, paraffin-embedded (FFPE) tissue epigenomics using Infinium HumanMethylation450 BeadChip assays This paper describes a DNA restoration protocol that provides robust, accurate and reproducible results with formalin-fixed, paraffin-embedded (FFPE) tissue-derived DNA, which are comparable to those obtained with fresh-frozen (FF) tissue. Differentially methylated genes can be validated using more sensitive techniques, such as nested methylation-specific PCR, together providing an epigenomics platform for molecular pathological epidemiology research on archived samples with limited tissue. Tim C de Ruijter, Joep PJ de Hoon, Jeroen Slaats, Bart de Vries, Marjolein JFW Janssen, Tom van Wezel, Maureen JB Aarts, Manon van Engeland, Vivianne CG Tjan-Heijnen, Leander Van Neste and Jürgen Veeck 2015 95: 833-842; advance online publication, April 13, 2015; 10.1038/labinvest.2015.53 Abstract | Full Text Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to Laboratory Investigation. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/nams/svc/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department. For print subscription enquiries, please contact our subscription department. For other enquiries, please contact our customer feedback department. Nature Publishing Group | 75 Varick Street, 9th Floor | New York | NY 10013-1917 | USA Nature Publishing Group's worldwide offices: London - Paris - Munich - New Delhi - Tokyo - Melbourne San Diego - San Francisco - Washington - New York - Boston Macmillan Publishers Limited is a company incorporated in England and Wales under company number 785998 and whose registered office is located at Brunel Road, Houndmills, Basingstoke, Hampshire RG21 6XS. © 2015 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.